Lab-on-a-Disc Platform for Automated Chemical Cell Lysis

Abstract

Chemical cell lysis is an interesting topic in the research to Lab-on-a-Disc (LOD) platforms on account of its perfect compatibility with the centrifugal spin column format. However, standard procedures followed in chemical cell lysis require sophisticated non-contact temperature control as well as the use of pressure resistant valves. These requirements pose a significant challenge thereby making the automation of chemical cell lysis on an LOD extremely difficult to achieve. In this study, an LOD capable of performing fully automated chemical cell lysis is proposed, where a combination of chemical and thermal methods has been used. It comprises a sample inlet, phase change material sheet (PCMS)-based temperature sensor, heating chamber, and pressure resistant valves. The PCMS melts and solidifies at a certain temperature and thus is capable of indicating whether the heating chamber has reached a specific temperature. Compared to conventional cell lysis systems, the proposed system offers advantages of reduced manual labor and a compact structure that can be readily integrated onto an LOD. Experiments using Salmonella typhimurium strains were conducted to confirm the performance of the proposed cell lysis system. The experimental results demonstrate that the proposed system has great potential in realizing chemical cell lysis on an LOD whilst achieving higher throughput in terms of purity and yield of DNA thereby providing a good alternative to conventional cell lysis systems.

Bacterial Cell Cultures in a Lab-on-a-Disc: A Simple and Versatile Tool for Quantification of Antibiotic Treatment Efficacy

“…By avoiding the use of pumps and tubing, it is possible to significantly decrease the introduction of bubbles in the system, and thereby bubble traps, which are commonly present in other fluidic systems, are not needed. Additionally, there are various methods to integrate multiple operational units such as filtration, metering, mixing, and valving to enable implementation of complex chemical and biological assays. , LoD systems have been used for a wide range of applications including diagnostics, , food analysis, sample pretreatment, and cell handling . There are only a few reports on application of centrifugal microfluidics for studying cells and living organisms.…”

Developing a surface acoustic wave‐induced microfluidic cell lysis device for point‐of‐care DNA amplification

“…Thermal lysis presents an attractive option due to the ease of integrating polymerase chain reaction (PCR) modules into microfluidic devices [ 3 , 7 ]. While chemical lysis in chip devices may present challenges, such as complex injection channel structures, reagent delivery time, and post‐lysis washing steps, these chemical lysis‐based chips offer substantial systems capable of achieving high‐throughput lysis, thereby reducing processing time and manual labor [ 10 , 11 , 12 ]. Additionally, chip‐based devices have the potential to employ high‐energy sound waves, strong electric fields, lasers, and optically induced dielectrophoretic techniques [ 3 ].…”