Lab-on-chip for liquid biopsy (LoC-LB) based on dielectrophoresis

Mathew, Bobby; Alazzam, Anas; Khashan, Saud; Abutayeh, Mohammad

doi:10.1016/j.talanta.2016.11.008

Cited by 35 publications

(22 citation statements)

References 18 publications

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“…The rGO film is patterned to create two electrodes which are then connected to an AC signal to generate inhomogeneous electric device using a syringe pump. The channel fabrication, medium and samples preparations, are detailed in [40][41][42]. Figure 5 shows the pDEP response of RBCs under an AC signal of 5 Vpp at 1 MHz.…”

Section: Living Cells Manipulationmentioning

confidence: 99%

Micro-Pattern of Graphene Oxide Films Using Metal Bonding

et al. 2020

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Recently, graphene has been explored in several research areas according to its outstanding combination of mechanical and electrical features. The ability to fabricate micro-patterns of graphene facilitates its integration in emerging technologies such as flexible electronics. This work reports a novel micro-pattern approach of graphene oxide (GO) film on a polymer substrate using metal bonding. It is shown that adding ethanol to the GO aqueous dispersion enhances substantially the uniformity of GO thin film deposition, which is a great asset for mass production. On the other hand, the presence of ethanol in the GO solution hinders the fabrication of patterned GO films using the standard lift-off process. To overcome this, the fabrication process provided in this work takes advantage of the chemical adhesion between the GO or reduced GO (rGO) and metal films. It is proved that the adhesion between the metal layer and GO or rGO is stronger than the adhesion between the latter and the polymer substrate (i.e., cyclic olefin copolymer used in this work). This causes the removal of the GO layer underneath the metal film during the lift-off process, leaving behind the desired GO or rGO micro-patterns. The feasibility and suitability of the proposed pattern technique is confirmed by fabricating the patterned electrodes inside a microfluidic device to manipulate living cells using dielectrophoresis. This work adds great value to micro-pattern GO and rGO thin films and has immense potential to achieve high yield production in emerging applications.

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Section: Living Cells Manipulationmentioning

confidence: 99%

Micro-Pattern of Graphene Oxide Films Using Metal Bonding

et al. 2020

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“…WBCs mixed with CTCs Up to 92% recovery of CTCs; CTCs recovery inversely correlated with the chamber load and the isolation efficiency positively correlated with chamber size [167] Two sets of interdigitated electrodes on each side of the microfluidic channel and a pillar in the middle focus the flow in the vicinity of the electrodes MDA-MB-231 spiked in blood DEP-induced lateral displacement of cells [129] The top electrode of ITO, the bottom electrode covered the V-shape define also the microfluidic channel PC14PE6/AS2-GFP (AS2-GFP) lung cancer cells High throughput DEP method; the continuous cell sorting allowed the processing of 5 mL of sample in 2 h, at 85% recovery rate and at a purity greater than 90% (for an enrichment factor of 10 5 ) [130] Concentric circular electrodes…”

Section: Mda-231 Cancer Cellsmentioning

confidence: 99%

Highlighting the uniqueness in dielectrophoretic enrichment of circulating tumor cells

et al. 2019

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Circulating tumor cells (CTCs) play an essential role in the metastasis of tumors, and thus can serve as a valuable prognostic factor for malignant diseases. As a result, the ability to isolate and characterize CTCs is essential. This review underlines the potential of dielectrophoresis for CTCs enrichment. It begins by summarizing the key performance parameters and challenges of CTCs isolation using microfluidics. The two main categories of CTCs enrichment—affinity‐based and label‐free methods—are analysed, emphasising the advantages and disadvantages of each as well as their clinical potential. While the main argument in favour of affinity‐based methods is the strong specificity of CTCs isolation, the major advantage of the label‐free technologies is in preserving the integrity of the cellular membrane, an essential requirement for downstream characterization. Moving forward, we try to answer the main question: “What makes dielectrophoresis a method of choice in CTCs isolation?” The uniqueness of dielectrophoretic CTCs enrichment resides in coupling the specificity of the isolation process with the conservation of the membrane surface. The specificity of the dielectrophoretic method stems from the differences in the dielectric properties between CTCs and other cells in the blood: the capacitances of the malignantly transformed cellular membranes of CTCs differ from those of other cells. Examples of dielectrophoretic devices are described and their performance evaluated. Critical requirements for using dielectrophoresis to isolate CTCs are highlighted. Finally, we consider that DEP has the potential of becoming a cytometric method for large‐scale sorting and characterization of cells.

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“…The work in [1,2] proposed a microfluidic device for the separation of abnormal cells from blood. The microdevice is designed by interdigitating electrodes on the bottom surface of the microchannel.…”

Section: Related Work and Backgroundmentioning

confidence: 99%

“…In the design of separation devices for microentities, electrodes are integrated into the microchannel to subject microentities with DEP force normal to the direction of flow. As a result, microentities within the medium are pushed away/to from the electrodes [1,2].…”

Section: Introductionmentioning

confidence: 99%

Design and Verification of a Blood Cell Separation Microfluidic Device

2017

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Abstract-Blood cell separation microdevices are designed in biomedical engineering for the separation of particular cells from blood, such as cancer cells. The movement of blood microentities, especially abnormal ones, in a continuous flow microfluidic device is controlled by several forces. Therefore, understanding and guiding the movement of these microentities is a challenging problem. These cells are subject to different types of forces that result from natural or external effects. These forces include that due to gravity, virtual mass, buoyancy, dielectrophoresis, and inertia. Therefore, these are to be accounted for in any design or implementation of a system. In this paper we use formal analysis of a separation microdevice to model and verify the microenetit's movement and behavior at high level of abstraction while considering different types of forces. The dynamic behavior of the microentity can be modeled as a Markovian decision process to predict the trajectory of the same. This model can provide probabilistic analysis for the microentity movement in the microdevice under the effect of different types of forces.

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Lab-on-chip for liquid biopsy (LoC-LB) based on dielectrophoresis

Cited by 35 publications

References 18 publications

Micro-Pattern of Graphene Oxide Films Using Metal Bonding

Micro-Pattern of Graphene Oxide Films Using Metal Bonding

Highlighting the uniqueness in dielectrophoretic enrichment of circulating tumor cells

Design and Verification of a Blood Cell Separation Microfluidic Device

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