Labdane diterpenes in Curcuma mangga rhizomes inhibit lipid peroxidation, cyclooxygenase enzymes and human tumour cell proliferation

Curcuma mangga

“…Chemical investigation from the hexane and ethyl acetate fractions of the methanol rhizome extract resulted in the isolation of seven pure compounds, namely, ( E )-labda-8(17),12-dien-15,16-dial, ( E )-15,16-bisnor-labda-8(17),11-dien-13-on, zerumin A, β-sitosterol, curcumin, demethoxycurcumin and bis -demethoxycurcumin (Malek et al 2011 ). The methanol and ethyl acetate rhizome extracts yielded bioactive labdane diterpenoids labda-8(17)-12-diene-15,16-dial ( 1 ), calcaratarin A ( 2 ), communic acid ( 3 ), copallic acid ( 4 ), zerumin B ( 5 ), 14,15,16-trinor-labdan-8,12-diol ( 6 ) and two decalins ( 7 -8 ) (Liu and Nair 2011 ).…”

“…Leaves of C. mangga had total phenolic content (TPC) of 275 mg GAE/100 g and ascorbic acid equivalent antioxidant capacity (AEAC) of 118 mg AA/100 g and rhizome TPC of 112 mg GAE/100 g and AEAC of 33 mg AA/100 g (Chan et al 2008 ). Compounds purified from the methanol and ethyl acetate rhizome extracts, namely, bioactive labdane diterpenoids labda-8(17)-12-diene-15,16-dial ( 1 ), calcaratarin A ( 2 ), communic acid ( 3 ), copallic acid ( 4 ), zerumin B ( 5 ), 14,15,16-trinor-labdan-8,12-diol ( 6 ) and two decalins ( 7 -8 ), inhibited lipid peroxidation (LPO) by 12-45 % at 25 μg/ml (Liu and Nair 2011 ).…”

“…Compounds purifi ed from the methanol and ethyl acetate rhizome extracts, namely, bioactive labdane diterpenoids labda-8(17)-12-diene-15,16-dial ( 1 ), calcaratarin A ( 2 ), communic acid ( 3 ), copallic acid ( 4 ), zerumin B ( 5 ), 14,15,16-trinorlabdan-8,12-diol ( 6 ) and two decalins ( 7 -8 ), exhibited anti-infl ammatory activity in-vitro (Liu and Nair 2011 ). In cyclooxygenase enzyme (COX-1 and COX-2) inhibitory assays, compounds 1 -4 showed selective COX-2 enzyme inhibition by 39-55 %, whereas 5 -8 showed selectivity to COX-1 between 45 and 53 %.…”

“…Compounds ( E )-labda-8(17),12-dien-15,16-dial and zerumin A from the fractions exhibited high cytotoxic effects against all six selected cancer cell lines, while compound ( E )-15,16-bisnor-labda-8(17),11-dien-13-on showed no antiproliferative activity on the tested cell lines. Compounds purifi ed from the methanol and ethyl acetate rhizome extracts, namely, bioactive labdane diterpenoids labda-8(17)-12-diene-15,16-dial (1), calcaratarin A (2), communic acid (3), copallic acid (4), zerumin B ( 5 ), 14,15,16-trinor-labdan-8,12-diol (6) and two decalins (7 -8) at 25 μg/ml, showed some degree of inhibition against NCI-H460 (lung), , AGS (gastric), SF-268 (CNS) and MCF-7 (breast) human tumour cell progression (Liu and Nair 2011 ).…”

Curcuma longa and Curcuma mangga leaves exhibit functional food property

“…The inhibitory effects of test samples on COX-1 and -2 were measured by monitoring the initial rate of O 2 uptake using an oxygen electrode (Instech laboratories, Plymouth Meeting, PA) attached to a biological oxygen monitor (Yellow Spring Instrument, Inc., Yellow Spring, OH) at 37°C as per published procedure (Liu & Nair, 2011;Seeram et al, 2003). The extracts and compounds (1-9) were tested at 100 and 25 lg/mL concentrations, respectively.…”