Labdane‐type diterpenes from Hedychium gardnerianum with potent cytotoxicity against human small cell lung cancer cells

Abstract

Seven labdane-type diterpenes, coronarin E, coronarin A, yunnancoronarin A, yunnancoronarin B, hedyforrestin B, villosin, and hedyforrestin C were isolated from the rhizome of Hedychium gardnerianum and evaluated for cytotoxic activity against human small cell lung cancer (NCI-H187) and non-cancerous Vero cells. The results showed that villosin exhibited potent cytotoxic activity with IC(50) of 0.40 microM, which was higher than that of the drug ellipticine (IC(50) 1.79 microM). Moreover, ellipticine was very toxic to Vero cells (IC(50) 7.47 microM) whereas the toxicity of villosin was undetectable at concentration lower than 166.42 microM. The results have indicated that the lactone ring is essential for high cytotoxic activity and that the presence of a hydroxyl group at the 6 or 7 position causes decrease in activity. The very high cytotoxicity against the NCI-H187 cells and the exceptionally high selectivity index (>416) of villosin suggested that this compound may be used as a potential lead molecule for antitumor therapeutic development.

Phytochemical Analysis, Antioxidant, Antibacterial, Cytotoxic, and Enzyme Inhibitory Activities of Hedychium flavum Rhizome

“…According to previous studies, the coronarin E, as the most predominant constituent in H. flavum EO, had higher cytotoxicity to human tumor cell lines (NCI-H187, HL-60, THP-1, and A-549) (IC 50 : 31.21-53.26 mM) compared to noncancerous Vero cell (IC 50 : 150.45 mM), with selective cytotoxicity against tumor cells (Reddy et al, 2009;Kumrit et al, 2010). The cytotoxic properties of other main components in EO, such as 1,8cineole, b-pinene, a-pinene, E-nerolidol, linalool, and acurcumene, have been demonstrated in previous reports (Hassan et al, 2010;Shin and Lee, 2013;Dall'Acqua et al, 2017;Salehi et al, 2019).…”

Manool, a Salvia officinalis diterpene, induces selective cytotoxicity in cancer cells

“…Two labdane-type diterpenoids, yunnancoronarin A and B, showed interesting IC 50 values (0.27 and 0.69 lg/mL) against lung adenocarcinoma (A549) and leukemia (K562) cell lines, respectively. Kumrit et al (2010) evaluated the cytotoxic activity of villosin and ellipticine, labdane-type diterpenoids, against human small cell lung cancer (NCI-H187) and non-cancerous (Vero cells) cell lines. The results showed that villosin exhibited potent cytotoxic activity (IC 50 of 0.12 lg/ mL) against NCI-H187, while the IC 50 for ellipticine was 0.44 lg/mL.…”

The Ginger Prophecy; A Review of the Underexplored Genus, Hedychium against Cancer

“…Arruda et al [126] studied the essential oils of H. gardnerianum, which exhibited antiacetylcholinesterase and antioxidant activity making the plant potentially be useful as an adjuvant in treatment of Alzheimer's disease. Kumrit et al [127] reported isolation of 7 labdane diterpenes from this plant, which showed cytotoxic activity. They isolated coronarin E, coronarin A, yunnancoronarin A, yunnancoronarin B, hedyforrestin B, villosin and hedyforrestin C from the rhizome of this plant and tested the cytotoxic activity in vitro on lung cancer (NCI-H187) and non-cancerous Vero cells.…”