Label-Free Acetylcholine Image Sensor Based on Charge Transfer Technology for Biological Phenomenon Tracking

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chip with LSI, CMOS and MEMS process, SOI material and devices, VLS probe chip, pH image sensor The sensor chips developed in Toyohashi University of Technology and the facility called the "LSI factory" since 1979 were reviewed. The original and unique devices were fabricated using not only the standard Si-LSI process and materials, but also the MEMS process and materials. Researchers including students can fabricate their own devices by themselves, which could lead to the production of original prototype devices using special processes and materials together with the CMOS process. Demonstrating the devices is more persuasive than theoretically explaining them in this manuscript. Aside from its educational use, this explains the existence of the LSI factory in Toyohashi University.

Section: Ion Image Sensorsmentioning

confidence: 99%

Ideal Sensor Chips —A Smart Microsensor Chip with LSI and MEMS Process and Materials—

Ishida¹

2018

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“…The number of pixels and their density have been increased: 32 × 32 pixels with a pitch of 130 μm, (29) 128 × 128 pixels with a pitch of 23 μm, (30) and 1024 × 1024 pixels with a pitch of 12.1 μm (31) have been reported. In addition to pH, the concentration of other ions such as K + (29) and Na + (32) and biomolecules such as acetylcholine (33,34) have been measured by modifying of the sensor area. A multimodal sensor, which simultaneously measures an optical image and a pH image, was also proposed.…”

Section: Ccd/cmos-based Ph Imaging Sensormentioning

confidence: 99%

“…The visualization of acetylcholine with a CCD-based pH imaging sensor has also been reported. (33,34,77) …”

Section: Enzymatic Reactionmentioning

confidence: 99%

Development and Bio-imaging Applications of a Chemical Imaging Sensor

2016

A chemical imaging sensor is a field-effect-based semiconductor sensor, capable of label-free imaging of ion distributions in a solution. The sensor has attracted much interest owing to its simple structure and diverse fields of application of bioimaging. In this article, the principle and development of the chemical imaging sensor are summarized, compared with those of other labelfree imaging sensors. Bio-imaging applications of the chemical imaging sensor to ion diffusion, enzymatic reaction, and metabolic activities of microorganisms are also reviewed.

“…Various neurotransmitters and biomolecules have been detected by potentiometric techniques including ion-sensitive field-effect transistors (8) and charge-transfer-type potentiometric sensors. (10) For example, the detection of acetylcholine (ACh), (11)(12)(13) glutamate (Glu), (14) and glucose (12,15) has been reported, and they were detected as pH changed with enzymatic reactions. In biological environments, however, it is necessary to use pH buffers, which suppress pH changes caused by enzymatic reactions, resulting in a reduction in the magnitude of output signal.…”

Section: Introductionmentioning

confidence: 99%

H2O2 Detection by Redox-based Potentiometric Sensors under Biological Environments

Iwata¹,

Mizutani²,

Okumura³

et al. 2018

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Hydrogen peroxide (H 2 O 2) is an important target material for detecting biomolecules including acetylcholine (ACh), glutamate (Glu), and glucose. In this study, we report on H 2 O 2 detection under biological environments based on the redox reaction. The redox potential change caused by the reaction between the electron mediators of ferrocenes and H 2 O 2 catalyzed by horseradish peroxidase (HRP) was measured using a gold electrode connected to a source follower circuit. The mediators were either dissolved in sample solutions using ferrocenyl methanol (FcMeOH) or immobilized on the sensor surface in the form of 11-ferrocenyl-1undecanethiol (11-FUT). H 2 O 2 detection under biological environments was demonstrated in both samples. The overall outputs in the 11-FUT-immmobilzed electrodes were lower than those in the samples with dissolved FcMeOH. The detection range of H 2 O 2 was from 10 −5 to 10 −3 M for the samples with dissolved FcMeOH, while it was from 10 −4 to 10 −2 M for the 11-FUT-immobilized electrodes. It was suggested that the oxidation of the mediators by H 2 O 2 insufficiently took place in the 11-FUT-immobilized electrodes, leading to the lower outputs.