Label-free affinity biosensor arrays: novel technology for molecular diagnostics

Johnson, Steven; Krauss, Thomas F.

doi:10.1080/17434440.2017.1283982

Cited by 13 publications

(8 citation statements)

References 16 publications

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“…In addition, they do not alter the main electrical and optical properties of the TCO, as we have demonstrated in our previous works . Electrografting allows for the chemical modification of surfaces with a high spatial confinement, as surface activation can take place in a very restricted area . This can be done alternatively with direct‐write, highly sophisticated lithographic tools, such as the set of methods belonging to scanning probe lithography, which in turn are extremely time‐consuming …”

Section: Introductionmentioning

confidence: 89%

A Combined Electrochemical‐Microfluidic Strategy for the Microscale‐Sized Selective Modification of Transparent Conductive Oxides

Lamberti

Salmaso

Zambon

et al. 2017

Adv Materials Inter

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Surface chemical functionalization of transparent conductive oxides (TCOs) is helpful for a wide range of technological applications, ranging from solar cells to biomedical devices, as it allows to tune the electrical, optical, and morphological properties of TCOs toward the desired goal. The electrochemical grafting technique is a surface modification methodology affording robust coatings with tuneable properties and has the potential to be exploited for modifying TCO surfaces. However, due to technical limitations, like the use of a 3‐electrode cell and the need for low pH‐solutions, this approach has not been recurrently applied. Here a novel electrochemical‐microfluidic combined methodology is used where the use of a microchannel drives the spatially controlled covalent grafting of reagents on a TCO surface. To corroborate the validity of this approach in producing more complex chemical structures localized on selected microscale‐sized areas, where a first electrochemical grafting step takes place, an electrochemical glucose biosensor is realized through a layer‐by‐layer approach that shows a remarkable limit of detection in the micromolar concentration range. The sensing mechanism is based on an efficient electron transfer from glucose to the functionalized TCO surface. Biosensor performance is conveniently tuned by acting on the number of enzymatic units loaded onto the biosensor‐tree.

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Section: Introductionmentioning

confidence: 89%

A Combined Electrochemical‐Microfluidic Strategy for the Microscale‐Sized Selective Modification of Transparent Conductive Oxides

Lamberti

Salmaso

Zambon

et al. 2017

Adv Materials Inter

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“…or radioimmuno assay (RIA), were introduced to the analytical field over 40 years ago and are currently using as gold standards for many applications, especially biomarkers detection or protein analysis (Johnson and Krauss, 2017). with reporter system such as the enzyme horseradish peroxidase (HRP).…”

Section: Labelled Vs Label-free Sensing Platformmentioning

confidence: 99%

One-step gold nanoparticle size-shift assay using synthetic binding proteins and dynamic light scattering

Mahatnirunkul

Tomlinson

McPherson

et al. 2022

Sensors and Actuators B: Chemical

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“…For this goal, various labeling strategies are used to amplify the detection signal in biosensors technology. Labeling may involve avidin-biotin conjugation along with redox enzymes, as well as covalent attachment, intercalation, or the electrostatic interaction of small molecules/particles/ions with the biorecognition elements, which are responsible for generating the electrochemical signal [71].…”

Section: Nanobioengineered Electrochemical Biosensorsmentioning

confidence: 99%

“…In contrast to conventional label-based biosensors, label-free biosensors are based solely on the measurement of changes of the electrical signal at the electrode surface when the interaction of the target analyte and the biological recognition element occurs [72]. Label-free biosensors directly transduce a molecular binding event into a physically measurable quantity, i.e., without the need for an additional antibody, enzymatic, or electroactive label, or any other amplification strategy, to provide a response that is proportional to the concentration of bound molecules [71]. Transduction is commonly achieved by measuring a physical property change, such for example, the charge transfer resistance (R ct ) by EIS [73].…”

Section: Nanobioengineered Electrochemical Biosensorsmentioning

confidence: 99%

Electrochemical Biosensors for Determination of Colorectal Tumor Biomarkers

et al. 2020

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The accurate determination of specific tumor markers associated with cancer with non-invasive or minimally invasive procedures is the most promising approach to improve the long-term survival of cancer patients and fight against the high incidence and mortality of this disease. Quantification of biomarkers at different stages of the disease can lead to an appropriate and instantaneous therapeutic action. In this context, the determination of biomarkers by electrochemical biosensors is at the forefront of cancer diagnosis research because of their unique features such as their versatility, fast response, accurate quantification, and amenability for multiplexing and miniaturization. In this review, after briefly discussing the relevant aspects and current challenges in the determination of colorectal tumor markers, it will critically summarize the development of electrochemical biosensors to date to this aim, highlighting the enormous potential of these devices to be incorporated into the clinical practice. Finally, it will focus on the remaining challenges and opportunities to bring electrochemical biosensors to the point-of-care testing.

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Label-free affinity biosensor arrays: novel technology for molecular diagnostics

Cited by 13 publications

References 16 publications

A Combined Electrochemical‐Microfluidic Strategy for the Microscale‐Sized Selective Modification of Transparent Conductive Oxides

A Combined Electrochemical‐Microfluidic Strategy for the Microscale‐Sized Selective Modification of Transparent Conductive Oxides

One-step gold nanoparticle size-shift assay using synthetic binding proteins and dynamic light scattering

Electrochemical Biosensors for Determination of Colorectal Tumor Biomarkers

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