Label-Free and Real-Time Optical Detection of Affinity Binding of the Antibody on Adherent Live Cells
Xiaoyi Li,
Shiming Wu,
Zhihao Feng
et al.
Abstract:Oblique-incidence reflectivity difference (OIRD) is a novel real-time, label-free, and nondestructive optical detection method and exhibits encouraging application in the detection of antibody/DNA microarrays. In this study, for the first time, an OIRD label-free immunoassay was achieved by using adherent live cells as the probe. The cells were cultured on glass cells, and the affinity binding of antibodies targeted on the HLA class I antigen of the cell surface was detected with an OIRD. The results show that… Show more
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