Label-free and sensitive faradic impedance aptasensor for the determination of lysozyme based on target-induced aptamer displacement

Peng, Yage; Zhang, Dongdong; Li, Yan; Qi, Honglan; Gao, Qiang; Zhang, Chengxiao

doi:10.1016/j.bios.2009.06.001

Cited by 91 publications

(40 citation statements)

References 43 publications

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“…Next to these approaches, in recent years, a few aptamer-based analytical methods have been developed towards lysozyme recognition and detection [47,[55][56][57][58][59][60][61][62][63]. Aptamers are small single-stranded oligonucleotides (DNA or RNA) that are selected in vitro to bind with high affinity and specificity any selected target of choice, from small-sized, such as metal ions [64], to large ones, such as cells [65].…”

Section: Quantification Methodsmentioning

confidence: 99%

“…The gain in sensitivity comes with a cost in complexity, price per assay and inconvenience associated with limited enzyme stability; these should be all considered before choosing the "right" aptasensor design for a particular application. resulting in a conformational change of methylene blue-tagged DNA into a hairpin structure; this brings methylene blue closer to the electrode surface, leading to an increase of its signal (signal-on sensor) [90] 4. displacement of LBA from its complex with DNA upon lysozyme addition (signal-off sensor) [46,62,95] 5. electrochemical stripping of lysozyme/quantum-dots complex [94] Although simple, practical and label-free, these aptasensors lack the required sensitivity for more demanding applications, such as the detection of lysozyme in biological samples or of trace amounts of lysozyme in wine. Moving forward from the above direct assay, a sandwich between the surface linked aptamer, the captured lysozyme and a biotinylated anti-lysozyme antibody has been recently proposed to enhance the sensitivity of the sensor ( Figure 4C) [44].…”

Section: Electrochemical Assay Formats: Direct Sandwich and Competitmentioning

confidence: 99%

“…The sensing principles applied rely on: displacement of LBA from its methylene blue-tagged DNA complex in the presence of lysozyme, resulting in a conformational change of methylene blue-tagged DNA into a hairpin structure; this brings methylene blue closer to the electrode surface, leading to an increase of its signal (signal-on sensor) [90] 4. displacement of LBA from its complex with DNA upon lysozyme addition (signal-off sensor) [ desorption of lysozyme aptamer from rGO/Orange II-modified GCE, reversing the blocking effect and reestablishing efficient electron transfer from graphene-adsorbed aromatic dye Orange II [85] Competitive detection schemes are typically used with regenerable sensors, where in order to revert to the characteristics of the original interface, the aptasensor needs simply to be re-incubated in fresh aptamer solution at the end of each measurement [62,89]. An ingenious detection strategy for multiple protein detection relied on immobilization of dabcyl-labeled-aptamer-modified metal nanoparticles (DLAPs) on a β-cyclodextrin-modified electrode by host-guest affinity [89].…”

Section: Electrochemical Assay Formats: Direct Sandwich and Competitmentioning

confidence: 99%

“…click chemistry between azide-modified gold particles and alkyne-terminated aptamers ( Figure 2D) [60] 5. electrostatic interactions between the negatively-charged phosphate backbone of the aptamer and positively-charged materials ( Figure 2E), such as polypyrrole, Fe 2 O 3 and ferrocene-appended poly(ethyleneimine) (Fc-PEI) in a layer-by-layer approach or amine-rich films of plasma-polymerized propargylamine in a Cu 2 O@rGO@PpPG-modified gold electrode [74,78,84,88] 6. affinity binding based on biotin-avidin [80] or host-guest interactions [89] (Figure 2F) 7. hybridization to a partially complementary DNA strand, previously immobilized on the electrode surface ( Figure 2G) [96] Chemosensors 2016, 4, 10 7 of 21 The majority of electrochemical aptasensors for lysozyme used thiolated aptamers on gold electrode, that is following Strategy (C) [46,62,87,91,94]. To increase the aptamer loading, a popular approach relies on modifying the electrodes with Au nanoparticles [47,76].…”

mentioning

confidence: 99%

“…The majority of electrochemical aptasensors for lysozyme used thiolated aptamers on gold electrode, that is following Strategy (C) [46,62,87,91,94]. To increase the aptamer loading, a popular approach relies on modifying the electrodes with Au nanoparticles [47,76].…”

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confidence: 99%

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Aptamer-Based Electrochemical Sensing of Lysozyme

Vasilescu

Wang

et al. 2016

Chemosensors

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Protein analysis and quantification are required daily by thousands of laboratories worldwide for activities ranging from protein characterization to clinical diagnostics. Multiple factors have to be considered when selecting the best detection and quantification assay, including the amount of protein available, its concentration, the presence of interfering molecules, as well as costs and rapidity. This is also the case for lysozyme, a 14.3-kDa protein ubiquitously present in many organisms, that has been identified with a variety of functions: antibacterial activity, a biomarker of several serious medical conditions, a potential allergen in foods or a model of amyloid-type protein aggregation. Since the design of the first lysozyme aptamer in 2001, lysozyme became one of the most intensively-investigated biological target analytes for the design of novel biosensing concepts, particularly with regards to electrochemical aptasensors. In this review, we discuss the state of the art of aptamer-based electrochemical sensing of lysozyme, with emphasis on sensing in serum and real samples.

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Section: Quantification Methodsmentioning

confidence: 99%

Section: Electrochemical Assay Formats: Direct Sandwich and Competitmentioning

confidence: 99%

Section: Electrochemical Assay Formats: Direct Sandwich and Competitmentioning

confidence: 99%

mentioning

confidence: 99%

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confidence: 99%

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Aptamer-Based Electrochemical Sensing of Lysozyme

Vasilescu

Wang

et al. 2016

Chemosensors

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Aptamer/Nanoparticle‐Based Sensitive, Multiplexed Electronic Coding of Proteins and Small Biomolecules through a Backfilling Strategy

Qian

Xiang

Zhang

et al. 2010

Chemistry A European J

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The development of sensitive and selective bioassays capable of monitoring important biomolecules in a multiplexed fashion is a major goal in biosensing. [1][2][3][4][5] In many cases, measurement of a single biomarker is not sufficient to diagnose and follow the disease status. Rather, simultaneous measurement of a panel of biomarkers is necessary to reach this goal; this has led to increasing research on multiplexed analysis of biomolecules. Various multiplexed detection schemes for DNA or proteins based on labels with distinct readouts, such as optically [6][7][8][9][10][11] or electrochemically [12][13][14][15] encoded quantum dots, multisegment nanowires, [16][17][18][19] dye-embedded microspheres, [20][21][22][23][24] and biobarcode nanoparticles, [25,26] have thus been demonstrated. Although multiplexed analysis of DNA or proteins is well developed, simultaneous monitoring of analytes with significant difference in sizes, for instance, small biomolecules and proteins, has rarely been exploited and remains a challenge mainly due to the lack of double-probe sandwich assay formats for the low-molecular-weight analytes.Herein, we report, for the first time, a novel aptamer/ nanoparticle-based backfilling strategy for one-spot simultaneous detection of proteins and small biomolecules, employing lysozyme and adenosine triphosphate (ATP) as the model target analytes. Our approach relies on target-induced release of aptamers from the DNA duplexes on a sensing surface, followed by backfilling hybridization of the resulting single-stranded DNA molecules with aptamers conjugated to the electrochemically encoded nanoparticles. Subsequent unique electrochemical (EC) signatures of the acid-dissolved nanoparticles at distinct potential positions with well-resolved peaks thus reflect the identities and concentrations of lysozyme and ATP. Compared with previously reported multiplexed detection schemes, our new analysis route for proteins and small biomolecules offers three clear advantages. First, simultaneous determination of biomolecules with distinct molecular weights (lysozyme, 14.3 kDa versus ATP, 507.2 Da) is achieved for the first time with electrochemically encoded nanoparticle tags. Second, in contrast to other common "signal off" assay formats for lysozyme [27][28][29] and ATP based on target-induced displacement [30][31][32] or conformational changes of the corresponding aptamers, [33][34][35][36] our multiplexed method is a "signal on" configuration, which exhibits improved sensitivity.[35] Third, the analytical signal amplification by the nanoparticle labels composed of a large number of metal ions and the high sensitivity of the voltammetric stripping detection lead to lowconcentration determination of the target analytes. The combination of these advantages facilitates the detection of biomolecules with distinct sizes in a multiplexed and sensitive manner.Our multiplexed analysis approach for proteins and small molecules based on the aptamer/nanoparticle bioconjugates and the backfilling strategy, illustrate...

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Impedimetric Detection of Hairpin Ribozyme Activity

et al. 2010

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Electrochemical impedance spectroscopy is successfully utilized for label-free monitoring of the cleavage reaction of a RNA substrate by a complementary hairpin ribozyme. The formation of the RNA substrate/ribozyme complex is increasing the negative charge at the interface and is modulating the kinetic of the redox conversion of a negatively charged redox mediator (e.g. [Fe(CN) 6 ] 3À/4À ) hence leading to an increase in the charge transfer resistance. Upon addition of bivalent cations such as Mg 2 + ions the conformation of the ribozyme is changed and the catalytic cleavage of the RNA is monitored by a decrease of the charge transfer resistance.

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Label-free and sensitive faradic impedance aptasensor for the determination of lysozyme based on target-induced aptamer displacement

Cited by 91 publications

References 43 publications

Aptamer-Based Electrochemical Sensing of Lysozyme

Aptamer-Based Electrochemical Sensing of Lysozyme

Aptamer/Nanoparticle‐Based Sensitive, Multiplexed Electronic Coding of Proteins and Small Biomolecules through a Backfilling Strategy

Impedimetric Detection of Hairpin Ribozyme Activity

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