Label-free based comparative proteomic analysis of whey proteins between different milk yields of Dezhou donkey

Zhang, Xinhao; Li, Haijing; Yu, Jie; Zhou, Xiangshan; Ji, Chuanliang; Wu, Shuaishuai; Chen, Yuanqing; Liu, Jinghui; Zhao, Fuwei

doi:10.1016/j.bbrc.2018.11.130

Cited by 18 publications

(12 citation statements)

References 35 publications

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“…Feed intake (DMI) and milking yield (MY, kg/day) were recorded daily. Milk yield was calculated as follows: estimated milk yield (EMY, kg/day) = milking yield (kg/day) × 4 [ 25 ]; solids-corrected milk (SCM, kg/day) = {(12.3 × milk fat (%) content of non-standard milk +6.56 × solid-not-fat (%) content of non-standard milk−0.0752)} × Estimated milk yield (kg/day) [ 26 ], energy-corrected milk (ECM, kg/day) = 0.327 × EMY (kg/day) + 12.95 × fat (kg/day) + 7.65 × protein (kg/day) [ 27 ]. Feed conversion rate was expressed as follows: EMY (kg/day)/DMI (kg/day), SCM (kg/d)/DMI (kg/day) and ECM (kg/day)/DMI (kg/day).…”

Section: Methodsmentioning

confidence: 99%

Effect of Varying Dietary Crude Protein Level on Milk Production, Nutrient Digestibility, and Serum Metabolites by Lactating Donkeys

Yue

Tong

et al. 2022

Animals

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Donkey milk is considered as a functional food due to its high levels of whey protein, and can be used in newborn nutrition, due to the nutritional similarities with human milk and its hypoallergenic property. However, donkey milk yield is very low and little is known about improving donkey milk yield by nutrition manipulation. The effect of dietary crude protein (CP) levels on milk production, nutrient digestibility, and serum metabolites was investigated in the current study. Twenty-four lactating donkeys were randomly assigned to one of the following three CP content diets: 15.3% (HP), 14.2% (MP), and 13.1% (LP) of dry matter, respectively. The experiment lasted for 10 weeks, with the first two weeks being used for adaptation. The results showed that milk yield and yields of protein, lactose, solid-not-fat, total solid, and contents of protein, total solid and milk urea nitrogen in the HP and MP groups were higher than the LP group. No significant changes were observed in dry-matter intake, contents of milk fat, lactose or solid-not-fat. The feed conversion ratio, milk protein synthesis efficiency, and the digestibility of dry matter, crude protein, ether extract, acid detergent fiber, neutral detergent fiber, calcium and phosphorous in the HP and MP groups were greater than the LP group. Serum total protein, albumin and urea nitrogen concentrations decreased, while concentrations of non-esterified fatty acids and β-hydroxybutyrate increased in the LP group compared with the HP and MP groups. In conclusion, the diet containing 14.2% CP supplied an adequate amount of protein for improving milk production in lactating donkeys, but milk production was not further increased by feeding the donkeys more than 14.2% CP.

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Section: Methodsmentioning

confidence: 99%

Effect of Varying Dietary Crude Protein Level on Milk Production, Nutrient Digestibility, and Serum Metabolites by Lactating Donkeys

Yue

Tong

et al. 2022

Animals

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“…The identification of HSP90AB1 using the methods developed here is feasible as it has been found among the secreted proteins that are components of extracellular vesicles (Hoshino et al, 2020). Furthermore, HSP90AB1 has been identified as a component protein in the whey of several species besides sheep, (Johnston et al, 2018;Zhang et al, 2019) and has been reported among the identified proteins in proteomics studies of ovine milk (Addis et al, 2013;Pisanu et al, 2015;Zhang et al, 2020). Regarding its levels in mastitic conditions, there are two studies that report its transcription in bovine mammary gland to be upregulated after infection by E. coli (Chen et al, 2015), and a proteomics study that reports it to be elevated in milk from goats with mastitis (Pisanu et al, 2020b).…”

Section: Identification Of Potential Markers Related To Early Intrama...mentioning

confidence: 99%

Utilization of 2D and 3D cell cultures for the modelling of intramammary infection in sheep

et al. 2022

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Ovine mastitis is defined as the inflammation of the sheep udder, most commonly caused in response to intramammary infections. Based on the occurrence of clinical signs, mastitis is characterized as either clinical or subclinical (SCM). The impact of ovine SCM on the overall sustainability of dairy sheep farms has been substantially documented underpinning the significance of efficient diagnosis. Although SCM can be detected in cows, the performance and the validity of the methods used do not transfer in dairy sheep. This fact challenges the development of evidence-based ovine udder health management protocols and renders the detection and control of ovine mastitis rather problematic. Currently, cell culture-based models are being successfully used in biomedical studies and have also been effectively used in the case of bovine mastitis. The objective of the present study was to culture ovine primary mammary cells for the development of 2D and 3D cell culture-based models for the study of ovine mammary gland and to focus on the first stages of the intramammary infection by common mastitis-inducing pathogens. Cells were infected by E. coli and S. aureus mimicking the first stages of natural intramammary infections. The secreted proteins were subjected to mass-spectrometry resulting in the identification of a total of 79 distinct proteins. Among those, several had already been identified in healthy or mastitic milk, while others had not been previously detected for in the ovine mammary secretome. Our results suggest that the development of cell-based models for studying specific stages of intramammary infection has the potential to be beneficial for the udder health management in dairy sheep.

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“…Comparative analysis of whey proteins in donkey colostrum and mature milk was also performed using quantitative proteomics ( 15 ). Our previous study analyzed the difference in proteomic levels of DM whey proteins with different yield by label-free mass spectrometry ( 16 ). These studies provided useful information regarding the DM whey protein components; however, there are relatively few studies on the difference of whey protein between DM and breast milk based on quantitative proteomics.…”

Section: Introductionmentioning

confidence: 99%

Comparative Whey Proteome Profiling of Donkey Milk With Human and Cow Milk

Zhang

Jiang²,

Ji³

et al. 2022

Front. Nutr.

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Donkey milk (DM), similar to human milk (HM) in chemical composition, has been suggested as the best potential hypoallergenic replacement diet for babies suffering from Cow milk (CM) protein allergy. In order to better understand DM protein, many studies based on proteomic have been performed. In this study, the label-free quantitative proteomic approach was conducted to quantitatively identify the differentially expressed whey proteins (DEPs) in DM vs. HM group and DM vs. CM group. In total, 241 and 365 DEPs were found in these two groups, respectively. Bioinformatics analysis of DEPs showed that the majority of DEPs participated in the lipoprotein metabolic process, regulation of cytokine production, chemical homeostasis, and catabolic process. The Kyoto Encyclopedia of Gene and Genomes (KEGG) pathways analysis found that these DEPs mainly participated in an antigen processing, complement, and coagulation cascades. These results may provide valuable information in the composition of milk whey proteins in DM, HM, and CM, especially for low abundant components, and expand our knowledge of different biological functions between DM and HM or CM.

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Label-free based comparative proteomic analysis of whey proteins between different milk yields of Dezhou donkey

Cited by 18 publications

References 35 publications

Effect of Varying Dietary Crude Protein Level on Milk Production, Nutrient Digestibility, and Serum Metabolites by Lactating Donkeys

Effect of Varying Dietary Crude Protein Level on Milk Production, Nutrient Digestibility, and Serum Metabolites by Lactating Donkeys

Utilization of 2D and 3D cell cultures for the modelling of intramammary infection in sheep

Comparative Whey Proteome Profiling of Donkey Milk With Human and Cow Milk

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