Label-free based proteomics revealed the specific changes of muscle proteins in pike eel (Muraenesox cinereus) under cold stress

Cold-induced denaturation of muscle proteins in hairtail (Trichiurus lepturus) during storage: Physicochemical and label-free based proteomics analyses

“…2 C), which was beneficial for detection by MS/MS analysis. The digested peptides composed of fewer than 5 amino acids or more than 20 amino acids could not be effectively trapped by the MS/MS spectrometer, mainly owing to their low/high electric charges and incompatible MWs ( Yuan et al, 2022 ). These observations were in accordance with the digestion characteristics of trypsin in the muscle proteins.…”

“…Hairtail muscle proteins were extracted, digested, detected, and identified according to the method previously reported by Yuan et al (2022) . Briefly, the mashed samples (100 mg) were added to extraction buffer solutions (400 µL) containing 100 mmol/L Tris-HCl (4 °C, pH 8.0), 8.0 mol/L urea, 2.0 mol/L thiourea, 1 % (w/v) DTT, and 1 % (v/v) protease inhibitor.…”

“…Importantly, hairtail products, even under chilled or frozen conditions, are also susceptible to environmental stress (e.g., low temperature, light, and oxygen) due to the activity of spoilage bacteria, the abundance of proteases (e.g., trypsin-like protease, cathepsins, and calpains), and the high content of unsaturated FAs ( Hu et al, 2021 ). The deterioration of proteins and lipids in hairtail muscle tissues that unavoidably occurs during cold storage is undesirable ( Yuan, Chen, Benjakul, Sun, & Zhang, 2022 ), resulting in decreased flavor quality, texture degradation, drip loss, discoloration, and even the rancidity of hairtail muscle products ( Thanonkaew, Benjakul, Visessanguan, & Decker, 2008 ).…”

Bioinformatic Prediction and Characterization of Proteins in Porphyra dentata by Shotgun Proteomics

“…Catalytic activity (GO:0003824, 103) and binding (GO:0005488, 69) are the main molecular function. The KEGG pathway showed that most identified proteins are involved in amino acid ( 17 ), carbohydrate ( 24 ), and energy metabolism ( 23 ). Identified proteins also participate in protein translation ( 23 ), folding, sorting, degradation, and genetic information processing ( Figure 2B ).…”

“…This method has high sensitivity and wide application and is suitable for analyzing algal research with small molecular weight proteins (22). In a recent study, the label-free proteomic analysis revealed specific changes in muscle proteins in pike eel (Muraenesox cinereus) under cold stress and freshness-related proteins in sea bass (Lateolabrax japonicus) filets, during cold storage; however, no reports are available on protein changes at different harvest periods in seaweeds (23,24). This is the first study employing label-free shotgun proteomics to comprehensively identify the proteome of P. dentata during different harvest periods.…”