Label-free cancer markers detection by capacitance biochip

Carrara, Sandro; Bhalla, Vijayender; Stagni, Claudio; Benini, Luca; Ferretti, Anna M.; Valle, Francesco; Gallotta, Andrea; Riccò, B.; Samorı̀, Bruno

doi:10.1016/j.snb.2008.09.050

Cited by 60 publications

(42 citation statements)

References 48 publications

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“…The anchoring with the NH 2 groups is the most efficient binding. NH 2 groups are contained in Lysines and Arginines, which are widely spread over the entire antibody' surface [25]. So, randomly orientated antibodies are considered to estimate the size of antibodies layer.…”

Section: Memristors At the Nanoscalementioning

confidence: 99%

Memristive-biosensors: A new detection method by using nanofabricated memristors

Carrara

Sacchetto

Doucey

et al. 2012

Sensors and Actuators B: Chemical

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124

101

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Section: Memristors At the Nanoscalementioning

confidence: 99%

Memristive-biosensors: A new detection method by using nanofabricated memristors

Carrara

Sacchetto

Doucey

et al. 2012

Sensors and Actuators B: Chemical

Self Cite

124

101

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“…SAMs were formed onto template stripped gold (TSG) for AFM investigations, crystal quartz resonators for QCM investigations, and chips with interdigitated electrodes geometry for capacitance measurements. NH-terminated ssDNA probe molecules (Carrara et al, 2008) and antibodies (Lahiri et al, 1999) were immobilized onto the SAM monolayers by means of N-hydroxysuccinimide (NHS) and of EthylDimethyl-aminopropyl Carbodiimide (EDC) to form covalent bonds. Target DNA and antigen molecules were incubated following well established procedures.…”

Section: Surfaces Preparationmentioning

confidence: 99%

New insights for using self-assembly materials to improve the detection stability in label-free DNA-chip and immuno-sensors

Carrara¹,

Benini²,

Bhalla³

et al. 2009

Biosensors and Bioelectronics

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a b s t r a c tThis paper examines reliable advancements in low-cost DNA-and immuno-chips. Capacitance detection was successfully chosen to develop label-free bio-chips. Probe immobilization was rigorously investigated in order to obtain reliable capacitance measurements. Protein probes immobilized by using usual alkanethiols or thiolated ssDNA probes directly immobilized on gold do not allow sufficient stable capacitance measurements. New alkanethiols improved with ethylene-glycol function are shown in this paper to be more suitable materials for capacitive bio-chip development. Atomic Force Microscopy, Quartz Crystal Microbalance, and Capacitance Measurements were used to demonstrate that ethylene-glycol alkanethiols allow high time stability, smaller errors in detection, and improved ideal behaviour of the sensing surfaces. Measured capacitance is in the range of 8-11 nF/mm 2 for antibody layers and close to 6 nF/mm 2 for DNA probes. It is in the range of 10-12 nF/mm 2 and of 4-6 nF/mm 2 for antigen and DNA detection, respectively. The percentage error in detection is highly improved and it is in the range of 11-37% and of 0,23-0,82% for antigen and DNA, respectively. The reproducibility is also improved and it is close to 0,44% for single spot measurements on ethylene-glycol alkanethiols. A molecular theory attributing these improvements to water molecules strongly coordinated by ethylene-glycol functional groups and to solution ions not entering into probe films is finally proposed.

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“…Multiple voltammetric scans force ions inside the SWCNT matrix and assist the electrical double layer formation around the SWCNT walls, as shown by the fist scan in Figure 3 which differs from others. The drop casting of proteins further alters charge balance because new charges are introduced onto the surface [19]. Capacitance increases till equilibrated double layer prevents further charged species to reach the surface.…”

Section: B P450 2b4 Response Onto Swcnt Electrodesmentioning

confidence: 99%

Circuits design and nano-structured electrodes for drugs monitoring in personalized therapy

Carrara

Cavallini

Micheli

et al. 2008

2008 IEEE Biomedical Circuits and Systems Conference

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Abstract-Drug development and personalized therapy require accurate and frequent monitoring of the metabolic response by living tissues to treatments. In case of high risk side effects, e.g. therapies with interfering anti-cancer molecule cocktails, direct monitoring of the patient's drugs metabolism is essential as the metabolic pathways efficacy is highly variable on a patient-bypatient basis. Currently, there are no fully mature point-of-care bio-sensing systems for drugs metabolism monitoring directly in blood. The aim of the paper is to investigate solutions to develop point-of-care systems for drugs monitoring in personalized therapy. P450 enzymes are the considered probe molecules as they are key proteins directly involved in drugs metabolism of humans. Sensitivity improvement is ensured by means of enzyme integration onto electrodes structured with carbon nanotubes. Component Off-The-Shelf (COTS) based circuits design is investigated toward bio-chip development. Results show that the proposed circuitry is suitable for the aim and confirm that nanotubes are detection enhancers.

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Label-free cancer markers detection by capacitance biochip

Cited by 60 publications

References 48 publications

Memristive-biosensors: A new detection method by using nanofabricated memristors

Memristive-biosensors: A new detection method by using nanofabricated memristors

New insights for using self-assembly materials to improve the detection stability in label-free DNA-chip and immuno-sensors

Circuits design and nano-structured electrodes for drugs monitoring in personalized therapy

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