Label-free characterization of exosome via surface enhanced Raman spectroscopy for the early detection of pancreatic cancer

Carmicheal, Joseph; Hayashi, Chihiro; Huang, Xi; Liu, Lei; Lu, Yao; Krasnoslobodtsev, Alexey V.; Lushnikov, Alexander Y.; Kshirsagar, Prakash G.; Patel, Amie; Jain, Maneesh; Lyubchenko, Yuri L.; Lu, Yongfeng; Batra, Surinder K.; Kaur, Sukhwinder

doi:10.1016/j.nano.2018.11.008

Cited by 126 publications

(99 citation statements)

References 34 publications

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“…Examples of where chemometrics has been applied to SERS and related techniques include detection of cancers using PC-LDA and SVM [68][69][70][71], microbial pathogens by various discriminant and cluster analysis [72][73][74] and drug and their metabolites by PLS and ANNs [75][76][77].…”

Section: Multivariate Chemometricsmentioning

confidence: 99%

Enhancing Disease Diagnosis: Biomedical Applications of Surface-Enhanced Raman Scattering

et al. 2019

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Surface-enhanced Raman scattering (SERS) has recently gained increasing attention for the detection of trace quantities of biomolecules due to its excellent molecular specificity, ultrasensitivity, and quantitative multiplex ability. Specific single or multiple biomarkers in complex biological environments generate strong and distinct SERS spectral signals when they are in the vicinity of optically active nanoparticles (NPs). When multivariate chemometrics are applied to decipher underlying biomarker patterns, SERS provides qualitative and quantitative information on the inherent biochemical composition and properties that may be indicative of healthy or diseased states. Moreover, SERS allows for differentiation among many closely-related causative agents of diseases exhibiting similar symptoms to guide early prescription of appropriate, targeted and individualised therapeutics. This review provides an overview of recent progress made by the application of SERS in the diagnosis of cancers, microbial and respiratory infections. It is envisaged that recent technology development will help realise full benefits of SERS to gain deeper insights into the pathological pathways for various diseases at the molecular level.

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Section: Multivariate Chemometricsmentioning

confidence: 99%

Enhancing Disease Diagnosis: Biomedical Applications of Surface-Enhanced Raman Scattering

et al. 2019

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“…Following the analogous strategy, Carmicheal et al [33] designed a label-free substrate for early diagnose of pancreatic cancer based on the identification of exosomes extracted from serum of healthy donors (n = 10) and pancreatic cancer (PaC) patients (n = 10). Plasmonic material consists of gold slides, and positively charged AuNPs of 10 nm are deposited onto it to caption negatively charged exosomes from C18/HPAF, MiaPaCa, and HPDE pancreatic cancer cells.…”

Section: Label-free Sersmentioning

confidence: 99%

Cancer Diagnosis through SERS and Other Related Techniques

Blanco-Formoso

Alvarez-Puebla

2020

IJMS

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Cancer heterogeneity increasingly requires ultrasensitive techniques that allow early diagnosis for personalized treatment. In addition, they should preferably be non-invasive tools that do not damage surrounding tissues or contribute to body toxicity. In this context, liquid biopsy of biological samples such as urine, blood, or saliva represents an ideal approximation of what is happening in real time in the affected tissues. Plasmonic nanoparticles are emerging as an alternative or complement to current diagnostic techniques, being able to detect and quantify novel biomarkers such as specific peptides and proteins, microRNA, circulating tumor DNA and cells, and exosomes. Here, we review the latest ideas focusing on the use of plasmonic nanoparticles in coded and label-free surface-enhanced Raman scattering (SERS) spectroscopy. Moreover, surface plasmon resonance (SPR) spectroscopy, colorimetric assays, dynamic light scattering (DLS) spectroscopy, mass spectrometry or total internal reflection fluorescence (TIRF) microscopy among others are briefly examined in order to highlight the potential and versatility of plasmonics.

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“…SERS appears to be a promising potential method for the detection of miRNA in EVs [42,43]. It has the benefit of being fast and inexpensive to perform [28].…”

Section: Surface Enhanced Raman Spectroscopy (Sers) Biosensormentioning

confidence: 99%

Extracellular Vesicles in Diagnosis and Treatment of Pancreatic Cancer: Current State and Future Perspectives

Lane

Hoff

Cridebring

et al. 2020

Cancers

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Pancreatic cancer remains one of the deadliest diagnoses a patient can receive. One of the reasons for this lethality is that this malignancy is often detected very late due to a lack of symptoms during the early stages. In addition to the lack of symptoms, we currently do not have a reliable biomarker for screening. Carbohydrate antigen (CA) 19-9 has a sensitivity between 79% and 84% and a specificity of 82–90%, making it unreliable for early detection. Recently, there have been numerous studies on the use of extracellular vesicles (EVs) to detect pancreas cancer. This field has been rapidly expanding, with new methods and biomarkers being introduced regularly. This review provides a systematic update on the commonly used and promising methods used in the detection of EVs, biomarkers associated with EVs for early detection and prognosis, as well as studies looking at using EVs as therapeutics. The review ends with remarks about areas to focus on using EVs going forward.

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Label-free characterization of exosome via surface enhanced Raman spectroscopy for the early detection of pancreatic cancer

Cited by 126 publications

References 34 publications

Enhancing Disease Diagnosis: Biomedical Applications of Surface-Enhanced Raman Scattering

Enhancing Disease Diagnosis: Biomedical Applications of Surface-Enhanced Raman Scattering

Cancer Diagnosis through SERS and Other Related Techniques

Extracellular Vesicles in Diagnosis and Treatment of Pancreatic Cancer: Current State and Future Perspectives

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