Label-Free Characterization of Peptide–Lipid Interactions Using Immobilized Lipodisks

Hernández, Víctor Agmo; Reijmar, Karin; Edwards, Katarina

doi:10.1021/ac4012842

Cited by 12 publications

(20 citation statements)

References 42 publications

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“…A quartz crystal microbalance with dissipation monitoring (QCM-D) was used to study the interaction of VIP116 with lipodisks and lipid membrane. Silica or biotynilated gold sensors (Q-Sense, Gothenburg, Sweden) was mounted on a QCM-D E1 (Q-sense) instrument thermostatted at 21 • C. Frequency and dissipation data were collected at the 3rd, 5th, 7th, 9th, 11th and 13th overtones and the immobilized mass was calculated as previously described [22]. Matlab R2016b (The MathWorks, Natick, MA, USA) was used for performing the calculations.…”

Section: Qcm-dmentioning

confidence: 99%

Tumor-Targeted Delivery of the p53-Activating Peptide VIP116 with PEG-Stabilized Lipodisks

et al. 2020

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Stapled peptides targeting the interaction between p53 and its negative regulators MDM2 and MDM4 have exhibited great potential as anti-cancer drugs, albeit with room for improvement in formulation and tumor specificity. Lipid bilayer disks (lipodisks) have emerged as promising drug nanocarriers and can by attachment of targeting moieties be directed selectively towards tumor cells. Tumor-targeted delivery of stapled peptides by use of lipodisks may therefore increase the uptake in the tumors and limit toxicity in healthy tissue. Here, we utilized epidermal growth factor receptor (EGFR)-targeted lipodisks to deliver p53-activating stapled peptide VIP116 to EGFR-expressing tumor cells. We demonstrate that VIP116 can be stably formulated in lipodisks (maximum peptide/lipid molar ratio 0.11). In vitro cell studies verify specific binding of EGF-decorated lipodisks to tumor cells and confirm that targeted delivery of VIP116 significantly decreases tumor cell viability.

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Section: Qcm-dmentioning

confidence: 99%

Tumor-Targeted Delivery of the p53-Activating Peptide VIP116 with PEG-Stabilized Lipodisks

et al. 2020

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“…Other artificial lipid assemblies, including protein scaffold constructs ( i.e. nanodiscs) [68] and PEG-stablized lipodisks [69], may be capable of similar measurements described above, though to the best of our knowledge, have not yet been interfaced with electrochemical techniques.…”

Section: Lipid Membranes and Nanopore Signal Transductionmentioning

confidence: 99%

Bioinspired assemblies and plasmonic interfaces for electrochemical biosensing

Hinman

Cheng

2016

Journal of Electroanalytical Chemistry

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Electrochemical biosensing represents a collection of techniques that may be utilized for capture and detection of biomolecules in both simple and complex media. While the instrumentation and technological aspects play important roles in detection capabilities, the interfacial design aspects are of equal importance, and often, those inspired by nature produce the best results. This review highlights recent material designs, recognition schemes, and method developments as they relate to targeted electrochemical analysis for biological systems. This includes the design of electrodes functionalized with peptides, proteins, nucleic acids, and lipid membranes, along with nanoparticle mediated signal amplification mechanisms. The topic of hyphenated surface plasmon resonance assays is also discussed, as this technique may be performed concurrently with complementary and/or confirmatory measurements. Together, smart materials and experimental designs will continue to pave the way for complete biomolecular analyses of complex and technically challenging systems.

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“…17 By inclusion of functionalized PEGylated lipids, the lipodisks can be stably immobilized onto solid supports by covalent coupling. 18,19 A specific advantage of lipodisks is that both sides of the lipid bilayer are exposed to the bulk solution. It is therefore possible to incorporate an integral membrane protein into the disk and always have the active site exposed to the surrounding media.…”

Section: Introductionmentioning

confidence: 99%

“…It is therefore possible to incorporate an integral membrane protein into the disk and always have the active site exposed to the surrounding media. Furthermore, the disks are usually immobilized perpendicular to the support, 19 thus avoiding interactions between the protein and the solid surface. Also, in contrast to other planar lipid bilayer structures, such as nanodiscs 20 and bicelles, 21 lipodisks can be prepared with a wide range of compositions and size distributions, and they do not require the inclusion of any protein besides the one of interest.…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, it has been shown that lipodisks can be prepared with lipid compositions mimicking those of actual cell membranes. 22 The usefulness of immobilized lipodisks is illustrated by recent reports concerning the study of lipid-peptide interactions, 19,23 drugmembrane partitioning 22,[24][25][26] and the binding of the peripheral membrane protein COX-1. 18,27 In the present report we take the lipodisk-concept one step further and demonstrate for the first time how the interactions between an integral membrane protein and small molecules can be successfully studied using proteolipodisks covalently coupled to chromatographic media.…”

Section: Introductionmentioning

confidence: 99%

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Lipodisks integrated with weak affinity chromatography enable fragment screening of integral membrane proteins

Duong-Thi

Bergström

Edwards

et al. 2016

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Membrane proteins constitute the largest class of drug targets but they present many challenges in drug discovery. Importantly, the discovery of potential drug candidates is hampered by the limited availability of efficient methods for screening drug-protein interactions. In this work we present a novel strategy for rapid identification of molecules capable of binding to a selected membrane protein. An integral membrane protein (human aquaporin-1) was incorporated into planar lipid bilayer disks (lipodisks), which were subsequently covalently coupled to porous derivatized silica and packed into HPLC columns. The obtained affinity columns were used in a typical protocol for fragment screening by weak affinity chromatography (WAC), in which one hit was identified out of a 200 compound collection. The lipodisk-based strategy, which ensures a stable and native-like lipid environment for the protein, is expected to work also with other membrane proteins and screening procedures.

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Label-Free Characterization of Peptide–Lipid Interactions Using Immobilized Lipodisks

Abstract: The presented method constitutes a useful tool for fundamental studies of peptidemembrane interaction and can also be applied to optimize the design of lipodisks for, e.g., sustained release of antimicrobial peptides in therapeutic applications.

Cited by 12 publications

References 42 publications

Tumor-Targeted Delivery of the p53-Activating Peptide VIP116 with PEG-Stabilized Lipodisks

Tumor-Targeted Delivery of the p53-Activating Peptide VIP116 with PEG-Stabilized Lipodisks

Bioinspired assemblies and plasmonic interfaces for electrochemical biosensing

Lipodisks integrated with weak affinity chromatography enable fragment screening of integral membrane proteins

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