Label-free colorimetric assay for arsenic(III) determination based on a truncated short ssDNA and gold nanoparticles

Zhang, Dongwei; Liu, Yang; Ding, Jingtao; Hayat, Kashif; Zhan, Xuejia; Zhou, Pei; Zhang, Dan

doi:10.1007/s00604-020-04697-7

Cited by 19 publications

(3 citation statements)

References 40 publications

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“…Gold nanoparticles (AuNPs) were synthesized by citrate (23,24). A 2% chloroauric acid solution (200 mL) was boiled under stirring conditions, and 6 mL of 2% trisodium citrate solution was quickly added.…”

Section: Detection Of Hla-g Based On Aunps-aptamer Sensing Methodsmentioning

confidence: 99%

Novel nucleic acid aptamer gold (Au)-nanoparticles (AuNPs-AptHLA-G5-1 and AuNPs-AptHLA-G5-2) to detect the soluble human leukocyte antigen G5 subtype (HLA-G5) in liquid samples

Wang

Yao

2021

Ann Transl Med

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Background: The human leukocyte antigen G5 subtype (HLA-G5) is a major histocompatibility complex (MHC) molecule that is selectively expressed at the maternal-foetal tissue interface and is required for the successful implantation of the in vitro fertilized embryo. It is critical to detect HLA-G5, especially HLA-G5 expression in embryo fluid, during in vitro embryo incubation and culture. However, the specificity and sensitivity of traditional ELISA methods to detect sHLA-G5 are insufficient. This work aimed to explore novel nucleic acid aptamer gold (Au)-nanoparticles to detect soluble HLA-G5 in liquid samples.Methods: Soluble HLA-G5 was obtained using a prokaryotic expression system, and two novel aptamers (HLA-G5-Apt1 and HLA-G5-Apt2) detecting HLA-G5 were screened by the Systematic Evolution of Ligands by Exponential Enrichment (SELEX) method. Small (10 nm) gold nanoparticles (AuNPs) were incubated with AptHLAs to form two novel nucleic acid aptamers: Au-nanoparticles (AuNPs-AptHLA-G5-1 and AuNPs-AptHLA-G5-2). Results:The results showed that AptHLA-G5-1 and AptHLA-G5-2 have a high affinity for HLA-G5 and can detect its presence in liquid samples. Using the colorimetric sensing method, AuNPs-AptHLA-G1 had a detection limit as low as 20 ng/mL (recovery range between 98.7% to 102.0%), while AuNPs-AptHLA-G2 had a detection limit as low as 20 ng/mL (recovery range between 98.9% to 103.6%).Conclusions: Our work demonstrates that novel AuNPs are efficient detectors for HLA-G5 and are useful for diagnosis and treatment in the field of obstetrics-gynaecology.

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Section: Detection Of Hla-g Based On Aunps-aptamer Sensing Methodsmentioning

confidence: 99%

Novel nucleic acid aptamer gold (Au)-nanoparticles (AuNPs-AptHLA-G5-1 and AuNPs-AptHLA-G5-2) to detect the soluble human leukocyte antigen G5 subtype (HLA-G5) in liquid samples

Wang

Yao

2021

Ann Transl Med

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“…Electrochemical detection of As(III) using linker‐Free Magnetite‐decorated Gold Nanoparticles (Fe 3 O 4 ‐Au) has a range of detection of 1–100 μg/L and a detection limit of 0.22 μg/L (Sedki et al., 2021). Detection of As(III) using a label‐free colorimetric assay based on gold nanoparticles and truncated short ssDNA having a range of detection of 1–30 μg/L and 30–100 μg/L with a detection limit of 0.18 μg/L (Zhang et al., 2021). The different biosensor mechanisms for the detection of arsenic have been described in Exhibit 9.…”

Section: Detection Of Arsenic In An Aqueous System Using Biosensormentioning

confidence: 99%

A comprehensive review of arsenic contamination in India with an emphasis on its detection through biosensors and bioremediation from the aqueous system

Maity,

Bose,

Dokania

et al. 2023

Environmental Quality Mgmt

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In India, where millions of people use groundwater for drinking, arsenic pollution in groundwater is a major environmental and public health issue. The review begins by analyzing the sources and causes of groundwater arsenic pollution, including natural geological processes and anthropogenic activities like mining and industrial discharge. It emphasizes India's most arsenic‐contaminated regions and the health risks of long‐term exposure. The review's second section focuses on the use of biosensors to find arsenic in groundwater. A potential method is provided by biosensors because of their great sensitivity, selectivity, and quick response. The benefits and drawbacks of several types of biosensors, including optical, electrochemical, and microbiological biosensors, are discussed. The utilization of nanomaterials, biomolecular recognition components, and miniature devices are only a few of the most recent developments in biosensor technology for arsenic detection that are studied. The review's last section discusses methods for bio‐remediating aqueous systems that have been contaminated with arsenic. Various bioremediation techniques are covered in detail, including microbial degradation, phytoremediation, and biosorption. The promise for removing arsenic using native microbes and plant species is highlighted, along with the difficulties of widespread application and long‐term sustainability. Overall, focusing on the detection of arsenic using biosensors and investigating bioremediation techniques, this review offers a thorough grasp of the arsenic poisoning in groundwater in India. In order to combat arsenic contamination and protect the health and wellbeing of the impacted populations in India, it emphasizes the need for interdisciplinary research and collaboration among scientists, engineers, policymakers, and local communities.

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“…To date, many rapid detection technologies have also been applied to the detection of As(III) ions, including antibody antigen immunoassay technology, 19 aptamer technology 18,20 and ion imprinting recognition detection technology. 21,22 Among them, ion imprinting technology (IIT), which is based on the use of the target substance as a template to form a similar lock-key structure through the combination of a monomer and cross-linking agent, 23 has been proved as a feasible method because of its specific imprinting cavities and strong selective adsorption for target ions compared with the other two methods, 24 and it can be sufficiently used in the rapid detection, adsorption and pretreatment of heavy metal ions.…”

Section: Introductionmentioning

confidence: 99%

Preparation of efficient ion-imprinted polymers for selectively removing and detecting As(iii) from the aqueous phase

et al. 2022

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Label-free colorimetric assay for arsenic(III) determination based on a truncated short ssDNA and gold nanoparticles

Cited by 19 publications

References 40 publications

Novel nucleic acid aptamer gold (Au)-nanoparticles (AuNPs-AptHLA-G5-1 and AuNPs-AptHLA-G5-2) to detect the soluble human leukocyte antigen G5 subtype (HLA-G5) in liquid samples

Novel nucleic acid aptamer gold (Au)-nanoparticles (AuNPs-AptHLA-G5-1 and AuNPs-AptHLA-G5-2) to detect the soluble human leukocyte antigen G5 subtype (HLA-G5) in liquid samples

A comprehensive review of arsenic contamination in India with an emphasis on its detection through biosensors and bioremediation from the aqueous system

Preparation of efficient ion-imprinted polymers for selectively removing and detecting As(iii) from the aqueous phase

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