Label-free colorimetric detection of serum cysteine using Ag-NP probes in the presence of Be²⁺ ions

Abstract

Amino acids play a crucial role in a variety of significant metabolic processes and their homeostasis is essential for cell growth and survival.

Green synthesis of silver nanoparticles from corn cob aqueous extract for colorimetric cysteine detection in serum simulated with cysteine samples

“…A spectrophotometer was used to obtain the UV-vis spectra of Ag-NPs (UV-1800, Shimadzu, Japan), in the fast-speed mode with a spectral scanning rangeof 300-700 nm [13]and the scan intermissionwas 0.5 nm. A transmission electron microscopy JEM-2100 (TEM; JEOL, Japan) was used to investigate the shape and distribution of the AgNPs, which operated at a 200-kV accelerating voltage.…”

“…uorescence analysis [11], and electrochemical voltammetry are among the sensitive and selective methods being developed for the detection and measurement of cysteine in environmental, pharmaceutical, and biological samples or precursors [12].These processes depend on redox chemistry or derivative products of chromo pore/ uorescent groups, and they necessitate high temperatures, specialized instruments, and the use of powerful and harmful reagents to improve the detection of trace components and the removal of basic disruptions in a cost-effective manner. Therefore, colorimetric sensing differs from earlier approaches in that it relies on a colour shift in the nanoparticles that can be observed and analyzed with the naked eye and a basic UV-vis spectrometer [13]. Its more convenient to use, but it's also less expensive.…”

“…Because of the advancement of nanotechnology, colorimetric detection based on silver nanoparticles has recently been recognized a promising strategy for detecting cysteine [13,14]. Due to their high valueabsorption coe cient and range dependent optical features.…”

A paper-based point-of-care device for the detection of cysteine using gold nanoparticles from whole blood

“…13 To overcome these limitations, noble metal nanoparticle (NP)based colorimetric sensing is currently believed to have a promising way of developing an elegant sensing method that enables the rapid quantication of analytes together with extreme specicity and ultra-sensitivity. [14][15][16][17] In the past decade, microuidic paper-based analytical devices (mPADs) have been demonstrated for quantitative analysis in different applications, such as in the medical eld, healthcare, and environmental monitoring. [18][19][20] mPADs are lowcost, easy-to-fabricate miniaturized systems that are suitable for sample-to-answer applications.…”

“…13 To overcome these limitations, noble metal nanoparticle (NP)-based colorimetric sensing is currently believed to have a promising way of developing an elegant sensing method that enables the rapid quantification of analytes together with extreme specificity and ultra-sensitivity. 14–17…”