Label-free colorimetric estimation of proteins using nanoparticles of silver

Dash, Debabrata; Shrivastava, Siddhartha

doi:10.5101/nml.v2i3.p164-168

Cited by 3 publications

(2 citation statements)

References 21 publications

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“…Nanoparticles (NPs) have been extensively explored in diagnostic and therapeutic contexts, with potential applications to drug delivery, photothermal therapy, diagnostic imaging, photodynamic therapy, nucleic acid delivery, and implantable devices [1][2][3][4][5][6]. NPs offer some advantages:…”

Section: Introductionmentioning

confidence: 99%

Cell Membrane Coating Technology: A Promising Strategy for Biomedical Applications

et al. 2019

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Cell membrane coating technology is an approach to the biomimetic replication of cell membrane properties, and is an active area of ongoing research readily applicable to nanoscale biomedicine. Nanoparticles (NPs) coated with cell membranes offer an opportunity to unite natural cell membrane properties with those of the artificial inner core material. The coated NPs not only increase their biocompatibility but also achieve effective and extended circulation in vivo, allowing for the execution of targeted functions. Although cell membrane-coated NPs offer clear advantages, much work remains before they can be applied in clinical practice. In this review, we first provide a comprehensive overview of the theory of cell membrane coating technology, followed by a summary of the existing preparation and characterization techniques. Next, we focus on the functions and applications of various cell membrane types. In addition, we collate model drugs used in cell membrane coating technology, and review the patent applications related to this technology from the past 10 years. Finally, we survey future challenges and trends pertaining to this technology in an effort to provide a comprehensive overview of the future development of cell membrane coating technology.

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Section: Introductionmentioning

confidence: 99%

Cell Membrane Coating Technology: A Promising Strategy for Biomedical Applications

et al. 2019

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“…Gold nanoparticles (GNPs) and other NPs have emerged as one of the most robust and flexible tools in cancer research. Their applications cover the full breadth of the clinical spectrum from enhancing diagnostic imaging to photothermal and photodynamic therapy, drug delivery, and radiosensitization [1,2]. NPs were found to enter cells mostly via receptor-mediated endocytosis (RME) [3].…”

Section: Introductionmentioning

confidence: 99%

Size dependent gold nanoparticle interaction at nano-micro interface using both monolayer and multilayer (tissue-like) cell models

Yohan

Yang

et al. 2016

SPIE Proceedings

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Gold nanoparticles (GNPs) are emerging as a novel tool to improve existing cancer therapeutics. GNPs are being used as radiation dose enhancers in radiation therapy as well as anticancer drugs carriers in chemotherapy. However, the success of GNP-based therapeutics depends on their ability to penetrate tumor tissue. GNPs of 20 and 50 nm diameters were used to elucidate the effects of size on the GNP interaction with tumor cells at monolayer and multilayer level. At monolayer cell level, smaller NPs had a lower uptake compared to larger NPs at monolayer cell level. However, the order was reversed at tissue-like multilayer level. The smaller NPs penetrated better compared to larger NPs in tissue-like materials. Based on our study using tissue-like materials, we can predict that the smaller NPs are better for future therapeutics due to their greater penetration in tumor tissue once leaving the leaky blood vessels. In this study, tissue-like multilayer cellular structures (MLCs) were grown to model the post-vascular tumor environment. The MLCs exhibited a much more extensive extracellular matrix than monolayer cell cultures. The MLC model can be used to optimize the nano-micro interface at tissue level before moving into animal models. This would accelerate the use of NPs in future cancer therapeutics.

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Biosynthesis of Silver Nanoparticles Using Plant Extract and its Anti-Plasmodial Property

Panneerselvam

Murugan

Amerasan

2015

AMR

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Metallic nanoparticles have received great attention from chemists, physicists, biologists and engineers who wish to use them for the development of a new generation of nanodevices. In the present Communication, a completely “green” chemistry method for producing silver nanoparticles is introduced. The process is simple, environmentally benign, and quite efficient. Green nanoparticle synthesis has been achieved using environmentally acceptable plant extract and ecofriendly reducing and capping agents. In particular, silver nanoparticles are proved to have potential antibacterial, antifungal and antiplasmodial and antimicrobial properties. The present study was aimed to identify the antiplasmodial activity of green synthesised silver nanoparticles (AgNPs) using aqueous extract of plantEuphorbia hirtaagainstP.falciparum. Nanoparticles are being used in many commercial applications. It was found that aqueous silver ions can be reduced by aqueous extract of plant to generate extremely stable silver nanoparticles in water. The bio-reduced silver nanoparticles were appropriately characterized by UV–vis spectrum, Scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX) and X-ray diffraction (XRD). The formation of the AgNPs synthesized from the XRD spectrum compared with the standard confirmed spectrum of silver particles formed in the present experiments were in the form of nanocrystals, as evidenced by the peaks at 2θ values of =28.01°, 32.41°, 46.44°, 55.05° and 57.75°. The scanning electron micrograph (SEM) showed structures of spherical, cubic shape, and the size range was found to be 30–60 nm. The EDX spectra showed the purity of the material and the complete chemical composition of the synthesized AgNPs. The parasitic inhibition was dose-dependent. The synthesized AgNPs showed considerable antiplasmodial activity than the crude methanol and aqueous leaf extract ofE.hirta. The maximum efficacy was

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Label-free colorimetric estimation of proteins using nanoparticles of silver

Cited by 3 publications

References 21 publications

Cell Membrane Coating Technology: A Promising Strategy for Biomedical Applications

Cell Membrane Coating Technology: A Promising Strategy for Biomedical Applications

Size dependent gold nanoparticle interaction at nano-micro interface using both monolayer and multilayer (tissue-like) cell models

Biosynthesis of Silver Nanoparticles Using Plant Extract and its Anti-Plasmodial Property

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