Label-free cytosensing of cancer cells based on the interaction between protein and an electron-transfer carbohydrate-mimetic peptide

Sugawara, Kazuharu; Kuramitz, Hideki; Kadoya, Toshihiko

doi:10.1016/j.aca.2018.08.025

Cited by 14 publications

(9 citation statements)

References 45 publications

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“…Interestingly, an electronic transfer peptide YYYYC possessing excellent electroactivity and functionality of hydrate-mimicking peptides has also been designed. In studies, YYYYC was conjugated to myelopeptide-4, which is derived from bone marrow and fixed on electrode through collagen and measured through electrochemical impedance spectroscopy [ 193 ]. Additionally, the electrochemical nanosensors were utilized to detect the cancer stem cell which predominantly expresses the cell surface markers CD44, CD90, CD133/2, and OV-6 through their antibodies of gold electrodes [ 194 ].…”

Section: Stimuli Responsive Intelligent Nanomaterials In Cancer Thera...mentioning

confidence: 99%

Smart nanomaterials for cancer diagnosis and treatment

et al. 2022

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Innovations in nanomedicine has guided the improved outcomes for cancer diagnosis and therapy. However, frequent use of nanomaterials remains challenging due to specific limitations like non-targeted distribution causing low signal-to-noise ratio for diagnostics, complex fabrication, reduced-biocompatibility, decreased photostability, and systemic toxicity of nanomaterials within the body. Thus, better nanomaterial-systems with controlled physicochemical and biological properties, form the need of the hour. In this context, smart nanomaterials serve as promising solution, as they can be activated under specific exogenous or endogenous stimuli such as pH, temperature, enzymes, or a particular biological molecule. The properties of smart nanomaterials make them ideal candidates for various applications like biosensors, controlled drug release, and treatment of various diseases. Recently, smart nanomaterial-based cancer theranostic approaches have been developed, and they are displaying better selectivity and sensitivity with reduced side-effects in comparison to conventional methods. In cancer therapy, the smart nanomaterials-system only activates in response to tumor microenvironment (TME) and remains in deactivated state in normal cells, which further reduces the side-effects and systemic toxicities. Thus, the present review aims to describe the stimulus-based classification of smart nanomaterials, tumor microenvironment-responsive behaviour, and their up-to-date applications in cancer theranostics. Besides, present review addresses the development of various smart nanomaterials and their advantages for diagnosing and treating cancer. Here, we also discuss about the drug targeting and sustained drug release from nanocarriers, and different types of nanomaterials which have been engineered for this intent. Additionally, the present challenges and prospects of nanomaterials in effective cancer diagnosis and therapeutics have been discussed.

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Section: Stimuli Responsive Intelligent Nanomaterials In Cancer Thera...mentioning

confidence: 99%

Smart nanomaterials for cancer diagnosis and treatment

et al. 2022

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“…According to different prognostic indicators, leukemia patients can be divided into different prognostic levels for different treatment intensities; therefore, it is important to complete the comprehensive examinations required for various prognostic stratification processes and to develop a personalized treatment plan. Sugawara et al found and designed an electronic transfer peptide, YYYYC [ 57 ], which had excellent electroactivity and the functionality of two hydrate-mimicking peptides [ 58 ]. They then performed an electrochemical analysis of the changes in current based on the competition between YYYYC and cancer cells for SBA.…”

Section: Electrochemical Detection Of Markers On Tumor Cellsmentioning

confidence: 99%

Electrochemical Sensors for Detection of Markers on Tumor Cells

Zhou

Zhang

2021

IJMS

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In recent years, the increasing incidence and mortality of cancer have inspired the development of accurate and rapid early diagnosis methods in order to successfully cure cancer; however, conventional methods used for detecting tumor cells, including histopathological and immunological methods, often involve complex operation processes, high analytical costs, and high false positive rates, in addition to requiring experienced personnel. With the rapid emergence of sensing techniques, electrochemical cytosensors have attracted wide attention in the field of tumor cell detection because of their advantages, such as their high sensitivity, simple equipment, and low cost. These cytosensors are not only able to differentiate tumor cells from normal cells, but can also allow targeted protein detection of tumor cells. In this review, the research achievements of various electrochemical cytosensors for tumor cell detection reported in the past five years are reviewed, including the structures, detection ranges, and detection limits of the cytosensors. Certain trends and prospects related to the electrochemical cytosensors are also discussed.

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“…A glassy carbon electrode modified with Ac-Y 4 CA 6 /collagen film the sensing of SBA using magnetic beads with Y 4 C immobilized via N-(8-maleimidocapryloxy) succinimide [26]. The electrode responses were linear and ranged between 2.5 × 10 À 12 and 1.0 × 10 À 10 M. Furthermore, the SBA sensing system was constructed using ASF-immobilized beads and electron-transfer peptide [9]. The sensitivity of SBA using this method was superior to that of previous methods because SBA was accumulated on the electrode based on the binding with Y 4 C. We measured the electrode responses for 14 days after the electrodes were fabricated (Figure S7).…”

Section: Electrochemical Approachmentioning

confidence: 99%

“…The expression and function of cellular membrane proteins based on SBA also induces apoptosis [7]. In contrast, SBA is used to monitor carbohydrates such as N-acetyl-D-galactosamine and galactosamine residues on the cell surface [8,9]. On the other hand, SBA can play the role of a food allergen that can cause atopic dermatitis, breathing disorders, gastrointestinal dysfunction, and anaphylactic shock.…”

Section: Introductionmentioning

confidence: 99%

Voltammetric Sensing of Soybean Agglutinin Using an Electrode Modified with Electron‐transfer, Carbohydrate‐mimetic/Cross‐linker‐peptide‐collagen Film

2021

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We constructed an electrochemical sensor based on an electrode modified with electron-transfer, carbohydrate-mimetic peptides on collagen film. The peptide consisted of Ac-Y 4 C combined with soybean agglutinin (SBA). To evaluate the binding between SBA and the peptide, we prepared Ac-Y 4 CA n (3-6) containing oligoalanine as a cross-linker. When SBA and Y 4 C on the electrode were incubated, the peak was decreased by the SBA uptake of the peptide. The change in the peak current using Ac-Y 4 CA 6 was the greatest of the four peptides. The calibration curve was linear and ranged from 4.0 × 10 À 14 to 1.2 × 10 À 12 M with a detection limit of 1.3 × 10 À 14 M.

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Label-free cytosensing of cancer cells based on the interaction between protein and an electron-transfer carbohydrate-mimetic peptide

Cited by 14 publications

References 45 publications

Smart nanomaterials for cancer diagnosis and treatment

Smart nanomaterials for cancer diagnosis and treatment

Electrochemical Sensors for Detection of Markers on Tumor Cells

Voltammetric Sensing of Soybean Agglutinin Using an Electrode Modified with Electron‐transfer, Carbohydrate‐mimetic/Cross‐linker‐peptide‐collagen Film

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