Label-free Detection of Antigen Protein Using a Metal Mesh Device Surface-modified by an Antibody

Abstract

A biosensor for protein detection was developed using antibody-immobilized metal mesh devices. Antihemoglobin antibodies were covalently immobilized on a metal mesh device. Extraordinary transmission with a dipped structure was observed for a metal mesh device immobilized with antihemoglobin antibodies as well as for the original metal mesh device. Hemoglobin in the mixture solution containing albumin at a hundred-fold concentration was detectable using antihemoglobin-immobilized MMDs. The detectability using the antihemoglobin-immobilized metal mesh device was similar to that of a commercially-available kit for the qualitative determination of hemoglobin.

Quantitative Measurement of Protein Using Metal Mesh Device

“…The dip in the spectra were specific phenomena in MMD, which have been reported by our group [13][14][15] and other groups. 8,9 The dip was the result of fano-like interference effect between the terahertz light transmitted directly and obliquely through MMD.…”

“…In the experiments, protein adsorption caused the dip frequency shift. [13][14][15] The magnetic permeability of the organic substances is usually less than 1. The dip frequency shift from the inductance change can be ignored due to the small magnetic permeability.…”

“…In 2011, we reported the sensing principles of MMD in the sub-millimeter range, 12 and the possibility of protein sensing adsorbed onto the MMD by the transmittance property in the terahertz (infrared) region. [13][14][15] The MMD was manufactured with the electroforming method, which enables the fabrication of the devices of various structures, sizes, and transmittance properties of arbitrary frequency (wavelength). Therefore, it is possible to utilize the specific MMD corresponding to the target substances.…”

Using Laser Interference Lithography in the Fabrication of a Simplified Micro- and Nanofluidic Device for Label-free Detection

“…[1][2][3][4][5] Recently, we developed a label-free detection method for biomolecules using our fabricated micro-and nanofluidic device. 6 This detection method is based on optical diffraction that occurs when the laser beam passes through nanostructures inside the micro-and nanofluidic device.…”

Use of metal mesh sensors with periodic microstructures to sense and separate aerosol particles in Kenya

“…When the complex refractive index is modified by adherence of a particulate substance to the metal surface, localized electric fields are influenced, and transmission patterns are altered in a frequency-sensitive manner ( Figure 2). Based on this phenomenon, MM membranes can be employed as label-free optical sensors [2][3][4][5]. Accordingly, MM sensors have attracted considerable attention because of their prospective use as simple, inexpensive biosensors.…”