Label‐Free Detection of DNA Hybridization at the Nanoscale: A Highly Sensitive and Selective Approach Using Atomic‐Force Microscopy

Zhou, Dejian; Sinniah, Kumar; Abell, Chris; Rayment, Trevor

doi:10.1002/ange.200352178

Cited by 14 publications

(14 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Loosely packed DNA monolayers assembled in the absence of Mg 2+ are necessary for improved accessibility of proteins, but such monolayers have a distinct “island” morphology since the DNA has room to lie flat on the gold surface in the absence of an applied potential (23). However, monolayers backfilled with a short chain alkanethiol such as mercaptohexanol were demonstrated to adopt an upright orientation relative to the gold surface even at open circuit (35, 36). Furthermore, these studies showed that even loosely packed films formed from longer 24 mer and 30 mer duplexes adopt an upright orientation.…”

Section: Surface Characterizationmentioning

confidence: 99%

DNA-Mediated Electrochemistry

2008

View full text Add to dashboard Cite

The base pair stack of DNA has been demonstrated as a medium for long range charge transport chemistry both in solution and at DNA-modified surfaces. This chemistry is exquisitely sensitive to structural perturbations in the base pair stack as occur with lesions, single base mismatches, and protein binding. We have exploited this sensitivity for the development of reliable electrochemical assays based on DNA charge transport at self-assembled DNA monolayers. Here we discuss the characteristic features, applications, and advantages of DNA-mediated electrochemistry.

show abstract

Section: Surface Characterizationmentioning

confidence: 99%

DNA-Mediated Electrochemistry

2008

View full text Add to dashboard Cite

show abstract

“…Increased height of hybridized molecules also result in spot sizes larger than the size of DNA molecules due to tip convolution. The changes in the height of hybridized molecules have also been used to detect hybridization25,26; however these demonstrations required nano- to micromolar level target concentrations. In addition, height measurements provide limited discrimination against the roughness of the substrate surface; therefore atomically flat substrates had to be used.…”

mentioning

confidence: 99%

DNA nanomechanics allows direct digital detection of complementary DNA and microRNA targets

Husale

Persson

Şahin

2009

Nature

142

127

View full text Add to dashboard Cite

Techniques to detect and quantify DNA and RNA molecules in biological samples have played a central role in genomics research1–3. Over the past decade, several techniques have been developed to improve detection performance and reduce the cost of genetic analysis4–10. In particular, dramatic advances in label-free methods have been reported11–17. Yet, detection of DNA molecules at concentrations below femtomolar level requires amplified detection schemes1,8. Here we report a unique nanomechanical response of hybridized DNA and RNA molecules that serves as an intrinsic molecular label. Nanomechanical measurements on a microarray surface exhibit excellent background signal rejection that allows direct detection and counting of hybridized molecules. The digital response of the sensor provides a large dynamic range that is critical for gene expression profiling. We have measured differential expressions of miRNAs in tumor samples, which has been shown to help discriminate tissue origins of metastatic tumors18. 200 picograms of total RNA is found to be sufficient for this analysis. In addition, the limit of detection in pure samples is found to be 1 attomolar. These results suggest that nanomechanical readout of microarrays promises attomolar level sensitivity and large dynamic range for the analysis of gene expression, while eliminating biochemical manipulations, amplification, and labeling.

show abstract

“…Nanopatterns of thiolated ssDNA were created on a gold surface by using nanografting, thus leading to sequence-specific DNA hybridization [92]. In the nanoshaving technique, the AFM tip mechanically removes a resister for creating nanopatterns on surfaces and then biomolecules can be immobilized onto the nanoshaved patterned surfaces using a sequential self-assembly [85, 107]. …”

Section: Nanoscale Patterning Of Biomolecules Using Molecular Recognimentioning

confidence: 99%

“…The antibody-functionalized nanotubes were site-specifically assembled on the complementary antigen regions of the trenches via molecular recognition [109]. Furthermore, multiple DNA nanopatterns of two different sequences were produced on a single gold surface by nanoshaving technique and applied to selective label-free DNA detection [107]. …”

Section: Nanoscale Patterning Of Biomolecules Using Molecular Recognimentioning

confidence: 99%

Molecular Recognition and Specific Interactions for Biosensing Applications

Kim

Kang

2008

Sensors

View full text Add to dashboard Cite

Molecular recognition and specific interactions are reliable and versatile routes for site-specific and well-oriented immobilization of functional biomolecules on surfaces. The control of surface properties via the molecular recognition and specific interactions at the nanoscale is a key element for the nanofabrication of biosensors with high sensitivity and specificity. This review intends to provide a comprehensive understanding of the molecular recognition- and specific interaction-mediated biosensor fabrication routes that leads to biosensors with well-ordered and controlled structures on both nanopatterned surfaces and nanomaterials. Herein self-assembly of the biomolecules via the molecular recognition and specific interactions on nanoscaled surfaces as well as nanofabrication techniques of the biomolecules for biosensor architecture are discussed. We also describe the detection of molecular recognition- and specific interaction-mediated molecular binding as well as advantages of nanoscale detection.

show abstract

Label‐Free Detection of DNA Hybridization at the Nanoscale: A Highly Sensitive and Selective Approach Using Atomic‐Force Microscopy

Cited by 14 publications

References 30 publications

DNA-Mediated Electrochemistry

DNA-Mediated Electrochemistry

DNA nanomechanics allows direct digital detection of complementary DNA and microRNA targets

Molecular Recognition and Specific Interactions for Biosensing Applications

Contact Info

Product

Resources

About