Label-Free Detection of Gliadin in Food by Quartz Crystal Microbalance-Based Immunosensor

Funari, Riccardo; Terracciano, Irma; Ventura, Bartolomeo Della; Ricci, Sara; Cardi, Teodoro; D’Agostino, Nunzio; Velotta, R.

doi:10.1021/acs.jafc.6b04830

Cited by 27 publications

(9 citation statements)

References 37 publications

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“…The complement C4 was determined in a range 0.08–1.6 µg/mL and relative standard deviation for the assay was around five percent. Good example of a QCM immunosensor was described by Funari and coworkers [ 20 ] who immobilized spatially oriented antibodies against gluten on gold electrodes and reached limit of detection 4 ppm for the gluten and sensitivity range was between 7.5 and 15 ppm.…”

Section: Piezoelectric Immunosensorsmentioning

confidence: 99%

Overview of Piezoelectric Biosensors, Immunosensors and DNA Sensors and Their Applications

2018

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Piezoelectric biosensors are a group of analytical devices working on a principle of affinity interaction recording. A piezoelectric platform or piezoelectric crystal is a sensor part working on the principle of oscillations change due to a mass bound on the piezoelectric crystal surface. In this review, biosensors having their surface modified with an antibody or antigen, with a molecularly imprinted polymer, with genetic information like single stranded DNA, and biosensors with bound receptors of organic of biochemical origin, are presented and discussed. The mentioned recognition parts are frequently combined with use of nanoparticles and applications in this way are also introduced. An overview of the current literature is given and the methods presented are commented upon.

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Section: Piezoelectric Immunosensorsmentioning

confidence: 99%

Overview of Piezoelectric Biosensors, Immunosensors and DNA Sensors and Their Applications

2018

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“…Recently, a sensitive immunochromatographic test for the detection of gluten (RIDA ® QUICK Gliadin) was successfully tested for detection of gliadin in foods, on surfaces, and in cleaning in place waters [47]. Several types of immunosensors have been developed by Funari et al [48], Manfredi et al [49], and Ng et al [50]. Proteomic methods offer alternative approaches for quantification of toxic prolamin proteins.…”

Section: Contamination Of Oat By Cereal Prolaminsmentioning

confidence: 99%

The Pros and Cons of Using Oat in a Gluten-Free Diet for Celiac Patients

et al. 2019

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A therapeutic gluten-free diet often has nutritional limitations. Nutritional qualities such as high protein content, the presence of biologically active and beneficial substances (fiber, beta-glucans, polyunsaturated fatty acids, essential amino acids, antioxidants, vitamins, and minerals), and tolerance by the majority of celiac patients make oat popular for use in gluten-free diet. The health risk of long-time consumption of oat by celiac patients is a matter of debate. The introduction of oat into the diet is only recommended for celiac patients in remission. Furthermore, not every variety of oat is also appropriate for a gluten-free diet. The risk of sensitization and an adverse immunologically mediated reaction is a real threat in some celiac patients. Several unsolved issues still exist which include the following: (1) determination of the susceptibility markers for the subgroup of celiac patients who are at risk because they do not tolerate dietary oat, (2) identification of suitable varieties of oat and estimating the safe dose of oat for the diet, and (3) optimization of methods for detecting the gliadin contamination in raw oat used in a gluten-free diet.

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“…Among the latest experimental immunosensors, it is worth mentioning the immunosensor developed by Funari and colleagues [39]. By combining a cheap and robust piezoelectric transducer (quartz crystal microbalance technology) and the photonic immobilization technique, they managed to obtain a LOD of about 4 ppm and a sensitivity of about 7.5-15 ppm.…”

Section: Immunosensors Dipsticks and Lateral Flow Devices Western Blotmentioning

confidence: 99%

Immunological Methods in Gluten Risk Analysis: A Snapshot

Melini¹,

Melini²

2018

Safety

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Gluten is among the 14 major food allergens officially recognized by Regulation (EU) No. 1169/2011. The risk to coeliac patients from gluten presence in the food products they consume is likely due to the unintentional contamination of naturally gluten-free (GF) and GF-labelled products, or to hidden sources of gluten in processed GF products. The aim of this paper is to provide a snapshot of gluten risk analysis, with emphasis on immunological methods currently used in gluten detection. The study highlights that immunoassays have some advantages over other analytical methods in gluten determination and are suitable for routine tests. However, some factors (e.g., complexity of the food matrix, type of the applied antibody, gluten extraction procedures and lack of reference material) affect the reliability of obtained results. Hence, efforts are required at an analytical level to overcome the drawbacks of the immunological methods currently available. Harmonization is necessary, so as to assist both consumers in making safe food choices, and the food industry in gluten risk assessment, management and communication.

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Label-Free Detection of Gliadin in Food by Quartz Crystal Microbalance-Based Immunosensor

Cited by 27 publications

References 37 publications

Overview of Piezoelectric Biosensors, Immunosensors and DNA Sensors and Their Applications

Overview of Piezoelectric Biosensors, Immunosensors and DNA Sensors and Their Applications

The Pros and Cons of Using Oat in a Gluten-Free Diet for Celiac Patients

Immunological Methods in Gluten Risk Analysis: A Snapshot

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