Label-free detection of neuron–drug interactions using acoustic and Kelvin vibrational fields

Cheran, Larisa-Emilia; Cheung, Shilin; Chawaf, Arij Al; Ellis, Jonathan S.; Belsham, Denise D.; MacKay, William A.; Lovejoy, David A.; Thompson, Michael

doi:10.1039/b615476j

Cited by 18 publications

(19 citation statements)

References 67 publications

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“…Abdel-Salam et al reported that Cerebrolysin prevented neural loss by inhibiting ROS development and LPO (22). Cheran et al used an experimental method to show the protective effect of Cerebrolysin on acoustic nerve cells (23). Our findings and literature data show that Cerebrolysin effectively inhibits acoustic nerve damage caused by cisplatin-induced oxidative stress.…”

Section: C 1dsupporting

confidence: 69%

The Effect of Cerebrolysin on Cisplatin Induced Oxidative Acoustic Nerve Injury in Rats

Erhan¹,

Bayram²,

Kurt³

et al. 2020

Acta Poloniae Pharmaceutica - Drug Research

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One of the major side effects of dose-limiting cisplatin is ototoxicity, resulting in restricted clinical use. Cerebrolysin is a neuroprotective, neurotrophic and antioxidant drug derived from the brains of pigs. No studies have investigated the positive effects of Cerebrolysin on the damage to auditory nerves caused by cisplatin-induced oxidative stress. Eighteen rats were randomly divided into three groups of six: the Cerebrolysin and cisplatin group (CCG), the cisplatin group (CG) and the healthy group (HG). The CCG was administered 2.5 mL/kg of Cerebrolysin via subcutaneous injection daily, while the CG and HG were administered physiological saline solutions daily. Every other day, after receiving their dose of Cerebrolysin or physiological saline solution, the CCG and CG groups were administered 5 mg/kg of cisplatin via intraperitoneal injection (IP) every other day. The substances were administered for eight days. In acoustic nerve tissue, while the CCís oxidant parameters were significantly higher (p < 0.0001) than those of the CCG and HG groups, its antioxidant parameters were also significantly lower (p < 0.0001). Cerebrolysin antagonized the balance of oxidants and antioxidants in acoustic nerve tissue, resulting in higher concentrations of oxidants. The results suggested that Cerebrolysin may be useful in the treatment of cisplatin-induced oxidative acoustic nerve injury.

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Section: C 1dsupporting

confidence: 69%

The Effect of Cerebrolysin on Cisplatin Induced Oxidative Acoustic Nerve Injury in Rats

Erhan¹,

Bayram²,

Kurt³

et al. 2020

Acta Poloniae Pharmaceutica - Drug Research

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“…Fig. 60 When forskolin was added at the critical concentration of 10 À4 M, a significant change of 300 mV in electrical contact potential was detected by the SKN. The probe vibrates with a high frequency at a distance d 0 from the neurons and with small amplitude, d 1 .…”

Section: The Kelvin Fieldmentioning

confidence: 90%

“…9 illustrates the application of the SKN to surface-attached neurons. 60 For the same neuron line, a dramatic 850% variation in cAMP levels was detected. The neurons, which are grown on a gold surface, alter the work function of the metal by their presence via dielectric properties.…”

Section: The Kelvin Fieldmentioning

confidence: 93%

“…Accordingly, instigated changes in the neuron result in an alteration of coupling at the membrane-sensor interface, and of signals associated with cytoplasm properties. 60 These plots of acoustic parameters illustrate the oscillation in signals that are then followed by a damping effect instigated by the drug (betaferon is known to have strong effects on neural tissue). Fig.…”

Section: The Vibrational Field and Acoustic Wave Detectionmentioning

confidence: 98%

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Coupling of neurons with biosensor devices for detection of the properties of neuronal populations

2008

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The in vitro detection of the neural biophysical chemistry of populations of neurons is an important emerging area of research. This critical review describes the current methodologies, challenges and future prospects for this exciting field of research. There are different classes of techniques for the study of neuron-based systems. These include devices to measure inter-neuron contact and connectivity, microelectrodes for the determination of extracellular metabolic products, and sensors employed for the evaluation of complex neuron-small molecule interactions, toxicity, and mutagenicity of anti-tumor drugs. Since the neuron is an electrogenic cell and a complex biological entity capable of effecting recognition, the main emphasis of this article will be placed on devices based on nerve-cell networks that are able to electrically detect neuron-active compounds and specific pharmacological activity. Such neuron-based devices can be used to measure numerous neurological events with a high degree of sensitivity. Examples include the influence of different neuro-active compounds on neuronal function, the effects of neurotransmitters and neuro-modulators, changes in membrane potential, transmission effects that influence the propagation of the action potential, and the manner through which neuro-chemicals can influence ion channels. Moreover, these devices posses promising potential for the testing and development of novel neuron-active drugs and fundamental neurological research to further the understanding of brain activity. The inner workings of the human mind remain largely unknown and the key to comprehending it may rely on how molecules can initiate and influence synchronous neural oscillations, and the phenomenon of resonance in neural cells. The knowledge acquired in such detailed investigations can lead to the future development of regenerative medicines, neurochips and biocomputers, intelligent prosthetic devices and new applications that integrate neurobiology with molecular electronics (69 references).

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“…In terms of the future, such experiments could lead to a correlation of tissue biophysical processes with, for example, treatment with drugs. This approach was employed by our group in an examination of the response of hypothalamic neurons to neurotrophic factors [17]. Using careful control of temperature and acoustic cell flow conditions, the tissue samples were exposed to various intensities of white light.…”

Section: Introductionmentioning

confidence: 99%

Radiation-Activated Pre-Differentiated Retinal Tissue Monitored by Acoustic Wave Biosensor

Cheran

Thompson

2020

Sensors

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A thickness-shear mode acoustic wave biosensor operated within a flow-through system was used to examine the response of mouse retinal tissue to radiation. Control experiments conducted with respect to exposure of the bare gold electrodes of the device under various conditions of light intensity and bathing solution yielded reversible changes in resonant frequency (Fs) and motional resistance (Rm). The magnitude of transient changes was proportional to light intensity, but independent of solution type. These alterations in acoustic parameters were ascribed to acoustic coupling phenomena at the electrode-to-liquid interface. Pre-differentiated retina from mouse samples deposited on the thickness shear mode (TSM) electrode exposed to a high light intensity condition also exhibited reversible changes in both Fs and Rm, compared to control experiments involving a coating used to attach the tissue to the electrode. In this case, the radiation-instigated reversible responses for both acoustic parameters exhibited a reduction in magnitude. The changes are ascribed to the alteration in viscoelasticity of the retinal matrix on the TSM electrode surface. The precise biophysical mechanism responsible for the changes in Fs and Rm remains a challenge, given the complex make up of retinal tissue.

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Label-free detection of neuron–drug interactions using acoustic and Kelvin vibrational fields

Cited by 18 publications

References 67 publications

The Effect of Cerebrolysin on Cisplatin Induced Oxidative Acoustic Nerve Injury in Rats

The Effect of Cerebrolysin on Cisplatin Induced Oxidative Acoustic Nerve Injury in Rats

Coupling of neurons with biosensor devices for detection of the properties of neuronal populations

Radiation-Activated Pre-Differentiated Retinal Tissue Monitored by Acoustic Wave Biosensor

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