Label-free detection of ovarian cancer biomarkers using whispering gallery mode imaging

Huckabay, Heath A.; Wildgen, Sarah M.; Dunn, Robert C.

doi:10.1016/j.bios.2013.01.072

Cited by 37 publications

(41 citation statements)

References 53 publications

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“…A simulation of fluid flows needed in a microfluidic chip to obtain optimal performance with a similar integrated photonic device for IL-6 has been described [249]. Dunn and colleagues have measured different cytokines using whispering-gallery mode in microfluidic devices [250, 251]. …”

Section: Optical Methodsmentioning

confidence: 99%

Bioanalytical chemistry of cytokines – A review

Stenken

Poschenrieder

2015

Analytica Chimica Acta

243

172

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Cytokines are bioactive proteins produced by many different cells of the immune system. Due to their role in different inflammatory disease states and maintaining homeostasis, there is enormous clinical interest in the quantitation of cytokines. The typical standard methods for quantitation of cytokines are immunoassay-based techniques including enzyme-linked immusorbent assays (ELISA) and bead-based immunoassays read by either standard or modified flow cytometers. A review of recent developments in analytical methods for measurements of cytokine proteins is provided. This review briefly covers cytokine biology and the analysis challenges associated with measurement of these biomarker proteins for understanding both health and disease. New techniques applied to immunoassay-based assays are presented along with the uses of aptamers, electrochemistry, mass spectrometry, optical resonator-based methods. Methods used for elucidating the release of cytokines from single cells as well as in vivo collection methods are described.

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Section: Optical Methodsmentioning

confidence: 99%

Bioanalytical chemistry of cytokines – A review

Stenken

Poschenrieder

2015

Analytica Chimica Acta

243

172

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“…Fiber tips have also shown high efficiency for exciting WGMs in fluorescent microbeads [315]. Fluorescent droplets in a microfluidic structure were used to read out WGMs [316], while fluorophores in the solution surrounding spherical beads have been shown to aid the fluorescence readout of WGMs [317]. Fluorescent beads as WGM sensors have also shown interesting in vivo applications, for transducing forces that deform a polymer WGM sensor during phagocytosis of such beads by cells [248].…”

Section: Sensingmentioning

confidence: 99%

“…Recent demonstrations involve the multiplexed detection of cancer biomarkers [290,292,330] and secreted proteins [283]. Multiplexed detection is also important for WGM sensing modalities that rely on WGM imaging [317]. …”

Section: Sensingmentioning

confidence: 99%

Whispering gallery mode sensors

2015

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We present a comprehensive overview of sensor technology exploiting optical whispering gallery mode (WGM) resonances. After a short introduction we begin by detailing the fundamental principles and theory of WGMs in optical microcavities and the transduction mechanisms frequently employed for sensing purposes. Key recent theoretical contributions to the modeling and analysis of WGM systems are highlighted. Subsequently we review the state of the art of WGM sensors by outlining efforts made to date to improve current detection limits. Proposals in this vein are numerous and range, for example, from plasmonic enhancements and active cavities to hybrid optomechanical sensors, which are already working in the shot noise limited regime. In parallel to furthering WGM sensitivity, efforts to improve the time resolution are beginning to emerge. We therefore summarize the techniques being pursued in this vein. Ultimately WGM sensors aim for real-world applications, such as measurements of force and temperature, or alternatively gas and biosensing. Each such application is thus reviewed in turn, and important achievements are discussed. Finally, we adopt a more forward-looking perspective and discuss the outlook of WGM sensors within both a physical and biological context and consider how they may yet push the detection envelope further.

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“…In such applications, the potential for real-time, multiplexed detection of proteins following a separation step offers exciting opportunities. 81,88,97 …”

Section: Future Prospects: Label-free Biosensing For Multiplexed Detementioning

confidence: 99%

Recent advances in microscale western blotting

2016

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Western blotting is a ubiquitous tool used extensively in the clinical and research settings to identify proteins and characterize their levels. It has rapidly become a mainstay in research laboratories due to its specificity, low cost, and ease of use. The specificity arises from the orthogonal processes used to identify proteins. Samples are first separated based on size and then probed with antibodies specific for the protein of interest. This confirmatory approach helps avoid pitfalls associated with antibody cross-reactivity and specificity issues. While the technique has evolved since its inception, the last decade has witnessed a paradigm shift in Western blotting technology. The introduction of capillary and microfluidic platforms has significantly decreased time and sample requirements while enabling high-throughput capabilities. These advances have enabled Western analysis down to the single cell level in highly parallel formats, opening vast new opportunities for studying cellular heterogeneity. Recent innovations in microscale Western blotting are surveyed, and the potential for enhancing detection using advances in label-free biosensing is briefly discussed.

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Label-free detection of ovarian cancer biomarkers using whispering gallery mode imaging

Cited by 37 publications

References 53 publications

Bioanalytical chemistry of cytokines – A review

Bioanalytical chemistry of cytokines – A review

Whispering gallery mode sensors

Recent advances in microscale western blotting

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