Label-free detection of Phytophthora ramorum using surface-enhanced Raman spectroscopy

Yüksel, Sezin; Schwenkbier, Lydia; Pollok, Sibyll; Weber, Karina; Cialla‐May, Dana; Popp, Jürgen

doi:10.1039/c5an01156f

Cited by 40 publications

(15 citation statements)

References 52 publications

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“…The distinctive peaks at 734 cm À1 and 1330 cm À1 are attributed to adenine, whereas strong bands at 1338 cm À1 and 1461 cm À1 are characteristic of guanine. [67][68][69] The intense bands at 802 cm À1 and 1279 cm À1 are unique to uracil in these three sequences. Given the high molecular weight, relatively strong Raman signals were observed even with a small drop of RNA solution (50 mL) on the plasmonic paper.…”

Section: Resultsmentioning

confidence: 86%

Sandwiching analytes with structurally diverse plasmonic nanoparticles on paper substrates for surface enhanced Raman spectroscopy

et al. 2019

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Section: Resultsmentioning

confidence: 86%

Sandwiching analytes with structurally diverse plasmonic nanoparticles on paper substrates for surface enhanced Raman spectroscopy

et al. 2019

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“…Enzymatically generated nanoparticles (EGNPs), formed out of flat plates of silver crystals, show a desert‐rose‐ or flower‐like structure and are noted as powerful bottom‐up SERS substrates . These structures have great capability in bioanalytics, e.g., the label‐free detection of plant pathogens or the detection of Sudan III in food matrixes, as shown recently. Because the EGNPs are distributed statistically (see Figure A,B), which leads to spatial variations of the SERS signal, developing a method that provides a regular arrangement of flower‐like nanostructures across the surface was desired to improve their characteristics for SERS‐based detection schemes.…”

Section: Resultsmentioning

confidence: 99%

TopUp Plasmonic Arrays for Surface‐Enhanced Raman Spectroscopy

Patze

Huebner

Weber

et al. 2016

Adv Materials Inter

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III in paprika powder, [ 11 ] azorubine in drinks, [ 12 ] or melamine in milk. [ 13 ] In clinical application schemes, SERS shows high potential as a point-of-care method for the online monitoring of morphine, methadone, or dopamine [ 14 ] in intravenous delivery tubing or of levofl oxacin in urine. [ 15 ] Moreover, the detection of environmentally relevant and harmful substances, i.e., arsenic (III) in lake water samples [ 16 ] or veterinary drugs such as metronidazole and ronidazole in various water sources, [ 17 ] extends the application fi eld of SERS. All of the above-mentioned application examples share the enhancement of the naturally weak Raman signal by employing nanostructured metal surfaces, so-called SERS substrates.The applied nanostructured SERS substrates are commonly generated via wet chemistry [ 18 ] and bottom-up [ 19 ] or topdown [ 20,21 ] approaches. Metal colloids can be prepared in large quantities via wet chemical reactions, e.g., the reduction of silver or gold salts to the pure metal. In general, metallic nanoparticle suspensions are characterized by easy handling and fast production in high quantities. In contrast, limitations are related to their low time stability, poor batch-to-batch reproducibility, and spectral interference originating from the reaction participants. As an alternative, laser ablation techniques are employed to fabricate metal nanoparticles without stabilization agents on their surfaces. [ 22 ] Moreover, planar substrates produced by immobilization of metallic nanoparticles based on a bottom-up technique are characterized by low homogeneity across a large area and low batch-to-batch quality.Top-down techniques can overcome some of the above-mentioned limitations by structuring thin metal layers using lithographic techniques, e.g., electron beam lithography (EBL) [ 21,23 ] or nanoimprint lithography (NIL). [ 24 ] These procedures lead to highly homogenous planar substrates with well-defi ned structures and plasmonic resonance tunabilities. However, due to the multiple processing steps required for top-down techniques, the manufacturing time for a given structure is extensive and the process is expensive. Thus, the top-down production of SERS active substrates is realized in small quantities.By an appropriate combination of bottom-up and top-down techniques, the creation of a SERS active substrate that uses the advantages and avoids the drawbacks of each applied fabrication technique becomes feasible. In principle, a sophisticated top-down approach is improved by a fast and easy bottom-up method. As an example, particle-in-cavity structures [ 25 ] were created for which the template (the cavity structure) is fabricated by nanosphere lithography (NSL) [ 25 ] following a porous By combining a bottom-up process with top-down fabricated templates, the benefi ts of both methods are joined by creating so-called TopUp plasmonic arrays for surface-enhanced Raman spectroscopy (SERS). The morphological and optical characterization of the TopUp substrates illustrates exceptional...

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“…Most recently, surfaceenhanced Raman scattering (SERS) has been used for labelfree and species-specific detection of P. ramorum in infected rhododendron (Rhododendron spp.) leaves (Yuksel et al 2015). Accurate detection of the laurel wilt pathogen (R. lauricola) has been difficult, due in part to the occurrence of related fungi in the same affected plant.…”

Section: Advances In Molecular Tools For Detectionmentioning

confidence: 99%

Management of Landscapes for Established Invasive Species

Poland

Juzwik

Rowley

et al. 2021

Invasive Species in Forests and Rangelands of the United States

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Long-term management strategies are invoked once an invasive species has become established and spread beyond feasible limits for eradication or containment. Although an invasive species may be well-established in small to large geographical areas, prevention of its spread to non-affected areas (e.g., sites, regions, and cross-continent) through early detection and monitoring is an important management activity. The level for management of established invasive species in the United States has increasingly shifted to larger geographical scales in the past several decades. Management of an invasive fish may occur at the watershed level in the western States, with watershed levels defined by their hydrologic unit codes (HUC) ranging from 2 digits at the coarsest level to 8 digits at the finest level (USGS 2018). Invasive plant management within national forests, grasslands, and rangelands can be implemented at the landscape level (e.g., Chambers et al. 2014), although management can still occur at the stand or base level. Landscapes in this chapter refer to areas of land bounded by large-scale physiographic features integrated with natural or man-made features that govern weather and disturbance patterns and limit frequencies of species movement (Urban et al. 1987). These are often at a large physical scale, such as the Great Basin.

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Label-free detection of Phytophthora ramorum using surface-enhanced Raman spectroscopy

Cited by 40 publications

References 52 publications

Sandwiching analytes with structurally diverse plasmonic nanoparticles on paper substrates for surface enhanced Raman spectroscopy

Sandwiching analytes with structurally diverse plasmonic nanoparticles on paper substrates for surface enhanced Raman spectroscopy

TopUp Plasmonic Arrays for Surface‐Enhanced Raman Spectroscopy

Management of Landscapes for Established Invasive Species

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