Label-free DNA electrochemical sensor based on a PNA-functionalized conductive polymer

Reisberg, S.; Dang, Lan Anh; Nguyen, Que Anh S.; Piro, Benoı̂t; Noël, Vincent; Nielsen, P. E.; Le, Linda; Pham, Minh

doi:10.1016/j.talanta.2008.02.044

Cited by 55 publications

(25 citation statements)

References 42 publications

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“…An increase in the peak current of quinone was observed upon hybridisation of probes by the target DNAs, whereas no change is observed with noncomplementary sequence. Hybridisation was detected by recording the modification of the redox process of the quinone group, using SWV (Reisberg et al, 2008). Hashimoto et al have described the elaboration of electrochemical PNA arrays for the detection of the cancer gene ras; PNA was immobilised on gold electrodes of the array, and by using Linear Sweep Voltammetry (LSV) can provide a quick and convenient method for determining target DNAs specifically and quantitatively, with a detection limit of 10 -13 M (3 fmol) (Hashimoto, K. & Ishimori, Y.…”

Section: Voltametrymentioning

confidence: 99%

A Novel Type of Nucleic Acid-based Biosensors: the Use of PNA Probes, Associated with Surface Science and Electrochemical Detection Techniques

Mateo‐Martí¹,

Pradier²,

Jussieu³

2010

Intelligent and Biosensors

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Section: Voltametrymentioning

confidence: 99%

A Novel Type of Nucleic Acid-based Biosensors: the Use of PNA Probes, Associated with Surface Science and Electrochemical Detection Techniques

Mateo‐Martí¹,

Pradier²,

Jussieu³

2010

Intelligent and Biosensors

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“…Jadranka Travas-Sejdic [27] et al present a label-free sensor based on poly [pyrrole-co-4-(3-pyrrolyl) butanoic acid]. M. C. Pham [30] et al report a DNA hybridization biosensor based on poly(JUG-co-JUGA) films modified electrode. S. S. Zhang [31] et al report a label-free electrochemical DNA sensor based on poly (indole-5-carboxylic acid) conducting polymer.…”

Section: Introductionmentioning

confidence: 99%

Lable‐Free Electrochemical DNA Sensor Based on Gold Nanoparticles/Poly(neutral red) Modified Electrode

Zhang

2010

Electroanalysis

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We present a new strategy for the label-free electrochemical detection of DNA hybridization based on gold nanoparticles (AuNPs)/poly(neutral red) (PNR) modified electrode. Probe oligonucledotides with thiol groups at the 5-end were covalently linked onto the surface of AuNPs/PNR modified electrode via S-Au binding. The hybridization event was monitored by using differential pulse voltammetry (DPV) upon hybridization generates electrochemical changes at the PNR-solution interface. A significant decrease in the peak current was observed upon hybridization of probe with complementary target ssDNA, whereas no obvious change was observed with noncomplementary target ssDNA. And the DNA sensor also showed a high selectivity for detecting one-mismatched and three-mismatched target ssDNA and a high sensitivity for detecting complementary target ssDNA, the detection limit is 4.2 Â 10 À12 M for complementary target ssDNA. In addition, the DNA biosensor showed an excellent reproducibility and stability under the DNA-hybridization conditions.

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“…The hybridization response of the PNA biosensor is independent of the ionic strength and hybridization temperature over 1-20 mM phosphate buffer concentration and 22-50 8C, respectively with detection limit of 10 pmol of target within 10 minutes [11][12][13]. Many materials like CPE [14], self-assembled manolayers, [15], streptavidin coated super magnetic iron oxide beads [16], quinone-based electroactive polymer [17], gold electrodes [18,19] Si/SiO 2 chips [20], silicon nanowire (SiNW) [21] and conducting polymers like polypyrrole (PPy) and polyaniline (PANI) etc. have recently been explored for development of electrochemical PNA sensors.…”

Section: Introductionmentioning

confidence: 99%

Peptide Nucleic Acid Immobilized Biocompatible Silane Nanocomposite Platform for Mycobacterium tuberculosis Detection

et al. 2010

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We have prepared nanocomposite films comprising of 3-glycidoxypropyltrimethoxysilane (GOPS) and iron-oxide (Fe 3 O 4 ) onto indium-tin-oxide (ITO) glass plate for covalent immobilization of 21-mer peptide nucleic acid (PNA). These films have been characterized using contact angle, atomic force microscopy (AFM), electrochemical techniques. The electrochemical response of the GOPS/ITO and Fe 3 O 4 -GOPS/ITO electrodes has been investigated by hybridization with complementary, non-complementary and one-base mismatch using methylene blue as electrochemical indicator. The PNA/Fe 3 O 4 -GOPS/ITO bioelectrode exhibits improved specificity and detection limit (0.1 fM) as compared to that of the PNA-GOPS/ITO bioelectrode (0.1 pM). This PNA/Fe 3 O 4 -GOPS/ITO electrode can be utilized for detection of hybridization with the complementary sequence in sonicated M. tuberculosis genomic DNA within 90 s of hybridization time.

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Label-free DNA electrochemical sensor based on a PNA-functionalized conductive polymer

Cited by 55 publications

References 42 publications

A Novel Type of Nucleic Acid-based Biosensors: the Use of PNA Probes, Associated with Surface Science and Electrochemical Detection Techniques

A Novel Type of Nucleic Acid-based Biosensors: the Use of PNA Probes, Associated with Surface Science and Electrochemical Detection Techniques

Lable‐Free Electrochemical DNA Sensor Based on Gold Nanoparticles/Poly(neutral red) Modified Electrode

Peptide Nucleic Acid Immobilized Biocompatible Silane Nanocomposite Platform for Mycobacterium tuberculosis Detection

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