Label-free electrical detection of pyrophosphate generated from DNA polymerase reactions on field-effect devices

Credo, Grace M.; Su, Xing; Wu, Kai; Elibol, Oğuz H.; Liu, Jianquan; Reddy, Bobby; Tsai, Ta Wei; Dorvel, Brian; Daniels, John R.; Bashir, Rashid; Varma, Madoo

doi:10.1039/c2an15930a

Cited by 31 publications

(21 citation statements)

References 40 publications

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“…Specifically, for fluorescent molecular imaging, FITC was chemically modified on one end of the RCR primer, such that multiple copies of fluorophores could be integrated into one NF composed of many copies of DNA building blocks. Bioimaging agents were incorporated enzymatically via chemically modified deoxynucleotides, a Cyanine 5 (Cy5)-modified dUTP, which can work as a substrate for many DNA polymerases, including Φ29 [117,118]. The fluorescence of these resultant NFs was validated through fluorescence microscopy.…”

Section: Aptamer-tethered Dna Nanotrains For Targeted Delivery Of Molmentioning

confidence: 99%

Aptamers-Guided DNA Nanomedicine for Cancer Theranostics

Zhu

Qiu

Meng

et al. 2015

Aptamers Selected by Cell-Selex for Theranostics

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The past two decades have witnessed the booming of DNA aptamers, and particularly the development of aptamers for the specific recognition of versatile disease-related molecular biomarkers and living cells, as well as the application of aptamers for molecular and cellular engineering, bioanalysis, and disease therapy. Owing to the predictable Watson-Crick base-pairing, DNA can be easily designed and engineered to construct sophisticated molecular devices and nanostructures, which, at the same time, can be integrated with many biofunctionalities, including DNA aptamers for specific target recognition, bioimaging agents for biosensing, as well as drug-loading moieties for targeted drug delivery. The ability of many aptamers to mediate internalization into mammalian cells additionally empowered aptamer-incorporated DNA devices to be utilized for intracellular delivery of biosensors and drug carriers, and eventually sensing intracellular biomolecular behaviors in real-time or modulating intracellular biological activities for therapeutic purposes. In this chapter, we discuss the development of aptamerintegrated DNA nanodevices for versatile applications in bioanalysis and disease therapy, with an emphasis on cancer theranostics.

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Section: Aptamer-tethered Dna Nanotrains For Targeted Delivery Of Molmentioning

confidence: 99%

Aptamers-Guided DNA Nanomedicine for Cancer Theranostics

Zhu

Qiu

Meng

et al. 2015

Aptamers Selected by Cell-Selex for Theranostics

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“…Because of the phosphate recognizing ability of the Zn II -dpa complexes [82], the electrical detection of pyrophosphate (PPi) was achieved on the Zn II -dpa-treated FET (Figure 13d). Based on the ability of the FET, prepared as above, to sense PPi, the electrical monitoring of DNA polymerase reactions was successfully demonstrated using the same device [50]. Interestingly, the slight changes in the molecular structures of phosphate anions were discriminated by the Zn II -dpa SAM [51].…”

Section: Electrochemical/electrical Detection Of Analytes For the Achmentioning

confidence: 99%

The Power of Assemblies at Interfaces: Nanosensor Platforms Based on Synthetic Receptor Membranes

Minamiki

Ichikawa

Kurita

2020

Sensors

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Synthetic sensing materials (artificial receptors) are some of the most attractive components of chemical/biosensors because of their long-term stability and low cost of production. However, the strategy for the practical design of these materials toward specific molecular recognition in water is not established yet. For the construction of artificial material-based chemical/biosensors, the bottom-up assembly of these materials is one of the effective methods. This is because the driving forces of molecular recognition on the receptors could be enhanced by the integration of such kinds of materials at the 'interfaces', such as the boundary portion between the liquid and solid phases. Additionally, the molecular assembly of such self-assembled monolayers (SAMs) can easily be installed in transducer devices. Thus, we believe that nanosensor platforms that consist of synthetic receptor membranes on the transducer surfaces can be applied to powerful tools for high-throughput analyses of the required targets. In this review, we briefly summarize a comprehensive overview that includes the preparation techniques for molecular assemblies, the characterization methods of the interfaces, and a few examples of receptor assembly-based chemical/biosensing platforms on each transduction mechanism.Synthetic receptors (i.e., artificial molecular recognition materials) are some of the most suitable platform materials for the development of chemical/biosensors owing to their chemical/physical stability, low cost of production, and their fine-tuning ability to select the required targets [4]. However, the preparation of artificial receptor-based sensors for practical applications is still in its initial stages, since the analyte specificity of most of the artificial materials is generally lower than that of biomaterials (i.e., antibodies and enzymes) [5]. To improve the binding affinity between the artificial receptors and the analytes, complicated synthesis procedures are required. Hence, a simpler way to improve the sensing ability of the artificial receptors should be considered to bring out the attractive features of these materials. To facilitate this, bottom-up integration of the receptors as molecular assemblies is considered as one of the most useful approaches for the construction of selective sensing fields for analytes. Moreover, the sensing features in the artificial receptor-based sensors could be finely tuned by using top-down technologies (e.g., molecular imprinting techniques [6], etc.) because the molecular recognition ability of the receptor assemblies depends on their nanostructures [7]. As aforementioned, the molecular assembly enhances the functions of a single kind of molecule ( Figure 1) [8]. In addition, the driving forces in molecular recognition (i.e., noncovalent interactions) can be amplified at the interfaces such as the boundary portion between the liquid and solid phases [9,10]. Thus, we believe that the installation of artificial receptors at the surface of the sensing portion in selective transducer...

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“…Broad interest in DNA biosensors and their growing applications are connected with their ability to perform fast, sensitive, and selective detection of hybridization events between a DNA probe and its complementary target molecule. Numerous DNA biosensors have been developed based on various signal generation principles such as optical , electro‐chemical , and polymerase‐based sensors . However, most of the DNA detection techniques usually require the careful designing and labeling of DNA molecules, and therefore, have been proved to be complicated, time‐consuming, and costly.…”

Section: Introductionmentioning

confidence: 99%

Label‐free electrical detection of DNA with a multi‐spot LAPS: First step towards light‐addressable DNA chips

Bronder

Poghossian

et al. 2014

Physica Status Solidi (a)

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6009 53235A multi-spot (4 Â 4 spots) light-addressable potentiometric sensor (MLAPS) consisting of an Al-p-Si-SiO 2 structure has been applied for the label-free electrical detection of DNA (deoxyribonucleic acid) immobilization and hybridization by the intrinsic molecular charge for the first time. Single-stranded probe ssDNA molecules (20 bases) were covalently immobilized onto the silanized SiO 2 gate surface. The unspecific adsorption of mismatch ssDNA on the MLAPS gate surface was blocked by bovine serum albumin molecules. To reduce the screening effect and to achieve a high sensor signal, the measurements were performed in a low ionic-strength solution. The photocurrent-voltage (I-V) curves were simultaneously recorded on all 16 spots after each surface functionalization step. Large shifts of I-V curves of 25 mV were registered after the DNA immobilization and hybridization event. In contrast, a small potential shift ($5 mV) was observed in case of mismatch ssDNA, revealing good specificity of the sensor. The obtained results demonstrate the potential of the MLAPS as promising transducer platform for the multi-spot label-free electrical detection of DNA molecules by their intrinsic molecular charge.

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Label-free electrical detection of pyrophosphate generated from DNA polymerase reactions on field-effect devices

Cited by 31 publications

References 40 publications

Aptamers-Guided DNA Nanomedicine for Cancer Theranostics

Aptamers-Guided DNA Nanomedicine for Cancer Theranostics

The Power of Assemblies at Interfaces: Nanosensor Platforms Based on Synthetic Receptor Membranes

Label‐free electrical detection of DNA with a multi‐spot LAPS: First step towards light‐addressable DNA chips

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