Label-free electrochemical detection of human methyltransferase from tumors

Furst, Ariel L.; Muren, Natalie B.; Hill, Michael G.; Barton, Jacqueline K.

doi:10.1073/pnas.1417351111

Cited by 73 publications

(93 citation statements)

References 34 publications

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“…About four different categories of this enzyme were identified in mammals namely DNMT1, DNMT2, DNMT3A, and DNMT3B. 2,7,11,18 It was suggested that SNPs in the genes responsible for the expression of these enzymes perhaps affect their role in methylation of mutant genes. Consequently, the role of mutant genes in the pathogenesis of cancer may be altered.…”

Section: Epigenetics and Sources Methyl Groupmentioning

confidence: 99%

“…Consequently, the role of mutant genes in the pathogenesis of cancer may be altered. 2,11,13,18,21 In other words, genetic polymorphism of DNMT enzymes may affect their role in epigenetic modifications via gene methylation. Therefore, genetic polymorphisms and epigenetic variations may contribute simultaneously to the aetiology of cancer.…”

Section: Epigenetics and Sources Methyl Groupmentioning

confidence: 99%

“…Therefore, genetic polymorphisms and epigenetic variations may contribute simultaneously to the aetiology of cancer. 2,11,13,18,21 The progress of cancer depends on whether the oncogenes or cancer suppressing genes is methylated, or their methylation is inhibited and vice versa. In addition, cancer and autoimmune disease are believed to occur when there is hypermethylation of promoter CPG linked bases and hypomethylation of another region of the genome.…”

Section: Epigenetics and Sources Methyl Groupmentioning

confidence: 99%

“…2,8,13 During cell division, methionine from diet supply methyl group that attached to the pair of the chromosome at CytosinePhosphateGuanine (CPG) axis by enzyme DNMT. 1,2,7,8,13,[18][19] At this point, the methylated chromosome strand attached itself to the unmethylated strand to form pairs. The DNMT enzyme subsequently adds a methyl group to the unmethylated CPG Island in a daughter DNA.…”

mentioning

confidence: 99%

“…The DNMT enzyme subsequently adds a methyl group to the unmethylated CPG Island in a daughter DNA. 1,2,7,8,13,18,19 The daughter DNA usually winds itself around a histone protein to produce chromatin. Processes such as methylation, acetylation, phosphorylation, ubiquitination or sumoylation of chromatin around histone may give rise to epigenetic changes.…”

mentioning

confidence: 99%

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Epigenetics: Impacts on Disease Susceptibility and Pharmacotherapy

Abubakar¹,

Haque²

2016

IJPER

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Deoxyribonucleic acid (DNA) is a hereditary material comprising of all the genetic information in humans and almost all other living creatures. It is made up of subunits called genes, which have four nucleotides. MicroRNAs comprises of the short noncoding RNA. They are non-protein coding with about 18-22 nucleotides which control gene expression and activities post-transcriptionally. Notably, the whole DNA structure usually coiled itself around a protein called histone moiety. Consequently, any addition of methyl, ethyl, phosphate, Ubiquitin or small ubiquitin-related modifier either directly on the DNA itself or the histone molecule will ultimately change its configuration and stability leading to the epigenetic modifications. Usually, epigenetic changes manifest phenotypically without changing the actual gene sequence. MicroRNAs also play a vital role in the control of the expression epigenetic regulators, such as DNA methyltransferase (DNMT), histone deacetylase, and Polycomb group genes. The implication of this may significantly affect the normal function of an organism and perhaps contribute to the wide-range of disease aetiology. As such, epigenetics linked variations and diseases are inheritable. The landmark achievement in pharmacotherapy involves the possibility of silencing the oncogenes that are cancer provoking genes and possible reactivation of cancer suppression genes that battle with and repress the tumour cells proliferation. Besides these, more genes that are linked to other diseases apart from cancer can be identified, silenced or activated using drugs or diets in order to treat the disease aetiology. This appraisal is to discuss the role of epigenetic changes in the disease susceptibility and drug treatment.

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Section: Epigenetics and Sources Methyl Groupmentioning

confidence: 99%

Section: Epigenetics and Sources Methyl Groupmentioning

confidence: 99%

Section: Epigenetics and Sources Methyl Groupmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Epigenetics: Impacts on Disease Susceptibility and Pharmacotherapy

Abubakar¹,

Haque²

2016

IJPER

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Nanostructured Architectures for Biomolecular Detection inside and outside the Cell

Chen

Yousefi

Nemr

et al. 2019

Adv Funct Materials

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Nanostructured materials can now be engineered with great precision and complexity as a result of advances in design and fabrication, and offer distinct advantages in many biosensing and biomedical applications. The materials most widely used in this field are semiconductors and noble metals. Each offers multiple length scales of nanostructuring that program their physicochemical properties for different biosensing applications. Here, nanostructured materials and their applications are reviewed together with semiconductors and noble metals, as well as hybrid materials that unite these two classes—all with the goal of linking performance characteristics to applications in biomedicine.

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Rapid Electrochemical Assessment of Tumor Suppressor Gene Methylations in Raw Human Serum and Tumor Cells and Tissues Using Immunomagnetic Beads and Selective DNA Hybridization

et al. 2018

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We report a rapid and sensitive electrochemical strategy for the detection of gene‐specific 5‐methylcytosine DNA methylation. Magnetic beads (MBs) modified with an antibody for 5‐methylcytosines (5‐mC) are used for the capture of any 5‐mC methylated single‐stranded (ss)DNA sequence. A flanking region next to the 5‐mCs of the captured methylated ssDNA is recognized by hybridization with a synthetic biotinylated DNA sequence. Amperometric transduction at disposable screen‐printed carbon electrodes (SPCEs) is employed. The developed biosensor has a dynamic range from 3.9 to 500 pm and a limit of detection of 1.2 pm for the methylated synthetic sequence of the tumor suppressor gene O‐6‐methylguanine‐DNA methyltransferase (MGMT) promoter region. The method is applied in the 45‐min analysis of specific methylation in the MGMT promoter region directly in raw spiked human serum samples and in genomic DNA extracted from U‐87 glioblastoma cells and paraffin‐embedded brain tumor tissues without any amplification and pretreatment step.

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Label-free electrochemical detection of human methyltransferase from tumors

Cited by 73 publications

References 34 publications

Epigenetics: Impacts on Disease Susceptibility and Pharmacotherapy

Epigenetics: Impacts on Disease Susceptibility and Pharmacotherapy

Nanostructured Architectures for Biomolecular Detection inside and outside the Cell

Rapid Electrochemical Assessment of Tumor Suppressor Gene Methylations in Raw Human Serum and Tumor Cells and Tissues Using Immunomagnetic Beads and Selective DNA Hybridization

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