Label-free electrochemical detection of methyltransferase activity and inhibitor screening based on endonuclease HpaII and the deposition of polyaniline

Zhang, Linqun; Wei, Min; Gao, Chunyan; Wei, Wei; Zhang, Hongjie; Liu, Songqin

doi:10.1016/j.bios.2015.05.066

Cited by 39 publications

(18 citation statements)

References 51 publications

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“…DNA methylation and methyltransferase activity, which are linked to the development of cancer, have recently been detected electrochemically in human serum samples in the range of 0.5–0.6 U ml −1 with an LOD of 0.12 U ml −1 (Zhang et al 2015b). It is based on methylation sensitive restriction endonuclease Hpa II and the DNAzyme-catalyzed polymerization of aniline.…”

Section: Applicationsmentioning

confidence: 99%

Enzymatic oligomerization and polymerization of arylamines: state of the art and perspectives

et al. 2016

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The literature concerning the oxidative oligomerization and polymerization of various arylamines, e.g., aniline, substituted anilines, aminonaphthalene and its derivatives, catalyzed by oxidoreductases, such as laccases and peroxidases, in aqueous, organic, and mixed aqueous organic monophasic or biphasic media, is reviewed. An overview of template-free as well as template-assisted enzymatic syntheses of oligomers and polymers of arylamines is given. Special attention is paid to mechanistic aspects of these biocatalytic processes. Because of the nontoxicity of oxidoreductases and their high catalytic efficiency, as well as high selectivity of enzymatic oligomerizations/polymerizations under mild conditions—using mainly water as a solvent and often resulting in minimal byproduct formation—enzymatic oligomerizations and polymerizations of arylamines are environmentally friendly and significantly contribute to a “green” chemistry of conducting and redox-active oligomers and polymers. Current and potential future applications of enzymatic polymerization processes and enzymatically synthesized oligo/polyarylamines are discussed.

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Section: Applicationsmentioning

confidence: 99%

Enzymatic oligomerization and polymerization of arylamines: state of the art and perspectives

et al. 2016

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“…The inset plot revealed a good linear relationship between DPV intensity and the logarithm of the concentration of M.SssI MTase in the range of 0.05e10 U mL À1 with the linear correlation coefficient of 0.994. The detection limit (LOD) was calculated to be 0.03 U mL À1 (in terms of the rule of 3 times standard deviation over the blank response), which is much lower than our previously reported method [30] where the DPV response of RuHex amplified by mimic-HCR was over ten times higher than that of polyaniline based only on DNAzyme. The comparison of various analytic performances was list in Table 2.…”

Section: Detection Of Msssi Mtase Activity By Using the Sensormentioning

confidence: 98%

“…Ai recently employed a new type of DNA functionalized nano/mesoporous silica to detect DNA MTase activity with G-quadruplex as a lock for electrochemical signal molecule releasing [31]. Our group designed an electrochemical method for sensitive detection of DNA methylation and MTase activity based on the deposition of polyaniline catalyzed by HRP-mimicking DNAzyme [30].…”

Section: Introductionmentioning

confidence: 99%

Sensitive electrochemical assaying of DNA methyltransferase activity based on mimic-hybridization chain reaction amplified strategy

Zhang

Liu

et al. 2016

Analytica Chimica Acta

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“…18 Furthermore, in order to simplify the process, shorten the analysis time, improve the sensitivity, and lower the cost, many new techniques and methods such as fluorescence methods based on hairpin DNA probe, [19][20][21] enzyme immunoassay based on gold nanoparticles, 22,23 surface-enhanced Raman spectroscopy, 24,25 chemiluminescence assay, 26,27 electrochemical methods, [28][29][30][31] and so on have been designed to evaluate the activity of DNA MTase.…”

Section: Yu Et Almentioning

confidence: 99%

Magnetic immunoassay using CdSe/ZnS quantum dots as fluorescent probes to detect the level of DNA methyltransferase 1 in human serum sample

Yu¹,

Xiong²,

Yu³

et al. 2018

IJN

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Background DNA methyltransferase 1 (DNMT1), a dominant enzyme responsible for the transfer of a methyl group from the universal methyl donor to the 5-position of cytosine residues in DNA, is essential for mammalian development and closely related to cancer and a variety of age-related chronic diseases. DNMT1 has become a useful biomarker in early disease diagnosis and a potential therapeutic target in cancer therapy and drug development. However, till now, most of the studies on DNA methyltransferase (MTase) detection have focused on the prokaryote MTase and its activity. Methods A magnetic fluorescence-linked immunosorbent assay (FLISA) using CdSe/ZnS quantum dots as fluorescent probes was proposed for the rapid and sensitive detection of the DNMT1 level in this study. Key factors that affect the precision and accuracy of the determination of DNMT1 were optimized. Results Under the optimal conditions, the limit of detection was 0.1 ng/mL, the linear range was 0.1–1,500 ng/mL, the recovery was 91.67%–106.50%, and the relative standard deviations of intra- and inter-assays were respectively 5.45%–11.29% and 7.03%–11.25%. The cross-reactivity rates with DNA methyltransferases 3a and 3b were only 4.0% and 9.4%, respectively. Furthermore, FLISA was successfully used to detect the levels of DNMT1 in human serum samples, and compared with commercial enzyme-linked immunosorbent assay (ELISA) kits. The results revealed that there was a good correlation between FLISA and commercial ELISA kits (correlation coefficient r =0.866, p =0.001). The linear scope of FLISA was broader than ELISA, and the measurement time was much shorter than ELISA kits. Conclusion These indicated that the proposed FLISA method was sensitive and high throughput and can quickly screen the level of DNMT1 in serum samples.

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Label-free electrochemical detection of methyltransferase activity and inhibitor screening based on endonuclease HpaII and the deposition of polyaniline

Cited by 39 publications

References 51 publications

Enzymatic oligomerization and polymerization of arylamines: state of the art and perspectives

Enzymatic oligomerization and polymerization of arylamines: state of the art and perspectives

Sensitive electrochemical assaying of DNA methyltransferase activity based on mimic-hybridization chain reaction amplified strategy

Magnetic immunoassay using CdSe/ZnS quantum dots as fluorescent probes to detect the level of DNA methyltransferase 1 in human serum sample

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