Label‐Free Electrochemical Recognition of DNA Hybridization by Means of Modulation of the Feedback Current in SECM

Turcu, Florin; Schulte, Albert; Hartwich, Gerhard; Schuhmann, Wolfgang

doi:10.1002/anie.200454228

Cited by 78 publications

(51 citation statements)

References 37 publications

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“…) and the negatively charged phosphate backbone of DNA was identified as the source for the observed changes mainly in the charge transfer resistance which was confirmed by means of scanning electrochemical microscopy [18,19] In this communication, EIS for the detection of DNA hybridization is complemented by the incorporation of intercalating compounds into the helix of dsDNA which is seen as an unambiguous additional evidence for complementary DNA hybridization. Actinomycin D (ActD) and proflavine are known as antibiotics and they exhibit high affinity to dsDNA.…”

Section: Introductionmentioning

confidence: 90%

Label‐Free Detection of DNA Hybridization in Presence of Intercalators Using Electrochemical Impedance Spectroscopy

et al. 2009

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Actinomycin D or proflavine which are known to intercalate within the helix of double-stranded DNA (dsDNA) are used as label-free control to unequivocally prove complementary DNA hybridization by means of electrochemical impedance spectroscopy (EIS). Based on a carefully designed interface comprising a thiol-tethered (20mer) oligonucleotide capture probe which forms a self-assembled monolayer on a gold electrode together with a short chain hydroxyl-terminated alkylthiol, formation of dsDNA can be monitored by an increase of the charge-transfer resistance of a free-diffusing negatively charged redox species ([Fe(CN) 6 ] 3À/4À ). The increase of the charge transfer resistance due to complementary hybridization was about 10 times from the unmodified Au surface to the dsDNA modified electrode. Specific interaction of intercalators with dsDNA leads to a decrease in charge transfer resistance due to the conformational changes in the dsDNA monolayer and partial charge compensation caused by the positively charged intercalators. No shift in the charge transfer resistance was observed in case of incubation of a ssDNA surface with intercalators or when hybridization was invoked using a noncomplementary DNA sequence. Thus, hybridization can be unambiguously detected using EIS by first recording the increase in charge-transfer resistance due to hybridization with the matching target strand followed by recording a decrease in charge transfer resistance caused by intercalation. Nonspecific adsorption can hence be doubtlessly excluded as a reason for the observed changes in the impedance spectrum.

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Section: Introductionmentioning

confidence: 90%

Label‐Free Detection of DNA Hybridization in Presence of Intercalators Using Electrochemical Impedance Spectroscopy

et al. 2009

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“…9). 39,40 This group used Fe(CN) 6 3 as a mediator, which can report changes in the surface-charge status. The sample DNA was a 20-mer synthetic oligonucleotide and the DNA molecules were attached as 150-μm-diameter dots through standard 5'-modified chemisorption on gold-covered glass slides.…”

Section: Examples Of Negative Feedback Mode Imagingmentioning

confidence: 99%

3 Scanning Electrochemical Microscopy Imaging of DNA Arrays for High Throughput Analysis Applications

Nakano

2011

Modern Aspects of Electrochemistry

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“…Turcu et al [31] introduced an electrostatic approach, exploiting charge repulsion, to visualize the surface-bound DNA hybridization process. The approach involves exploiting the Coulombic interactions between a negatively charged solution-based redox mediator, [Fe(CN) 6 ] 4À , and the backbone phosphodiester groups of the immobilized DNA strands as shown in Fig.…”

Section: Multiplexed Dna Visualization Of Hybridization and Mismatch mentioning

confidence: 99%

Scanning Electrochemical Microscopy: A Multiplexing Tool for Electrochemical DNA Biosensing

Shamsi¹,

Kraatz²

2015

Handbook of Nanoelectrochemistry

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Scanning electrochemical microscopy (SECM) translates the current generated by an electrochemical reaction occurring at a tip electrode scanned across a surface substrate into an image. SECM not only provides a simple electrochemical image of the conductive and/or insulating substrate but also provides kinetic information of the heterogeneous electron transfer reactions when the tip electrode approaches the surface. Applications including biosensing have been demonstrated. In this chapter, we will focus on recent advances in the application of SECM toward the label-free detection of base pair mismatches in DNA.Despite having nanometer dimensions, the base pair mismatches along a DNA strand can be readily detected by SECM in an array format through exploiting the negative charge in the vicinity of selfassembled DNA films. The response can be amplified using metal ions to enhance the discrimination between matched and mismatched DNA films. This simple strategy has been used to probe the position of a single nucleotide mismatch, the type of the mismatch, and hybridization position of complementary strand and even allows the identification of various animal species.

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Label‐Free Electrochemical Recognition of DNA Hybridization by Means of Modulation of the Feedback Current in SECM

Cited by 78 publications

References 37 publications

Label‐Free Detection of DNA Hybridization in Presence of Intercalators Using Electrochemical Impedance Spectroscopy

Label‐Free Detection of DNA Hybridization in Presence of Intercalators Using Electrochemical Impedance Spectroscopy

3 Scanning Electrochemical Microscopy Imaging of DNA Arrays for High Throughput Analysis Applications

Scanning Electrochemical Microscopy: A Multiplexing Tool for Electrochemical DNA Biosensing

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