Label-free fluorescence “turn-on” detection of SO<sub>3</sub><sup>2−</sup> by gold nanoclusters: integration in a hydrogel platform and intracellular detection

Sachdev, Abhay; Raj, R. Mohan; Matai, Ishita; Kumar, Vinay; Gopinath, P.; Mishra, Sunita

doi:10.1039/c8ay02813c

Cited by 15 publications

(6 citation statements)

References 56 publications

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“…As for the PCuNCs-BCuNCs, combined observation concerning the presence of distinctive absorption peaks at 278 nm (PCuNCs) and a shoulder peak at 310 nm (BCuNCs) along with shift in the absorption peak to longer wavelength indicated the formation of the hybrid nanoprobe [46][47][48][49]. The absence of surface plasmon resonance peak at 560 nm further eliminates the possibility of formation of large Cu nanoparticles in the nanoprobe or bulk metal by-products [48][49][50][51]. Further, the fluorescence response of the nanoprobe was also examined (Fig.…”

Section: Preparation and Characterizationmentioning

confidence: 97%

Dual-emission copper nanoclusters–based ratiometric fluorescent probe for intracellular detection of hydroxyl and superoxide anion species

et al. 2021

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A fluorescent nanoprobe based on copper nanoclusters (CuNCs) has been developed for ratiometric detection of hydroxyl radicals (• OH) and superoxide anion radicals (O 2 •−). Two differently luminescent CuNCs, namely cyan-emissive poly(methacrylic acid)-protected copper nanoclusters (PCuNCs) and orange-emissive bovine serum albumin-protected CuNCs (BCuNCs), were conjugated to obtain a hybrid, dual-emission nanoprobe (PCuNCs-BCuNCs) with the corresponding peaks at 445 nm and 652 nm at an excitation wavelength of 360 nm. In particular, the fluorescence peak at 445 nm gradually enhanced with the incremental addition of • OH and O 2 •−. However, the fluorescence emission at 652 nm was greatly quenched in the presence of • OH, while in case of O 2 •− , the fluorescence intensity remained constant. The differential response of the PCuNCs-BCuNCs towards • OH and O 2 •− formed the basis of ratiometric detection. Under optimal conditions, the PCuNCs-BCuNCs exhibited good sensitivity and linearity towards • OH and O 2 •− with limits of detection of 0.15 μM and 1.8 μM, respectively. Moreover, the nanoprobe exhibited high selectivity for • OH and O 2 •− over other potential ROS interferences. Besides, PCuNCs-BCuNCs were eventually applied for qualitative and quantitative ratiometric assessment of intracellular • OH and O 2 •− in L-132 cells. Therefore, this strategy unveils a new potential for copper nanocluster-based sensing of ROS.

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Section: Preparation and Characterizationmentioning

confidence: 97%

Dual-emission copper nanoclusters–based ratiometric fluorescent probe for intracellular detection of hydroxyl and superoxide anion species

et al. 2021

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“…Au, as one of the most stable elements in the periodic table, is extensively studied for its diverse properties when exhibited in different nanoforms. Au nanoclusters (Au NCs) around 3 nm in size exhibit a range of properties, including catalytic performance, optical characteristics, biocompatibility, bioactivity, and surface functionalization. − The surface of Au NCs coated with different amounts of protein can inhibit the peroxidase ability to different degrees, and this has been applied to design a multicolor sensor for trypsin . A noteworthy observation is that the catalytic activity of Au nanoparticles, loaded with ruthenium catalysts, was found to be inhibited by biomolecule-induced aggregation in a protein-rich 1% serum environment.…”

Section: Introductionmentioning

confidence: 99%

Multicolor Biosensor of Hyaluronidase by the Regulation of Enzyme-Like Catalytic Activity of Au Nanoclusters through Changes in the Aggregation State

Tian,

Lin,

Lin

et al. 2024

ACS Appl. Nano Mater.

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The control of nanoparticle morphology can effectively change their properties. Adjusting the aggregation state of bovine serum albumin-coated gold nanoclusters (BSA-Au NCs) can regulate the enzyme-mimicking catalytic activity. Hyaluronic acid (HA) induces the aggregation of BSA-Au NCs, leading to the shielding of the catalytic active sites and a decrease in activity; this feature has been utilized to design a multicolor biosensor for hyaluronidase (HAase). The presence of HAase can hydrolyze the glycosidic bonds in HA, causing the aggregated BSA-Au NCs to disperse and express their catalytic activity, which in turn catalyzes the etching of Au nanobipyramids (Au-NBPs) in the presence of 3,3′,5,5′-tetramethylbenzidine (TMB), resulting in a morphological transition from bipyramidal to ellipsoidal and spherical shapes, while the solution displays a variety of colors. Visual recognition of these multicolor changes establishes a relationship between the enzyme activity and color. Based on the controlled aggregation and dispersion of BSA-Au NCs and the etching of Au-NBPs, a simple multicolor HAase biosensor was designed. The proposed biosensor shows a linear response to HAase concentrations in the range of 5−80 U/mL, with clear color changes under optimized conditions. The limit of detection (LOD) of the sensor was determined to be 1.98 U/mL (LOD = 3s/k). This method successfully applies the changing of enzyme-mimicking catalytic activity nanoparticles for colorimetric analysis detection.

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“…Thus, the domains of nanodevices, biomaterials, drug transport and release, chemical sensors, catalyst carriers, gas adsorption, and storage showed promising prospects for the application of supramolecular hydrogels. [23][24][25][26][27][28][29] Additionally, supramolecular hydrogels can serve as an easy framework for the development of multifunctional solid support fluorescent materials.…”

Section: Introductionmentioning

confidence: 99%

Luminogenic and Bactericidal Studies of an Acrylonitrile‐Based AIEgen

De,

Ghosh,

Das

2024

Chemistry An Asian Journal

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We have synthesized an aggregation induced emissive molecule that exhibits promising photophysical characteristics. The aggregating aptitude is demonstrated by binary solvent mixture and it is emissive in both solution and solid state. The luminogenic characteristics are employed in creating fluorescent inks as well as for the detection of nitro antibiotics in biofluids and in solid support. Moreover, the acrylonitrile‐based compound is bactericidal tested on E. coli and B. subtilis.

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Label-free fluorescence “turn-on” detection of SO₃²⁻ by gold nanoclusters: integration in a hydrogel platform and intracellular detection

Abstract: A new dimension for the fluorescence “turn-on” detection of SO32− by Au NCs with potential applications towards the development of a hydrogel based platform and intracellular detection.

Cited by 15 publications

References 56 publications

Dual-emission copper nanoclusters–based ratiometric fluorescent probe for intracellular detection of hydroxyl and superoxide anion species

Dual-emission copper nanoclusters–based ratiometric fluorescent probe for intracellular detection of hydroxyl and superoxide anion species

Multicolor Biosensor of Hyaluronidase by the Regulation of Enzyme-Like Catalytic Activity of Au Nanoclusters through Changes in the Aggregation State

Luminogenic and Bactericidal Studies of an Acrylonitrile‐Based AIEgen

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Label-free fluorescence “turn-on” detection of SO32− by gold nanoclusters: integration in a hydrogel platform and intracellular detection

Abstract: A new dimension for the fluorescence “turn-on” detection of SO32− by Au NCs with potential applications towards the development of a hydrogel based platform and intracellular detection.

Cited by 15 publications

References 56 publications

Dual-emission copper nanoclusters–based ratiometric fluorescent probe for intracellular detection of hydroxyl and superoxide anion species

Dual-emission copper nanoclusters–based ratiometric fluorescent probe for intracellular detection of hydroxyl and superoxide anion species

Multicolor Biosensor of Hyaluronidase by the Regulation of Enzyme-Like Catalytic Activity of Au Nanoclusters through Changes in the Aggregation State

Luminogenic and Bactericidal Studies of an Acrylonitrile‐Based AIEgen

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Label-free fluorescence “turn-on” detection of SO₃²⁻ by gold nanoclusters: integration in a hydrogel platform and intracellular detection