Abstract:Spore-forming bacteria accumulate dipicolinic acid (DPA) to form spores to survive in extreme environments. Vibrational spectroscopy is widely used to detect DPA to elucidate the existence of the bacteria, while a vegetative cell, another form of spore-forming bacteria, has not studied deeply. Here we applied coherent anti-Stokes Raman scattering (CARS) microscopy to spectroscopically identify both spores and vegetative cells without staining or molecular tagging. The spores were identified by the strong CARS … Show more
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