Label-free imaging and biomarker analysis of exosomes with plasmonic scattering microscopy

Zhang, Pengfei; Jiang, Jiapei; Zhou, Xinyu; Kolay, Jayeeta; Wang, Rui; Wan, Zijian; Wang, Shaopeng

doi:10.1039/d2sc05191e

Cited by 30 publications

(53 citation statements)

References 54 publications

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“…S2 †). 19,20,[30][31][32] The lateral resolution of the ESM is limited by the optical diffraction, which is ∼0.6 μm in this work. Besides, different from the PSM, the ESM employs the cover glasses, which own much smaller surface roughness and is more friendly for the cell adhesion than gold-coated cover glasses in PSM, as the sensor chips, thus allowing clear observations of cell adhesion sites (Fig.…”

Section: Detection Principlesmentioning

confidence: 99%

Label-free analysis of membrane protein binding kinetics and cell adhesions using evanescent scattering microscopy

Xu,

Huang,

et al. 2023

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Section: Detection Principlesmentioning

confidence: 99%

Label-free analysis of membrane protein binding kinetics and cell adhesions using evanescent scattering microscopy

Xu,

Huang,

et al. 2023

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“…PSM provides sufficient signal‐to‐noise ratio for analysis of the single exosome binding events, and can be easily constructed on a popular prism‐coupled SPR system with commercially available components. Therefore, PSM could become an economical and powerful tool for clinical exosome analysis as well as exosome biology research such as exosome biomarker binding properties [54–55] …”

Section: Exosome Analysismentioning

confidence: 99%

Plasmonic Scattering Microscopy for Label‐Free Imaging of Molecular Binding Kinetics: From Single Molecules to Single Cells

2023

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Measuring molecular binding kinetics represents one of the most important tasks in molecular interaction analysis. Surface plasmon resonance (SPR) is a popular tool for analyzing molecular binding. Plasmonic scattering microscopy (PSM) is a newly developed SPR imaging technology, which detects the out‐of‐plane scattering of surface plasmons by analytes and has pushed the detection limit of label‐free SPR imaging down to a single‐protein level. In addition, PSM also allows SPR imaging with high spatiotemporal resolution, making it possible to analyze cellular response to the molecular bindings. In this Mini Review, we present PSM as a method of choice for chemical and biological imaging, introduce its theoretical mechanism, present its experimental schemes, summarize its exciting applications, and discuss its challenges as well as the promising future.

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“…The size and concentration of EXO can be characterized by nanoparticle tracking analysis (NTA) and transmission electron microscopy (TEM) [32]. Recently, plasmonic scattering microscopy has been applied to image exosomes and analyze biomarkers [33].…”

Section: Characterization and Isolation Of Msc-exomentioning

confidence: 99%

Mesenchymal Stem Cell-Derived Exosomes for Myocardial Infarction Treatment

Zheng¹,

Hong²,

Hu³

et al. 2023

Exosomes - Recent Advances From Bench to Bedside

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Myocardial infarction (MI) is a major cause of morbidity and mortality in modern society. Over the past decades, mesenchymal stem cell (MSCs)-based therapy has shown promising results in the treatment of MI due to their unique properties of multi-differentiation ability, immune-privileged phenotype and paracrine activity. Recently, MSC-derived exosomes (MSC-EXO) have been proposed as a promising therapeutic strategy for MI with their ability to inhibit cardiomyocyte apoptosis and stimulate vascular angiogenesis. They also aid immunoregulation and rejuvenation of cardiomyocyte senescence by transporting their unique content such as proteins, lipids, and miRNAs. Compared with MSC transplantation, MSC-EXO administration has shown several advantages, including lower toxicity and immunogenicity and no risk of tumor formation. Nonetheless the potential mechanisms underlying MSC-EXO-based therapy for MI are not fully understood. In addition, lack of modification of MSC-EXOs can impact therapeutic efficacy. It is vital to optimize MSC-EXO and enhance their therapeutic efficacy for MI. We summarize the recent advances regarding biological characteristics, therapeutic potential and mechanisms, and optimal approaches to the use of MSC-EXOs in the treatment of MI.

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Label-free imaging and biomarker analysis of exosomes with plasmonic scattering microscopy

Abstract: Exosome analysis is a promising tool for clinical and biological research applications. However, detection and biomarker quantification of exosomes is technically challenging because they are small and highly heterogeneous. Here...

Cited by 30 publications

References 54 publications

Label-free analysis of membrane protein binding kinetics and cell adhesions using evanescent scattering microscopy

Label-free analysis of membrane protein binding kinetics and cell adhesions using evanescent scattering microscopy

Plasmonic Scattering Microscopy for Label‐Free Imaging of Molecular Binding Kinetics: From Single Molecules to Single Cells

Mesenchymal Stem Cell-Derived Exosomes for Myocardial Infarction Treatment

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