Label-free imaging of 3D pluripotent stem cell differentiation dynamics on chip

Optical redox imaging to screen synthetic hydrogels for stem cell-derived cardiomyocyte differentiation and maturation

“…47,48 Other label-free imaging modalities can monitor structural changes that occur during stem cell differentiation. [49][50][51] Importantly, these techniques are repeatable and live cell-compatible, allowing traditional endpoint analyses to be performed on the same cell batches. iPSCs primarily use glycolysis for adenosine triphosphate (ATP) production and shift to oxidative phosphorylation during differentiation to CMs, 52,53 following the metabolic trends of fetal and adult CMs, respectively.…”