Label‐free imaging of the regenerating human periodontal ligament and gingival tissue using second harmonic generation confocal microscopy (LB41)

Abstract

Surgical treatment interventions for treating periodontal disease all disrupt the collagenous structures (gingiva and periodontal ligament) that form the soft‐tissue supporting structures of teeth. We hypothesized that using the second harmonic generation (SHG) technique would enable detection of changes in the appearance, fibril density, and fibril organizational structure of the human periodontal ligament and gingiva during the regenerative process taking place following periodontal surgery. De‐identified archival unstained,de‐paraffinized histological sections (4μM) of teeth with surrounding hard and soft tissues (from normal or various stages of post‐surgical regenerating periodontal structures) were used. It was found that SHG clearly showed the normal organization and the subsequent changes in the collagen fibrils in normal vs.regenerating periodontal soft tissues. This multidisciplinary study shows that SHG can be effectively used to study the organization of collagen in the normal and regenerating periodontal ligament and surrounding gingival tissue. SHG could constitute a potentially useful technique for determining the efficacy of periodontal regenerative strategies, and for assessing / studying peri‐implantitis using punch biopsies. Grant Funding Source: INBRE/BRIN P20GM103427

Entering, Linked with the Sphinx: Lysophosphatidic Acids Everywhere, All at Once, in the Oral System and Cancer

“…GPCR intracellular signaling has also been more recently confirmed by studies of the thyroid-stimulating hormone receptor (TSHR) [125]. Our laboratory has found LPA1-3 on intact human periodontal ligament and human gingival tissue nuclear membranes by confocal microscopy [126,127]); we posit that the findings of Zhu et al (2006) [124] likely also apply to the PDD system.…”