Label-free immunosensor based on graphene/polyaniline nanocomposite for neutrophil gelatinase-associated lipocalin detection

Yukird, Jutiporn; Wongtangprasert, Tossapon; Rangkupan, Ratthapol; Chailapakul, Orawon; Pisitkun, Trairak; Rodthongkum, Nadnudda

doi:10.1016/j.bios.2016.08.062

Cited by 73 publications

(25 citation statements)

References 40 publications

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“…In recent years, many studies have been reported to develop several NGAL rapid diagnostic immunoassays, including the photoelectrochemical immunosensor and three electrochemical immunosensors, the solid-phase proximity ligation assay and lateral flow assay [29]. In one study, immunosensor has been made by NGAL capture antibodies immobilized to screen-printed-modified carbon electrode and labeled addition of secondary antibody against NGA to PB-NP-decorated g-C3N4 nanosheets forming a sandwich on the SPCE [10].…”

Section: Discussionmentioning

confidence: 99%

“…The low efficiency of PEG fusion in conventional methods to causes difficulties in obtaining functional antibodies, we optimized electric fusion parameters that enabled enhancement of fusion efficiency to prepare of viable hybridomas, and obtained two anti-NGAL mAbs, one conjugated with EU-NPS while the other used as capture antibodies [24]. In recent years, many studies have been reported to develop several NGAL rapid diagnostic immunoassays, including the photoelectrochemical immunosensor and three electrochemical immunosensors, the solid-phase proximity ligation assay and lateral flow assay [29]. In one study, immunosensor has been made by NGAL capture antibodies immobilized to screen-printed-modified carbon electrode and labeled addition of secondary antibody against NGA to PB-NP-decorated g-C3N4 nanosheets forming a sandwich on the SPCE [10].…”

Section: Discussionmentioning

confidence: 99%

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Development of a Europium Nanoparticles Lateral Flow Immunoassay for NGAL Detection in Urine and Diagnosis of Acute Kidney Injury

Yin

Nie

Líu

et al. 2021

Preprint

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Background:AKI is related to severe adverse outcomes and mortality with Coronavirus Infection Disease 2019 (COVID-19) patients, that early diagnosed and intervened is imperative. Neutrophil gelatinase-associated lipocalin (NGAL) is one of the most promising biomarkers for detection of acute kidney injury (AKI), but current detection methods are inadequacy, so more rapid, convenient and accuracy methods are needed to detect NGAL for early diagnosis of AKI. Herein, we established a rapid, reliable and accuracy lateral flow immunoassay based on europium nanoparticles (Eu-NPS-LFIA) for the detection of NGAL in human urine specimens. Methods:A double-antibody sandwich immunofluorescent assay using europium doped nanoparticles was employed and the NGAL monoclonal antibodies conjugate as labels were generated by optimizing electric fusion parameters. Eighty-three urine samples were used to evaluate the clinical application efficiency of this method. Results:The quantitative detection range of NGAL in AKI was 1-3000 ng/mL, and the detection sensitization was 0.36 ng/mL. The CV of intra-assay and inter-assay were 2.57%-4.98% and 4.11%-7.83%, respectively. Meanwhile, the correlation coefficient between Eu-NPS-LFIA and ARCHITECT analyzer was significant (R2=0.9829, n=83, p<0.01). Conclusions:Thus, a faster and easier operation quantitative assay of NGAL for AKI has been established, which is very important and meaningful to diagnose the early AKI, suggesting that the assay can provide an early warning of final outcome of disease.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Development of a Europium Nanoparticles Lateral Flow Immunoassay for NGAL Detection in Urine and Diagnosis of Acute Kidney Injury

Yin

Nie

Líu

et al. 2021

Preprint

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“…Several immobilization methods such as self-assembled monolayers (SAM) [19], electropolymerization [20], site-directed techniques [21], silanization [22] and direct covalent binding [23] have been utilized in the fabrication of immunosensors to handle these problems [24]. Immunosensors offer a number of advantages over traditional analytical techniques, including portability, specificity, cheapness and real-time monitoring as well as having good versatility, robustness, selectivity and sensitivity depending on the transducer [25].…”

Section: Introductionmentioning

confidence: 99%

Development of a functional impedimetric immunosensor for accurate detection of thy- roid-stimulating hormone

Asav¹

2021

Turk J Chem

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Thyroid-stimulating hormone (TSH) that regulates the synthesis of thyroid gland hormones controlling all metabolism is one of the pituitary hormones. Determination of TSH is crucial for monitoring thyroid gland-related disorders and some metabolic diseases.In this study, a non-labeled immunosensor based on covalent immobilization of anti-TSH antibody by using the formation of self-assembled monolayers (SAM) of 4mercaptophenylacetic acid (4-MPA) and functionalization of carboxyl ends with 1ethyl-3-(3-dimetilaminopropil) carbodiimide (EDC)/N-Hydroxysuccinimide (NHS) was fabricated for detection of TSH. Immobilization steps including the concentration of 4-2 MPA, the concentration of anti-TSH antibody and duration of anti-TSH antibody incubation were optimized by utilizing electrochemical impedance spectroscopy. Under optimal conditions, a sensitive, rapid and accurate determination of TSH at a concentration range between 0.7 -3.5 mIU/L was accomplished with a notable linearity and LOD value as 0.034 mIU/L as well as reproducibility and repeatability. Moreover, for comparison, linear range experiments were also carried out by using other electrochemical methods including Linear Sweep Voltammetry (LSV), Cyclic Voltammetry (CV) and capacitance spectroscopy (C). Finally, the constructed immunosensor was used for analyzing TSH levels spiked in the artificial serum samples.

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“…Such non-covalent functionalization requires that molecules have π-conjugated systems which overlap the π orbitals of the graphene, e.g., aromatic compounds [ 20 ]. One example of a common aromatic amine compound used to this end in biosensor development is aniline, which has been used in biosensing for such applications as the electrochemical detection of human chorionic gonadotropin using graphene screen printed electrodes [ 21 ], detection of neutrophil gelatinase-associated lipocalin using graphene/polymerized aniline nanocomposites [ 22 ] and real-time detection of ammonia using graphene/polymerized aniline nanocomposite films [ 23 ]. Another example of an aromatic amine compound used in biosensor development is 1,5-diaminonaphthalene (DAN) which has been previously used for the electrochemical detection of chloramphenicol using edge plane pyrolytic graphite sensors [ 24 ] and the detection of sulfamethoxazole using glassy carbon electrodes [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

A Facile Method for the Non-Covalent Amine Functionalization of Carbon-Based Surfaces for Use in Biosensor Development

et al. 2020

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Affinity biosensors based on graphene field-effect transistor (GFET) or resistor designs require the utilization of graphene’s exceptional electrical properties. Therefore, it is critical when designing these sensors, that the electrical properties of graphene are maintained throughout the functionalization process. To that end, non-covalent functionalization may be preferred over covalent modification. Drop-cast 1,5-diaminonaphthalene (DAN) was investigated as a quick and simple method for the non-covalent amine functionalization of carbon-based surfaces such as graphene, for use in biosensor development. In this work, multiple graphene surfaces were functionalized with DAN via a drop-cast method, leading to amine moieties, available for subsequent attachment to receptor molecules. Successful modification of graphene with DAN via a drop-cast method was confirmed using X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and real-time resistance measurements. Successful attachment of receptor molecules also confirmed using the aforementioned techniques. Furthermore, an investigation into the effect of sequential wash steps which are required in biosensor manufacture, on the presence of the DAN layer, confirmed that the functional layer was not removed, even after multiple solvent exposures. Drop-cast DAN is thus, a viable fast and robust method for the amine functionalization of graphene surfaces for use in biosensor development.

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Label-free immunosensor based on graphene/polyaniline nanocomposite for neutrophil gelatinase-associated lipocalin detection

Cited by 73 publications

References 40 publications

Development of a Europium Nanoparticles Lateral Flow Immunoassay for NGAL Detection in Urine and Diagnosis of Acute Kidney Injury

Development of a Europium Nanoparticles Lateral Flow Immunoassay for NGAL Detection in Urine and Diagnosis of Acute Kidney Injury

Development of a functional impedimetric immunosensor for accurate detection of thy- roid-stimulating hormone

A Facile Method for the Non-Covalent Amine Functionalization of Carbon-Based Surfaces for Use in Biosensor Development

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