Label-free impedance biosensors for Point-of-Care diagnostics

Chuang, Cheng-Hsin; Shaikh, Muhammad Omar

doi:10.5599/obp.11.6

Cited by 8 publications

(21 citation statements)

References 72 publications

(80 reference statements)

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“…Similar to other electrochemical techniques, EIS technique needs at least two electrodes to measure the impedance of the electrochemical cell, or more specifically the electrode‐solution interface. [ 91 ] The design of the electrodes and the electrode configurations play crucial roles in determining the performance of the electrochemical cells. In general, there are three different types of electrode configurations, which are the two‐electrode system, three‐electrode system, and four‐electrode system ( Figure ).…”

Section: Printed Electrodes For Eismentioning

confidence: 99%

Potential of Printed Electrodes for Electrochemical Impedance Spectroscopy (EIS): Toward Membrane Fouling Detection

Goh

Tay

Lee

et al. 2021

Adv Elect Materials

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Electrochemical impedance spectroscopy (EIS), one of the techniques for electrochemical analysis, has been used for a wide range of applications especially chemical‐ and bio‐sensing. Besides, EIS is one of the few techniques that has been proven useful for membrane fouling detection. Electrodes are some of the most essential components for analytes detection applications with EIS. With the advances in printing technology, the fabrication of advanced printed electrode systems with miniature designs and improved sensitivity for electrochemical sensing is made possible. This review addresses recent advances in printed electrodes for electrochemical impedance spectroscopy with the emphasis placed on discussing the use of printed electrodes for membrane fouling detection. Common electrode designs and materials for electrochemical impedance spectroscopy are discussed, along with practical examples of these printed electrodes. The commonly used printing techniques for the fabrication of printed electrodes are also deliberated. The successful realization of printed electrodes for EIS requires careful design of electrodes, proper selection of electrode materials, as well as optimization of the printing process. It is expected that printed electrodes will be widely accepted for EIS sensing applications and will facilitate the creation of low‐cost high‐performance sensing devices.

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Section: Printed Electrodes For Eismentioning

confidence: 99%

Potential of Printed Electrodes for Electrochemical Impedance Spectroscopy (EIS): Toward Membrane Fouling Detection

Goh

Tay

Lee

et al. 2021

Adv Elect Materials

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“…As previously discussed, specificity is a major problem that can be solved by antibodies or artificially synthesized aptamers, still in the process of development. Implementation of bioimpedance sensing in Lab-on-Chip based technology for point-of-care (PoC) and need-of-care (NoC) devices [364] opens new perspectives to prevent of epidemic spread of infectious diseases in developing countries, and environmental and social disasters, everywhere. New implantable sensors can be designed as well on the bases of bioimpedance sensing [365].…”

Section: Future Challenges Of Bioimpedancementioning

confidence: 99%

“…Excellent perspectives are opening in detection of pathogens for preventing epidemic spread of infectious diseases in developing countries, and environmental and social disasters, everywhere. Implementation of bioimpedance sensing in Lab-on-Chip based technology for point-of-care (PoC) and need-of-care (NoC) devices [364] opens new perspectives for prevention of the abovementioned threats.…”

Section: Future Challenges Of Bioimpedancementioning

confidence: 99%

Fundamentals, Recent Advances, and Future Challenges in Bioimpedance Devices for Healthcare Applications

2019

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This work develops a thorough review of bioimpedance systems for healthcare applications. The basis and fundamentals of bioimpedance measurements are described covering issues ranging from the hardware diagrams to the configurations and designs of the electrodes and from the mathematical models that describe the frequency behavior of the bioimpedance to the sources of noise and artifacts. Bioimpedance applications such as body composition assessment, impedance cardiography (ICG), transthoracic impedance pneumography, electrical impedance tomography (EIT), and skin conductance are described and analyzed. A breakdown of recent advances and future challenges of bioimpedance is also performed, addressing topics such as transducers for biosensors and Lab-on-Chip technology, measurements in implantable systems, characterization of new parameters and substances, and novel bioimpedance applications.

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“…By measuring the later with an AC signal, both resistive and capacitive properties are estimated at high frequencies, which enables the label-free and non-intrusive detection of bacteria immobilized by specific bioreceptors. Indeed, their presence in the membrane affects both the global conductivity and permittivity [ 25 , 30 ].…”

Section: Introductionmentioning

confidence: 99%

Electrical Characterization of Cellulose-Based Membranes towards Pathogen Detection in Water

Brun¹,

Hauwaert²,

Leprince

et al. 2021

Biosensors

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Paper substrates are promising for development of cost-effective and efficient point-of-care biosensors, essential for public healthcare and environmental diagnostics in emergency situations. Most paper-based biosensors rely on the natural capillarity of paper to perform qualitative or semi-quantitative colorimetric detections. To achieve quantification and better sensitivity, technologies combining paper-based substrates and electrical detection are being developed. In this work, we demonstrate the potential of electrical measurements by means of a simple, parallel-plate electrode setup towards the detection of whole-cell bacteria captured in nitrocellulose (NC) membranes. Unlike current electrical sensors, which are mostly integrated, this plug and play system has reusable electrodes and enables simple and fast bacterial detection through impedance measurements. The characterized NC membrane was subjected to (i) a biofunctionalization, (ii) different saline solutions modelling real water samples, and (iii) bacterial suspensions of different concentrations. Bacterial detection was achieved in low conductivity buffers through both resistive and capacitive changes in the sensed medium. To capture Bacillus thuringiensis, the model microorganism used in this work, the endolysin cell-wall binding domain (CBD) of Deep-Blue, a bacteriophage targeting this bacterium, was integrated into the membranes as a recognition bio-interface. This experimental proof-of-concept illustrates the electrical detection of 107 colony-forming units (CFU) mL−1 bacteria in low-salinity buffers within 5 min, using a very simple setup. This offers perspectives for affordable pathogen sensors that can easily be reconfigured for different bacteria. Water quality testing is a particularly interesting application since it requires frequent testing, especially in emergency situations.

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Label-free impedance biosensors for Point-of-Care diagnostics

Cited by 8 publications

References 72 publications

Potential of Printed Electrodes for Electrochemical Impedance Spectroscopy (EIS): Toward Membrane Fouling Detection

Potential of Printed Electrodes for Electrochemical Impedance Spectroscopy (EIS): Toward Membrane Fouling Detection

Fundamentals, Recent Advances, and Future Challenges in Bioimpedance Devices for Healthcare Applications

Electrical Characterization of Cellulose-Based Membranes towards Pathogen Detection in Water

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