Label-free impedance detection of cancer cells from whole blood on an integrated centrifugal microfluidic platform

Nwankire, Charles; Venkatanarayanan, Anita; Glennon, Thomas; Keyes, Tia E.; Forster, Robert J.; Ducrée, Jens

doi:10.1016/j.bios.2014.12.049

Cited by 98 publications

(76 citation statements)

References 45 publications

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“…[17][18][19][20][21] However, despite the previously mentioned advantages of having electric contact to a microfluidic system, only few examples of centrifugal microfluidic platforms interfaced with electrical connections have been reported in the literature. [22][23][24][25][26] These examples are based on one of two different options:…”

Section: Introductionmentioning

confidence: 99%

“…either they use the centrifugal microfluidic system solely for sample pre-treatment without connecting the electrodes to the rotating platform (then connecting a potentiostat afterwards), 25,26 or they use an electrical component designed for transferring the electrical signal to (and from) the stationary readout to the rotating parts. [22][23][24] The last approach typically involves the use of carbon brushes sliding across a metal ring (usually copper or silver).…”

Section: Introductionmentioning

confidence: 99%

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Integrating electrochemical detection with centrifugal microfluidics for real-time and fully automated sample testing

et al. 2015

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Abstract. Here we present a robust, stable and low-noise experimental set-up for performing electrochemical detection on a centrifugal microfluidic platform. By using a low-noise electronic component (electrical slipring) it is possible to achieve continuous, on-line monitoring of electrochemical experiments, even when the microfluidic disc is spinning at high velocities. Automated sample handling is achieved by designing a microfluidic system to release analyte sequentially, utilizing on-disc passive valving. In addition, the microfluidic system is designed to trap and keep the liquid sample stationary during analysis. In this way it is possible to perform cyclic voltammetry (CV) measurements at varying spin speeds, without altering the electrochemical response. This greatly simplifies the interpretation and quantification of data. Finally, real-time and continuous monitoring of an entire electrochemical experiment, including all intermediate sample handling steps, is demonstrated by amperometric detection of on-disc mixing of analytes (PBS and ferricyanide).

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Integrating electrochemical detection with centrifugal microfluidics for real-time and fully automated sample testing

et al. 2015

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“…A label-free cancer cells detection and characterization electrochemical centrifugal microfluidic platform are developed (Figure 5c). [50] Gold electrode labeled anti-EpCAM antibody can capture the SKOV3 cancer cell. And the adsorption of cells changes the voltammetric response of the gold electrode which represents the existing of cancer cells.…”

Section: Thermoplasticsmentioning

confidence: 99%

Materials for Microfluidic Immunoassays: A Review

Mou

Jiang

2017

Adv Healthcare Materials

120

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Conventional immunoassays suffer from at least one of these following limitations: long processing time, high costs, poor user‐friendliness, technical complexity, poor sensitivity and specificity. Microfluidics, a technology characterized by the engineered manipulation of fluids in channels with characteristic lengthscale of tens of micrometers, has shown considerable promise for improving immunoassays that could overcome these limitations in medical diagnostics and biology research. The combination of microfluidics and immunoassay can detect biomarkers with faster assay time, reduced volumes of reagents, lower power requirements, and higher levels of integration and automation compared to traditional approaches. This review focuses on the materials‐related aspects of the recent advances in microfluidics‐based immunoassays for point‐of‐care (POC) diagnostics of biomarkers. We compare the materials for microfluidic chips fabrication in five aspects: fabrication, integration, function, modification and cost, and describe their advantages and drawbacks. In addition, we review materials for modifying antibodies to improve the performance of the reaction of immunoassay. We also review the state of the art in microfluidic immunoassays POC platforms, from the laboratory to routine clinical practice, and also commercial products in the market. Finally, we discuss the current challenges and future developments in microfluidic immunoassays.

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“…[150][151][152] The most common type of electrochemical detection used in microfluidic systems is amperometric detection. 150,153,154 In amperometric detection, the current produced with either the reduction or oxidation of an electroactive species is monitored.…”

Section: Electrochemical Detectionmentioning

confidence: 99%

Lab-on-a-CD: A Fully Integrated Molecular Diagnostic System

et al. 2016

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The field of centrifugal microfluidics has experienced tremendous growth during the past 15 years, especially in applications such as lab-on-a-disc (LoD) diagnostics. The strength of LoD systems lies in its potential for development into fully integrated sample-to-answer analysis systems. This review highlights the technologies necessary to develop the next generation of these systems. In addition to outlining valving and other fluid-handling operations, we discuss the recent advances and future outlook in four categories of LoD processes: reagent storage, sample preparation, nucleic acid amplification, and analyte detection strategies.

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Label-free impedance detection of cancer cells from whole blood on an integrated centrifugal microfluidic platform

Cited by 98 publications

References 45 publications

Integrating electrochemical detection with centrifugal microfluidics for real-time and fully automated sample testing

Integrating electrochemical detection with centrifugal microfluidics for real-time and fully automated sample testing

Materials for Microfluidic Immunoassays: A Review

Lab-on-a-CD: A Fully Integrated Molecular Diagnostic System

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