Label-free impedimetric glycan biosensor for quantitative evaluation interactions between pathogenic bacteria and mannose

Cui, Feiyun; Xu, Yanhua; Wang, Renjie; Liu, Haitao; Chen, Li; Zhang, Qing; Mu, Xiaojing

doi:10.1016/j.bios.2017.11.068

Cited by 40 publications

(15 citation statements)

References 33 publications

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“…The need to develop electrochemical sensors has become especially urgent for biological agent indication systems in which early detection or monitoring (for example, environmental safety, biosafety, or monitoring molecular markers of the infection process) is of great importance [71,73,[96][97][98].…”

Section: Electrochemical Label-free Biosensorsmentioning

confidence: 99%

“…Among the most widely used materials are thin polymer films, nanostructured metal oxides, self-organizing monolayers of organic molecules (SAM), and carbon nanostructures (nanotubes, fullerenes, graphenes) [50]. For example, SAMs are considered ideal material for immobilizing nucleic acids when constructing biosensors for detecting bacteria [50,84,98,99].…”

Section: Electrochemical Label-free Biosensorsmentioning

confidence: 99%

“…Aptamer (rGO-TiO 2 ) Electrochemical S. enterica Typhimurium, 10 1 -10 8 ufc/mL [98] Nucleic acids (DNA) Piezoelectric…”

Section: Electrochemical Label-free Biosensorsmentioning

confidence: 99%

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Label-Free Biosensors for Laboratory-Based Diagnostics of Infections: Current Achievements and New Trends

et al. 2020

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Infections pose a serious global public health problem and are a major cause of premature mortality worldwide. One of the most challenging objectives faced by modern medicine is timely and accurate laboratory-based diagnostics of infectious diseases. Being a key factor of timely initiation and success of treatment, it may potentially provide reduction in incidence of a disease, as well as prevent outbreak and spread of dangerous epidemics. The traditional methods of laboratory-based diagnostics of infectious diseases are quite time- and labor-consuming, require expensive equipment and qualified personnel, which restricts their use in case of limited resources. Over the past six decades, diagnostic technologies based on lateral flow immunoassay (LFIA) have been and remain true alternatives to modern laboratory analyzers and have been successfully used to quickly detect molecular ligands in biosubstrates to diagnose many infectious diseases and septic conditions. These devices are considered as simplified formats of modern biosensors. Recent advances in the development of label-free biosensor technologies have made them promising diagnostic tools that combine rapid pathogen indication, simplicity, user-friendliness, operational efficiency, accuracy, and cost effectiveness, with a trend towards creation of portable platforms. These qualities exceed the generally accepted standards of microbiological and immunological diagnostics and open up a broad range of applications of these analytical systems in clinical practice immediately at the site of medical care (point-of-care concept, POC). A great variety of modern nanoarchitectonics of biosensors are based on the use of a broad range of analytical and constructive strategies and identification of various regulatory and functional molecular markers associated with infectious bacterial pathogens. Resolution of the existing biosensing issues will provide rapid development of diagnostic biotechnologies.

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Section: Electrochemical Label-free Biosensorsmentioning

confidence: 99%

Section: Electrochemical Label-free Biosensorsmentioning

confidence: 99%

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Label-Free Biosensors for Laboratory-Based Diagnostics of Infections: Current Achievements and New Trends

et al. 2020

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“…The recognition elements used in these types of analytical devices include DNA, RNA, PNA, and aptamers. For example, nowadays the sensor technology of DNA hybridization based on electrochemical (impedance spectroscopy) and optical methods which recognizes the complementary target DNA chain of a pathogenic microorganism is gaining popularity, [ 46 , 47 , 71 , 93 , 94 ]. Due to the ease of use and high sensitivity, electrochemical label-free biosensors have become the most widely used ones for detecting infectious pathogens in recent years ( Table 3 ).…”

Section: Label-free Biosensorsmentioning

confidence: 99%

Biosensor Technologies in Medicine: from Detection of Biochemical Markers to Research into Molecular Targets (Review)

Andryukov¹,

Lyapun²,

Матосова³

et al. 2020

Sovrem Tehnol Med

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Infections are a major cause of premature death. Fast and accurate laboratory diagnostics of infectious diseases is a key condition for the timely initiation and success of treatment. Potentially, it can reduce morbidity, as well as prevent the outbreak and spread of dangerous epidemics. The traditional methods of laboratory diagnostics of infectious diseases are quite time- and labour-consuming, require expensive equipment and trained personnel, which is crucial within limited resources. The fast biosensor-based methods that combine the diagnostic capabilities of biomedicine with modern technological advances in microelectronics, optoelectronics, and nanotechnology make an alternative. The modern achievements in the development of label-free biosensors make them promising diagnostic tools that combine rapid detection of specific molecular markers, simplicity, ease-of-use, efficiency, accuracy, and cost-effectiveness with the tendency to the development of portable platforms. These qualities exceed the generally accepted standards of microbiological and immunological diagnostics and open up broad prospects for using these analytical systems in clinical practice directly at the site of medical care provision (point-of-care, POC concept). A wide variety of modern biosensor designs are based on the use of diverse formats of analytical and technological strategies, identification of various regulatory and functional molecular markers associated with infectious pathogens. The solution to the existing problems in biosensing will open up great prospects for these rapidly developing diagnostic biotechnologies.

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“…Also, electrochemical impedance spectroscopy (EIS) is another powerful and useful tool to measure interfacial electrical properties which can be used to monitor bio-recognition events occurring at the electrode surface [ 32 , 33 , 34 ]. EIS is a non-destructive technique where a small amplitude perturbation allows direct detection of the target molecules from measured impedance changes of the sensing layer without any label [ 35 , 36 ].…”

Section: Introductionmentioning

confidence: 99%

Impedance Technique-Based Label-Free Electrochemical Aptasensor for Thrombin Using Single-Walled Carbon Nanotubes-Casted Screen-Printed Carbon Electrode

Park

2022

Sensors

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An impedance technique-based aptasensor for the detection of thrombin was developed using a single-walled carbon nanotube (SWCNT)-modified screen-printed carbon electrode (SPCE). In this work, a thrombin-binding aptamer (TBA) as probe was used for the determination of thrombin, and that was immobilized on SWCNT through π–π interaction. In the presence of thrombin, the TBA on SWCNT binds with target thrombin, and the amount of TBA on the SWCNT surface decreases. The detachment of TBA from SWCNT will be affected by the concentration of thrombin and the remaining TBA on the SWCNT surface can be monitored by electrochemical methods. The TBA-modified SWCNT/SPCE sensing layer was characterized by cyclic voltammetry (CV). For the measurement of thrombin, the change in charge-transfer resistance (Rct) of the sensing interface was investigated using electrochemical impedance spectroscopy (EIS) with a target thrombin and [Fe(CN)6]3− as redox maker. Upon incubation with thrombin, a decrease of Rct change was observed due to the decrease in the repulsive interaction between the redox marker and the electrode surface without any label. A plot of Rct changes vs. the logarithm of thrombin concentration provides the linear detection ranges from 0.1 nM to 1 µM, with a ~0.02 nM detection limit.

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Label-free impedimetric glycan biosensor for quantitative evaluation interactions between pathogenic bacteria and mannose

Cited by 40 publications

References 33 publications

Label-Free Biosensors for Laboratory-Based Diagnostics of Infections: Current Achievements and New Trends

Label-Free Biosensors for Laboratory-Based Diagnostics of Infections: Current Achievements and New Trends

Biosensor Technologies in Medicine: from Detection of Biochemical Markers to Research into Molecular Targets (Review)

Impedance Technique-Based Label-Free Electrochemical Aptasensor for Thrombin Using Single-Walled Carbon Nanotubes-Casted Screen-Printed Carbon Electrode

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