Label-free measurement of wall shear stress in the brain venule and arteriole using dual-wavelength third-harmonic-generation line-scanning imaging

Cheng, Hui; Chen, Xinlin; Zhong, Jincheng; Li, Jia; Qiu, Ping; Wang, Ke

doi:10.1364/ol.472136

Cited by 3 publications

(2 citation statements)

References 30 publications

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“…(2) THG peaks on the left side of the emission spectra are clearly visible, which is due to the flowing red blood vessels inside the vessel. 25 For a fair comparison, THG signals in vivo have to be removed, which was accomplished by the 633 nm long-pass filter, yielding pure 3PF signals for subsequent comparisons.…”

Section: Resultsmentioning

confidence: 99%

Comparison of the penetration depth in mouse brain in vivo through 3PF imaging using AIE nanoparticle labeling and THG imaging within the 1700 nm window

Zhang,

Zhong,

Cheng

et al. 2024

Nanoscale Adv.

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Section: Resultsmentioning

confidence: 99%

Comparison of the penetration depth in mouse brain in vivo through 3PF imaging using AIE nanoparticle labeling and THG imaging within the 1700 nm window

Zhang,

Zhong,

Cheng

et al. 2024

Nanoscale Adv.

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“…This is because THG is an intrinsic signal 34 that is generated from three-photon excitation and it provides label-free contrast at junctions of biological materials with dissimilar nonlinear indices of refraction, for example, at lipid boundaries 35 or elastic fibers 38 . More generally, THG signals have found a variety of applications from mapping the structure of white matter axonal tracts and neuronal cell membranes 31 , 32 , to tracking blood flow dynamics 36 , 37 .…”

Section: Methodsmentioning

confidence: 99%

The Cousa objective: a long-working distance air objective for multiphoton imaging in vivo

Yu,

Adsit

et al. 2023

Nat Methods

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Multiphoton microscopy can resolve fluorescent structures and dynamics deep in scattering tissue and has transformed neural imaging, but applying this technique in vivo can be limited by the mechanical and optical constraints of conventional objectives. Short working distance objectives can collide with compact surgical windows or other instrumentation and preclude imaging. Here we present an ultra-long working distance (20 mm) air objective called the Cousa objective. It is optimized for performance across multiphoton imaging wavelengths, offers a more than 4 mm2 field of view with submicrometer lateral resolution and is compatible with commonly used multiphoton imaging systems. A novel mechanical design, wider than typical microscope objectives, enabled this combination of specifications. We share the full optical prescription, and report performance including in vivo two-photon and three-photon imaging in an array of species and preparations, including nonhuman primates. The Cousa objective can enable a range of experiments in neuroscience and beyond.

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In vivo label-free measurement of blood flow velocity symmetry based on dual line scanning third-harmonic generation microscopy excited at the 1700 nm window

Cheng

Zhong

Qiu

et al. 2023

J. Innov. Opt. Health Sci.

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Measurement of blood flow velocity is key to understanding physiology and pathology in vivo. While most measurements are performed at the middle of the blood vessel, little research has been done on characterizing the instantaneous blood flow velocity distribution. This is mainly due to the lack of measurement technology with high spatial and temporal resolution. Here, we tackle this problem with our recently developed dual-wavelength line-scan third-harmonic generation (THG) imaging technology. Simultaneous acquisition of dual-wavelength THG line-scanning signals enables measurement of blood flow velocities at two radially symmetric positions in both venules and arterioles in mouse brain in vivo. Our results clearly show that the instantaneous blood flow velocity is not symmetric under general conditions.

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Label-free measurement of wall shear stress in the brain venule and arteriole using dual-wavelength third-harmonic-generation line-scanning imaging

Cited by 3 publications

References 30 publications

Comparison of the penetration depth in mouse brain in vivo through 3PF imaging using AIE nanoparticle labeling and THG imaging within the 1700 nm window

Comparison of the penetration depth in mouse brain in vivo through 3PF imaging using AIE nanoparticle labeling and THG imaging within the 1700 nm window

The Cousa objective: a long-working distance air objective for multiphoton imaging in vivo

In vivo label-free measurement of blood flow velocity symmetry based on dual line scanning third-harmonic generation microscopy excited at the 1700 nm window

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