Label-free measuring and mapping of binding kinetics of membrane proteins in single living cells

Wang, Wei; Yang, Yuanyuan; Wang, Shaopeng; Nagaraj, Vinay J.; Liu, Qiang; Wu, Jie; Tao, Nongjian

doi:10.1038/nchem.1434

Cited by 211 publications

(219 citation statements)

References 40 publications

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“…AFM can operate in aqueous solutions, but it is usually too slow to follow fast binding of ligands with bacteria, and the scanning AFM probe may perturb the binding process. In this study, we present a plasmonic imaging technique Wang et al, 2010Wang et al, , 2012 ( Fig. 1a) to study and quantify the interactions of a single E. Coli O157:H7 cell with an antibody, and perform statistical analysis of the bacterial heterogeneity.…”

Section: Introductionmentioning

confidence: 99%

Plasmonic imaging of protein interactions with single bacterial cells

Syal

Wang

Shan

et al. 2015

Biosensors and Bioelectronics

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Quantifying the interactions of bacteria with external ligands is fundamental to the understanding of pathogenesis, antibiotic resistance, immune evasion, and mechanism of antimicrobial action. Due to inherent cell--to--cell heterogeneity in a microbial population, each bacterium interacts differently with its environment. This large variability is washed out in bulk assays, and there is a need of techniques that can quantify interactions of bacteria with ligands at the single bacterium level.In this work, we present a label--free and real--time plasmonic imaging technique to

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Section: Introductionmentioning

confidence: 99%

Plasmonic imaging of protein interactions with single bacterial cells

Syal

Wang

Shan

et al. 2015

Biosensors and Bioelectronics

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“…SPRM is an optical microscopy that we recently developed to image the local refractive index (RI) distribution with a temporal resolution up to microseconds and a spatial resolution around the diffraction limit (19)(20)(21). SPRM is capable of monitoring local H 2 concentration due to the large difference in the RI between H 2 O and H 2 .…”

mentioning

confidence: 99%

“…) was used to excite planar surface plasmons on the gold film with an objective-based total internal reflection configuration (19)(20)(21). Light reflected from the gold film was collected via the same objective and directed into a camera to form an SPRM image.…”

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confidence: 99%

Intermittent photocatalytic activity of single CdS nanoparticles

Fang

Jiang

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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Semiconductor photocatalysis holds promising keys to address various energy and environmental challenges. Most studies to date are based on ensemble analysis, which may mask critical photocatalytic kinetics in single nanocatalysts. Here we report a study of imaging photocatalytic hydrogen production of single CdS nanoparticles with a plasmonic microscopy in an in operando manner. Surprisingly, we find that the photocatalytic reaction switches on and off stochastically despite the fact that the illumination is kept constant. The on and off states follow truncated and full-scale power-law distributions in broad time scales spanning 3–4 orders of magnitude, respectively, which can be described with a statistical model involving stochastic reactions rates at multiple active sites. This phenomenon is analogous to fluorescence photoblinking, but the underlying mechanism is different. As individual nanocatalyst represents the elementary photocatalytic platform, the discovery of the intermittent nature of the photocatalysis provides insights into the fundamental photochemistry and photophysics of semiconductor nanomaterials, which is anticipated to substantially benefit broad application fields such as clean energy, pollution treatment, and chemical synthesis.

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“…Recently, surface plasmon resonance imaging (SPRI), which is capable of spatially distinguishing refractive index (RI), was applied for real-time imaging of living cell activation [6][7][8] . Yanase et al [9] detected the RI changes in individual living cells by means of SPRI.…”

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confidence: 99%

A surface plasmon resonance imaging system for the stimulated living cell analysis

Zhang

Chen

et al. 2015

Optoelectron. Lett.

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In this paper, a surface plasmon resonance imaging (SPRI) system for cell analysis is developed for obtaining the surface plasmon resonance (SPR) signal from the interactions between cells and different stimuli. The system is constructed with a red laser light source, a P-polarizer, a glass prism, a 5× objective lens, a charge coupled device (CCD) camera, a gold sensor chip, a polydimethylsiloxane (PDMS) reaction well and a mechanical scanning device. The system is applied to mapping living cells in response to stimuli by characterization of the refractive index (RI) changes. Cell responses to K + in KCl solutions with concentrations of 5 mmol/L, 20 mmol/L, 50 mmol/L and 100 mmol/L are collected, which indicates that the SPRI method can distinguish the concentration of the stimuli. Furthermore, cell responses to epidermal growth factor (EGF) and vascular endothelial growth factor (VEGF) are studied independently. The binding of EGF receptor (EGFR) and EGF is collected as the first signal, and the internal change in cells is recorded as the second signal. The cell response to VEGF is different from that to EGF, which indicates that the SPRI as a label-free, real-time, fast and quantitative method has a potential to distinguish the cell responses to different stimuli.There are many conventional techniques for the evaluation of cell status, such as fluorescence microscopy, flow cytometry and electrical impedance. Most of the cell based assays require tedious labeling methods [1] which are time-consuming and may affect normal cell functions. The development of time-resolved label-free methodologies for the measurement of cellular properties is crucial for the analysis of living cells [2] .Surface plasmon resonance (SPR) is an extremely sensitive optical technique for the study of molecular interactions with advantages of real-time, label-free and kinetics detection at the sensor interface [3][4][5] . Recently, surface plasmon resonance imaging (SPRI), which is capable of spatially distinguishing refractive index (RI), was applied for real-time imaging of living cell activation [6][7][8] . Yanase et al [9] detected the RI changes in individual living cells by means of SPRI. Moreover, the SPR response could be used to describe cell growth [10] and evaluate the effectiveness of antitumor drug on tumor cells [11] . When cells were stimulated by extracellular stimuli via receptors, various intracellular events were initiated, which were then detected by SPR quantitatively [12] .In this paper, a homemade SPRI system is developed for cell analyses. SPR signals are obtained from the interactions between cells and different stimuli, such as K + , epidermal growth factor (EGF) and vascular endothelial growth factor (VEGF). This method provides a label-free, real-time, fast and quantitative way to detect RI changes in living cells induced by stimuli. The homemade SPRI system for living cell analyses is based on the prism coupling mode of the Kretschmann structure. As shown in Fig.1, the proposed SPRI system consists of a red la...

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Label-free measuring and mapping of binding kinetics of membrane proteins in single living cells

Cited by 211 publications

References 40 publications

Plasmonic imaging of protein interactions with single bacterial cells

Plasmonic imaging of protein interactions with single bacterial cells

Intermittent photocatalytic activity of single CdS nanoparticles

A surface plasmon resonance imaging system for the stimulated living cell analysis

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