Label‐Free Microfluidic Manipulation of Particles and Cells in Magnetic Liquids

Zhao, Wujun; Cheng, Rui; Miller, J.; Mao, Leidong

doi:10.1002/adfm.201504178

Cited by 133 publications

(129 citation statements)

References 128 publications

Supporting

Mentioning

128

Contrasting

Unclassified

Order By: Relevance

“…Fortunately, recent advances in microfluidic devices, especially on-a-chip EV isolation systems, have come a long way towards achieving these goals. Furthermore, there have been promising advances in technology used in the manipulation of cells and particles other than EVs, among which one of the most promising is label-free microfluidic manipulation technology or negative magnetophoresis-based technology [122]. These technologies also have the potential to advance the current state of liquid biopsies.…”

Section: Discussion and Outlookmentioning

confidence: 99%

Microfluidics‐based on‐a‐chip systems for isolating and analysing extracellular vesicles

Guo

Tao

Dawn

2018

J of Extracellular Vesicle

150

111

View full text Add to dashboard Cite

Extracellular vesicles (EVs), which can be found in almost all body fluids, consist of a lipid bilayer enclosing proteins and nucleic acids from their cells of origin. EVs can transport their cargo to target cells and have therefore emerged as key players in intercellular communication. Their potential as either diagnostic and prognostic biomarkers or therapeutic drug delivery systems (DDSs) has generated considerable interest in recent years. However, conventional methods used to study EVs still have significant limitations including the time-consuming and low throughput techniques required, while at the same time the demand for better research tools is getting stronger and stronger. In the past few years, microfluidics-based technologies have gradually emerged and have come to play an essential role in the isolation, detection and analysis of EVs. Such technologies have several advantages, including low cost, low sample volumes, high throughput and precision. This review summarizes recent advances in microfluidics-based technologies, compares conventional and microfluidics-based technologies, and includes a brief survey of recent progress towards integrated “on-a-chip” systems. In addition, this review also discusses the potential clinical applications of “on-a-chip” systems, including both “liquid biopsies” for personalized medicine and DDS devices for precision medicine, and then anticipates the possible future participation of cloud-based portable disease diagnosis and monitoring systems, possibly with the participation of artificial intelligence (AI).

show abstract

Section: Discussion and Outlookmentioning

confidence: 99%

Microfluidics‐based on‐a‐chip systems for isolating and analysing extracellular vesicles

Guo

Tao

Dawn

2018

J of Extracellular Vesicle

150

111

View full text Add to dashboard Cite

show abstract

“…[46,47] Cell characteristics such as differences in cell size can be used for microfluidic cancer cell sorting without other accommodations for biochemical characteristics. [47,48] Biophysical properties such as size work well when isolating cancer cells from blood due to the slightly larger size of CTCs when compared to other cells found in whole blood. Similar criteria can be incorporated in microfluidic platforms designed to screen out glioma cells to assess the invasive potential or to inform prognosis of the patient.…”

Section: Microfluidic Technologies For Gbmmentioning

confidence: 99%

“…[50] This technique was further adjusted for extracting CTCs from blood samples of patients, capable of accurately processing milliliters of whole blood in short periods of time using essential parameters such as flow velocity and shear force to influence efficiency of separation. [43,47] Cells thus sorted can subsequently be analyzed for mutations, secreted proteins, and drug resistance pathways to better inform treatment schemes or contribute to the design of novel therapeutic agents.…”

Section: Microfluidic Technologies For Gbmmentioning

confidence: 99%

Microfluidics in Malignant Glioma Research and Precision Medicine

et al. 2018

Self Cite

View full text Add to dashboard Cite

Glioblastoma multiforme (GBM) is an aggressive form of brain cancer that has no effective treatments and a prognosis of only 12–15 months. Microfluidic technologies deliver microscale control of fluids and cells, and have aided cancer therapy as point-of-care devices for the diagnosis of breast and prostate cancers. However, a few microfluidic devices are developed to study malignant glioma. The ability of these platforms to accurately replicate the complex microenvironmental and extracellular conditions prevailing in the brain and facilitate the measurement of biological phenomena with high resolution and in a high-throughput manner could prove useful for studying glioma progression. These attributes, coupled with their relatively simple fabrication process, make them attractive for use as point-of-care diagnostic devices for detection and treatment of GBM. Here, the current issues that plague GBM research and treatment, as well as the current state of the art in glioma detection and therapy, are reviewed. Finally, opportunities are identified for implementing microfluidic technologies into research and diagnostics to facilitate the rapid detection and better therapeutic targeting of GBM.

show abstract

“…Microfluidic manipulation of cells in magnetic liquids, 1 i.e., negative magnetophoresis, led to a number of recent applications in cell separation, 1–4 trapping and focusing, 5–8 and density measurements. 9–13 Its working principle is as follows: cells without any labels placed inside a uniformly magnetic media – magnetic liquids, act as “magnetic holes”.…”

Section: Introductionmentioning

confidence: 99%

Biocompatible and label-free separation of cancer cells from cell culture lines from white blood cells in ferrofluids

et al. 2017

Self Cite

View full text Add to dashboard Cite

This paper reports a biocompatible and label-free cell separation method using ferrofluids that can separate a variety of low-concentration cancer cells from cell culture lines (~100 cancer cells/mL) from undiluted white blood cells, with a throughput of 1.2 mL/h and an average separation efficiency of 82.2%. The separation is based on size difference from cancer cells and white blood cells, and is conducted in a custom-made biocompatible ferrofluid that retains not only excellent short-term viabilities, but also normal proliferations of 7 commonly used cancer cell lines. A microfluidic device is designed and optimized specifically to shorten the exposure time of live cells in ferrofluids from hours to seconds, by eliminating time-consuming off-chip sample preparation and extraction steps and integrating them on-chip to achieve one-step process. As a proof-of-concept demonstration, a ferrofluid with 0.26% volume fraction was used in this microfluidic device to separate spiked cancer cells from cell lines at a concentration of ~100 cells/mL from white blood cells with a 1.2 mL/h throughput. The separation efficiencies were 80±3%, 81±5%, 82±5%, 82±4%, and 86±6% for A549 lung cancer, H1299 lung cancer, MCF-7 breast cancer, MDA-MB-231 breast cancer, and PC-3 prostate cancer cell lines, respectively. Separated cancer cells purity was between 25.3% and 28.8%. In addition, separated cancer cells from this strategy showed an average short-term viability of 94.4±1.3% and separated cells were cultured and demonstrated normal proliferation to the confluence even after the separation process. Owning to its excellent biocompatibility and label-free operation, and its ability to recover low concentration of cancer cells from white blood cells, this method could lead to a promising tool for rare cell separation.

show abstract

Label‐Free Microfluidic Manipulation of Particles and Cells in Magnetic Liquids

Cited by 133 publications

References 128 publications

Microfluidics‐based on‐a‐chip systems for isolating and analysing extracellular vesicles

Microfluidics‐based on‐a‐chip systems for isolating and analysing extracellular vesicles

Microfluidics in Malignant Glioma Research and Precision Medicine

Biocompatible and label-free separation of cancer cells from cell culture lines from white blood cells in ferrofluids

Contact Info

Product

Resources

About