Label-free monitoring of cell death induced by oxidative stress in living human cells using terahertz ATR spectroscopy

Zou, Yi; Liu, Qiao; Xia, Yang; Huang, Huachuan; Li, Jiang; Du, Liang-Hui; Li, Zeren; Zhao, Jianheng; Zhu, Li-Guo

doi:10.1364/boe.9.000014

Cited by 50 publications

(14 citation statements)

References 27 publications

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“…However, there is no relevant experimental evidence to prove it. With the development of nanomaterials, nanotechnology and optics (especially nano-bionic materials and ultrafast optical [79][80][81][82][83][84][85][86][87]), it is possible to provide direct experimental evidence for the relevant hypotheses.…”

Section: Summary and Discussionmentioning

confidence: 99%

A new viewpoint and model of neural signal generation and transmission: Signal transmission on unmyelinated neurons

Xiang

Tang

Chang³

et al. 2020

Nano Res.

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We establish a preliminary model of neural signal generation and transmission based on our previous research, and use this model to study signal transmission on unmyelinated nerves. In our model, the characteristics of neural signals are studied both on a long-time and a short time scale. On the long-time scale, the model is consistent with the circuit model. On the short time scale, the neural system exhibits a THz and infrared electromagnetic oscillation but the energy envelope curve of the rapidly oscillating signal varies slowly. In addition, the numerical method is used to solve the equations of neural signal generation and transmission, and the effects of the temperature on signal transmission are studied. It is found that overly high and overly low temperatures are not conducive to the transmission of neural signals.

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Section: Summary and Discussionmentioning

confidence: 99%

A new viewpoint and model of neural signal generation and transmission: Signal transmission on unmyelinated neurons

Xiang

Tang

Chang³

et al. 2020

Nano Res.

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“…The cell layers displayed a 6% to 8% peak-amplitude relative contrast compared to the medium alone. In 2018, Zou et al monitored oxidative stress response of living human cells based on ATR THz time-domain spectroscopy [30]. As shown in Fig.…”

Section: Treatment Of Biomedical Samplementioning

confidence: 99%

Terahertz spectroscopy in biomedical field: a review on signal-to-noise ratio improvement

et al. 2020

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With the non-ionizing, non-invasive, high penetration, high resolution and spectral fingerprinting features of terahertz (THz) wave, THz spectroscopy has great potential for the qualitative and quantitative identification of key substances in biomedical field, such as the early diagnosis of cancer, the accurate boundary determination of pathological tissue and non-destructive detection of superficial tissue. However, biological samples usually contain various of substances (such as water, proteins, fat and fiber), resulting in the signal-to-noise ratio (SNR) for the absorption peaks of target substances are very small and then the target substances are hard to be identified. Here, we present recent works for the SNR improvement of THz signal. These works include the usage of attenuated total reflection (ATR) spectroscopy, the fabrication of sample-sensitive metamaterials, the utilization of different agents (including contrast agents, optical clearing agents and aptamers), the application of reconstruction algorithms and the optimization of THz spectroscopy system. These methods have been proven to be effective theoretically, but only few of them have been applied into actual usage. We also analyze the reasons and summarize the advantages and disadvantages of each method. At last, we present the prospective application of THz spectroscopy in biomedical field.

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“…Nevertheless, the detection of the trace amount of analyte in aqueous environment using THz spectroscopy has encountered strong constraints because of lacking specific fingerprint and strong absorption of water in the THz domain. Thus, methods such as THz attenuated total reflection spectroscopy(THz-ATR) 6,7 , microfluidic chips [8][9][10][11][12] and metamaterials [13][14][15][16] have been developed, which increases the interaction strength between the analyte and the incident THz waves significantly and hence allows for a much higher sensitivity and try to achieve sensing in a liquid environment. The THz-ATR is limited by its relatively poor accessibility, integration limitations and high cost.…”

Section: Introductionmentioning

confidence: 99%

Plasmonics-Nanofluidics Metamaterial: An Ultrasensitive Platform for Terahertz biosensing and Quantitative Measurement of Molecules

Xie¹,

He²,

Liu³

et al. 2020

Preprint

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Background: The terahertz spectroscopy has attracted enormous attention for label free chemical and biological sensing, due to the presence of molecular fingerprints and remote sensing capabilities. However, because of the strong absorption of THz waves in aqueous solutions, detecting liquid-phase biological samples by THz spectroscopy has encountered strong constraints. Results: Here, we demonstrate for the first time a rapid and label-free sensing for liquid-phase biological samples using THz spectroscopy based on microfluidic metasensors. The metasensors were integrated with microfluidic chip to precisely control the liquid sample thickness within 220 nanometer (MMS-N) and 50 micrometer (MMS-U). The metasensors include a periodic split ring resonator (SRR) with two resonant peaks in the THz range. The sensitivity(S) and corresponding figure of merit (FOM) of the two resonance peaks of MMS-N and MMS-U are analyzed with respect to different refractive index matching solution. Both the two resonance peaks of the metasensors are sensitive to the varies refractive index environment and high-frequency resonant of MMS-N shows the best sensitivity(~136GHz/RIU). In addition, the label-free biological sensing capability was conducted through qualitative and quantitative LDL and ox-LDL, where a shift of resonance frequency was observed as the LDL and ox-LDL concentration increased. Conclusions: The results indicated nanofluidic metasensor presented excellent sensing capability for liquid-phase molecular analysis by THz spectroscopy, and the proposed sensing system has the potentials to be developed as a rapid, label-free and highly sensitive detection tool for molecular analysis by THz sensing.

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Label-free monitoring of cell death induced by oxidative stress in living human cells using terahertz ATR spectroscopy

Cited by 50 publications

References 27 publications

A new viewpoint and model of neural signal generation and transmission: Signal transmission on unmyelinated neurons

A new viewpoint and model of neural signal generation and transmission: Signal transmission on unmyelinated neurons

Terahertz spectroscopy in biomedical field: a review on signal-to-noise ratio improvement

Plasmonics-Nanofluidics Metamaterial: An Ultrasensitive Platform for Terahertz biosensing and Quantitative Measurement of Molecules

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