Label-Free Nucleic Acid Biosensing Using Nanomaterial-Based Localized Surface Plasmon Resonance Imaging: A Review

Bonyár, Attila

doi:10.1021/acsanm.0c01457

Cited by 58 publications

(27 citation statements)

References 143 publications

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“…Changes either in the bulk refractive index of the surrounding liquid or gas or in molecular events such as bioreceptor–analyte binding on the surface of the nanoparticles cause a detectable redshift in their light extinction spectrum [ 3 ]. Several excellent review papers can be found that demonstrate how to utilize this effect in a large variety of sensing applications [ 4 ], from gas sensing [ 5 ] to chemical and biosensing [ 6 , 7 ], e.g., for biomarker [ 8 , 9 , 10 ] or nucleic acid detection [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Maximizing the Surface Sensitivity of LSPR Biosensors through Plasmon Coupling—Interparticle Gap Optimization for Dimers Using Computational Simulations

Bonyár

2021

Biosensors

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The bulk and surface refractive index sensitivities of LSPR biosensors, consisting of coupled plasmonic nanosphere and nano-ellipsoid dimers, were investigated by simulations using the boundary element method (BEM). The enhancement factor, defined as the ratio of plasmon extinction peak shift of multi-particle and single-particle arrangements caused by changes in the refractive index of the environment, was used to quantify the effect of coupling on the increased sensitivity of the dimers. The bulk refractive index sensitivity (RIS) was obtained by changing the dielectric medium surrounding the nanoparticles, while the surface sensitivity was modeled by depositing dielectric layers on the nanoparticle in an increasing thickness. The results show that by optimizing the interparticle gaps for a given layer thickness, up to ~80% of the optical response range of the nanoparticles can be utilized by confining the plasmon field between the particles, which translates into an enhancement of ~3–4 times compared to uncoupled, single particles with the same shape and size. The results also show that in these cases, the surface sensitivity enhancement is significantly higher than the bulk RI sensitivity enhancement (e.g., 3.2 times vs. 1.8 times for nanospheres with a 70 nm diameter), and thus the sensors’ response for molecular interactions is higher than their RIS would indicate. These results underline the importance of plasmonic coupling in the optimization of nanoparticle arrangements for biosensor applications. The interparticle gap should be tailored with respect to the size of the used receptor/target molecules to maximize the molecular sensitivity, and the presented methodology can effectively aid the optimization of fabrication technologies.

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Section: Introductionmentioning

confidence: 99%

Maximizing the Surface Sensitivity of LSPR Biosensors through Plasmon Coupling—Interparticle Gap Optimization for Dimers Using Computational Simulations

Bonyár

2021

Biosensors

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“…SPR, characterized by its label-free biomolecular quantification, is promising for POC nucleic acid testing through combination with microfluidic chip coated with plasmonic nanomaterials, exhibiting favorable features of excellent sensitivity and real-time information output ( Bonyár 2020 ). The detection principle is based on monitoring the refractive index change on the chip surface.…”

Section: Sample-in-quantitative-answer-outmentioning

confidence: 99%

Fully integrated microfluidic devices for qualitative, quantitative and digital nucleic acids testing at point of care

Bai

You

et al. 2021

Biosensors and Bioelectronics

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Benefiting from emerging miniaturized and equipment-free nucleic acid testing (NAT) technologies, fully integrated NAT devices at point of care (POC) with the capability of “sample-in-answer-out” are proceeding at a break-neck speed to eliminate complex operations and reduce the risk of contamination. Like the development of PCR technology (standard technique for NAT), the detection signal of fully integrated NAT devices has evolved from qualitative to quantitative and recently to digital readout, aiming at expanding their extensive applications through gradually improving detection sensitivity and accuracy. This review firstly introduces the existing commercial products, and then illustrates recent fully integrated microfluidic devices for NAT at POC from the aspect of detection signals ( i.e. , qualitative, quantitative and digital). Importantly, the key issues of existing commercial products and the main challenges between scientific research and product development are discussed. On this basis, we envision that the MARCHED ( m iniaturized, a utomatic, r eagent-preloaded, c ommercializable, h igh-throughput, e nvironment-independent and d isposable) NAT devices are expected to be realized in the near future.

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“…[38] When multiple nanoscale structures are combined together into an array, unique plasmonic capabilities can also be obtained as evidenced in nanoarray and nanolattice plasmonic biosensors, including nanohole and nanoslit arrays, nanodisks, and nanoparticle functionalized surfaces. [40][41][42] Other notable examples of nanoarray structures were outlined in a recent review by Jiang et al [40] These include nanocrescents, [43][44][45] nanocross and bar, [46,47] and nanobowls. [48][49][50] Another relatively common phenomena utilized in optical biosensing is SERS.…”

Section: Optical Biosensorsmentioning

confidence: 99%

Advances in Biosensors and Diagnostic Technologies Using Nanostructures and Nanomaterials

Welch

Powell

Clevinger

et al. 2021

Adv Funct Materials

114

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Nanoscale materials have unique properties that make them especially useful for biomedical diagnostic applications. Recent developments in nanoengineering have resulted in increasing use of nanostructures in biosensors. Various types of 0D, 1D, 2D, and 3D nanostructures have been used to improve biosensor sensitivity, selectivity, limit of detection, and time to result, among other metrics. These nanostructures have been integrated into electrochemical, optical, and other biosensors for this purpose. Here, the most recent advances in the use of nanostructured materials in biosensors are described. This includes a discussion of nanoparticles, nanorods, nanofibers, nanopillars, nanowires, nanosheets, indented nanopatterns (nanoholes and nanoslits), nanogaps, nanochannels, nanopores, nanofunctionalized surfaces, and complex hierarchical structures and their unique advantages and applications in biosensors. Clinical applications of these nanobiosensors are also highlighted along with a discussion of the future directions for these materials in diagnostics.

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Label-Free Nucleic Acid Biosensing Using Nanomaterial-Based Localized Surface Plasmon Resonance Imaging: A Review

Cited by 58 publications

References 143 publications

Maximizing the Surface Sensitivity of LSPR Biosensors through Plasmon Coupling—Interparticle Gap Optimization for Dimers Using Computational Simulations

Maximizing the Surface Sensitivity of LSPR Biosensors through Plasmon Coupling—Interparticle Gap Optimization for Dimers Using Computational Simulations

Fully integrated microfluidic devices for qualitative, quantitative and digital nucleic acids testing at point of care

Advances in Biosensors and Diagnostic Technologies Using Nanostructures and Nanomaterials

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