Label-free optical detection of single enzyme-reactant reactions and associated conformational changes

Kim, Eugene; Baaske, Martin D.; Schuldes, Isabel; Wilsch, Peter S.; Vollmer, Frank

doi:10.1126/sciadv.1603044

Cited by 90 publications

(89 citation statements)

References 28 publications

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“…This motion of the polymerase causes a change in the volume of the molecule overlapping with the near field of the WGM sensor over time, thus translating to a resonance shift Δλ Res . To reiterate, the data in [115] proves that the platform can investigate structural state transitions in single molecules without labels.…”

Section: Optoplasmonic Sensors: Single-molecule Detectionmentioning

confidence: 87%

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Advances in optoplasmonic sensors – combining optical nano/microcavities and photonic crystals with plasmonic nanostructures and nanoparticles

et al. 2017

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Nanophotonic device building blocks, such as optical nano/microcavities and plasmonic nanostructures, lie at the forefront of sensing and spectrometry of trace biological and chemical substances. A new class of nanophotonic architecture has emerged by combining optically resonant dielectric nano/microcavities with plasmonically resonant metal nanostructures to enable detection at the nanoscale with extraordinary sensitivity. Initial demonstrations include single-molecule detection and even single-ion sensing. The coupled photonic-plasmonic resonator system promises a leap forward in the nanoscale analysis of physical, chemical, and biological entities. These optoplasmonic sensor structures could be the centrepiece of miniaturised analytical laboratories, on a chip, with detection capabilities that are beyond the current state of the art. In this paper, we review this burgeoning field of optoplasmonic biosensors. We first focus on the state of the art in nanoplasmonic sensor structures, high quality factor optical microcavities, and photonic crystals separately before proceeding to an outline of the most recent advances in hybrid sensor systems. We discuss the physics of this modality in brief and each of its underlying parts, then the prospects as well as challenges when integrating dielectric nano/microcavities with metal nanostructures. In Section 5, we hint to possible future applications of optoplasmonic sensing platforms which offer many degrees of freedom towards biomedical diagnostics at the level of single molecules.

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Section: Optoplasmonic Sensors: Single-molecule Detectionmentioning

confidence: 87%

“…One of the first label-free optical methodologies for observing the conformational changes of an enzyme, e.g. single enzymereactant interactions, has appeared in the literature [115]. The motions/dynamics of the polymerase enzyme are thereby construed as shifts in the WGM wavelength Δλ Res .…”

Section: Optoplasmonic Sensors: Single-molecule Detectionmentioning

confidence: 99%

Advances in optoplasmonic sensors – combining optical nano/microcavities and photonic crystals with plasmonic nanostructures and nanoparticles

et al. 2017

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“…Single proteins of a few nanometres in size and below can now be routinely detected in solution [1][2][3][4]. Recently, these biosensors have been used to shed light on fundamental biophysics phenomena [1,5] and have been shown to have potential applications ranging from medical diagnostics [2] to high-resolution imaging [6] and environmental monitoring [1]. Among the currently developed sensors are whispering gallery mode (WGM) resonators [7,8] and plasmonic sensors [3,9].…”

Section: Introductionmentioning

confidence: 99%

Quantum noise limited nanoparticle detection with exposed-core fiber

Mauranyapin¹,

Madsen²,

Booth³

et al. 2019

Opt. Express

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Label-free biosensors are important tools for clinical diagnostics and for studying biology at the single molecule level. The development of optical label-free sensors has allowed extreme sensitivity, but can expose the biological sample to photodamage. Moreover, the fragility and complexity of these sensors can be prohibitive to applications. To overcome these problems, we develop a quantum noise limited exposed-core fiber sensor providing robust platform for label-free biosensing with a natural path toward microfluidic integration. We demonstrate the detection of single nanoparticles down to 25 nm in radius with optical intensities beneath known biophysical damage thresholds.

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“…Their structural dynamics are central to catalysis, molecular recognition and signaling. WGM sensors can observe and analyze the dynamics of such intricate systems [134]. Developing such capability is a breakthrough in optical detection technology.…”

Section: Whispering-gallery Mode Sensorsmentioning

confidence: 99%

Roadmap on optical sensors

Ferreira

Castro-Camus

Ottaway

et al. 2017

J. Opt.

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Sensors are devices or systems able to detect, measure and convert magnitudes from any domain to an electrical one. Using light as a probe for optical sensing is one of the most efficient approaches for this purpose. The history of optical sensing using some methods based on absorbance, emissive and florescence properties date back to the 16th century. The field of optical sensors evolved during the following centuries, but it did not achieve maturity until the demonstration of the first laser in 1960. The unique properties of laser light become particularly important in the case of laser-based sensors, whose operation is entirely based upon the direct detection of laser light itself, without relying on any additional mediating device. However, compared with freely propagating light beams, artificially engineered optical fields are in increasing demand for probing samples with very small sizes and/or weak light–matter interaction. Optical fiber sensors constitute a subarea of optical sensors in which fiber technologies are employed. Different types of specialty and photonic crystal fibers provide improved performance and novel sensing concepts. Actually, structurization with wavelength or subwavelength feature size appears as the most efficient way to enhance sensor sensitivity and its detection limit. This leads to the area of micro- and nano-engineered optical sensors. It is expected that the combination of better fabrication techniques and new physical effects may open new and fascinating opportunities in this area. This roadmap on optical sensors addresses different technologies and application areas of the field. Fourteen contributions authored by experts from both industry and academia provide insights into the current state-of-the-art and the challenges faced by researchers currently. Two sections of this paper provide an overview of laser-based and frequency comb-based sensors. Three sections address the area of optical fiber sensors, encompassing both conventional, specialty and photonic crystal fibers. Several other sections are dedicated to micro- and nano-engineered sensors, including whispering-gallery mode and plasmonic sensors. The uses of optical sensors in chemical, biological and biomedical areas are described in other sections. Different approaches required to satisfy applications at visible, infrared and THz spectral regions are also discussed. Advances in science and technology required to meet challenges faced in each of these areas are addressed, together with suggestions on how the field could evolve in the near future.

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Label-free optical detection of single enzyme-reactant reactions and associated conformational changes

Abstract: Single-molecule enzyme-reactant interactions and the linked conformational changes are monitored via an optical microcavity sensor.

Cited by 90 publications

References 28 publications

Advances in optoplasmonic sensors – combining optical nano/microcavities and photonic crystals with plasmonic nanostructures and nanoparticles

Advances in optoplasmonic sensors – combining optical nano/microcavities and photonic crystals with plasmonic nanostructures and nanoparticles

Quantum noise limited nanoparticle detection with exposed-core fiber

Roadmap on optical sensors

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