Label-free optical imaging of cell function and collagen structure for cell-based therapies

Scanning ion conductance microscopy revealed cisplatin-induced morphological changes related to apoptosis in single adenocarcinoma cells

“…[20][21][22][23] Therefore, there is a need for non-invasive and label-free methods for studying live cell morphology over time. 24,25 Also, the discovery of new methods for apoptosis studies will impact the understanding of apoptosis at cellular and tissue level. 26 Atomic force microscopy (AFM) is a label-free and wellestablished scanning probe microscopy (SPM) technique that offers high resolution imaging of the 3D topography of single cells, 27,28 but due to the likelihood of contact between the AFM tip and the cell membrane, imaging mammalian cell lines is challenging, as the cantilever tip can deform the cell membrane and only information about the cytoskeleton can be obtained.…”

“…20–23 Therefore, there is a need for non-invasive and label-free methods for studying live cell morphology over time. 24,25 Also, the discovery of new methods for apoptosis studies will impact the understanding of apoptosis at cellular and tissue level. 26…”