Label-Free Optical Imaging of Lymphatic Vessels Within Tissue Beds IN VIVO

Yousefi, Siavash; Zhi, Zhongwei; Wang, Ke

doi:10.1109/jstqe.2013.2278073

Cited by 16 publications

(20 citation statements)

References 77 publications

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“…As we know that the main role of vasculature system is to delivery oxygen, nutrition, fluid and signaling molecules required by living tissue, and the lymphatic system usually developed in parallel to the blood vessels in the skin [69]. Kalchenko et al showed that the combined application of LSCI and fluorescence intravital microscopy approaches provided synchronic in vivo images of blood vessels and lymph vessels [70].…”

Section: Discussionmentioning

confidence: 99%

Accessing to arteriovenous blood flow dynamics response using combined laser speckle contrast imaging and skin optical clearing

Shi¹,

Chen²,

Tuchin³

et al. 2015

Biomed. Opt. Express

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Laser speckle contrast imaging (LSCI) shows a great potential for monitoring blood flow, but the spatial resolution suffers from the scattering of tissue. Here, we demonstrate the capability of a combination method of LSCI and skin optical clearing to describe in detail the dynamic response of cutaneous vasculature to vasoactive noradrenaline injection. Moreover, the superior resolution, contrast and sensitivity make it possible to rebuild arteries-veins separation and quantitatively assess the blood flow dynamical changes in terms of flow velocity and vascular diameter at single artery or vein level.

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Section: Discussionmentioning

confidence: 99%

Accessing to arteriovenous blood flow dynamics response using combined laser speckle contrast imaging and skin optical clearing

Shi¹,

Chen²,

Tuchin³

et al. 2015

Biomed. Opt. Express

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“…The origin of these reduced-scattering connected tubular structures in the skin has been already confirmed by intradermal injection of Evan's blue dye and monitoring the uptake path by surrounding lymph vessels into the sentinel lymph node. 23 Therefore, these lymph vessels can be visualized by a proper segmentation algorithm on OCT structure images. The details of our segmentation algorithm were previously published 24 and here, we will briefly explain the process.…”

Section: Segmentation Of Lymphatic Vesselsmentioning

confidence: 99%

Assessment of microcirculation dynamics during cutaneous wound healing phasesin vivousing optical microangiography

et al. 2014

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Cutaneous wound healing consists of multiple overlapping phases starting with blood coagulation following incision of blood vessels. We utilized label-free optical coherence tomography and optical microangiography (OMAG) to noninvasively monitor healing process and dynamics of microcirculation system in a mouse ear pinna wound model. Mouse ear pinna is composed of two layers of skin separated by a layer of cartilage and because its total thickness is around 500 μm, it can be utilized as an ideal model for optical imaging techniques. These skin layers are identical to human skin structure except for sweat ducts and glands. Microcirculatory system responds to the wound injury by recruiting collateral vessels to supply blood flow to hypoxic region. During the inflammatory phase, lymphatic vessels play an important role in the immune response of the tissue and clearing waste from interstitial fluid. In the final phase of wound healing, tissue maturation, and remodeling, the wound area is fully closed while blood vessels mature to support the tissue cells. We show that using OMAG technology allows noninvasive and label-free monitoring and imaging each phase of wound healing that can be used to replace invasive tissue sample histology and immunochemistry technologies.

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“…These studies in swine displayed the capability of the method to measure average velocity of tagged packets of ICG, but no spatially resolved information can be obtained, rendering the method unsuitable for accurately resolving the flow field in lymphatic vessels. Other noninvasive in vivo lymphangiography methods have been developed using an optical coherence tomography (OCT) approach or optical microangiography, a variant of OCT. 22,23 OCT lymphangiography allows for labelfree demarcation of lymphatic vessels and flow visualization. No quantitative information on flow in lymphatics was reported in these studies.…”

Section: Introductionmentioning

confidence: 99%

Microparticle image velocimetry approach to flow measurements in isolated contracting lymphatic vessels

et al. 2016

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We describe the development of an optical flow visualization method for resolving the flow velocity vector field in lymphatic vessels in vitro. The aim is to develop an experimental protocol for accurately estimating flow parameters, such as flow rate and shear stresses, with high spatial and temporal resolution. Previous studies in situ have relied on lymphocytes as tracers, but their low density resulted in a reduced spatial resolution whereas the assumption that the flow was fully developed in order to determine the flow parameters of interest may not be valid, especially in the vicinity of the valves, where the flow is undoubtedly more complex. To overcome these issues, we have applied the time-resolved microparticle image velocimetry (μ -PIV) technique, a well-established method that can provide increased spatial and temporal resolution that this transient flow demands. To that end, we have developed a custom light source, utilizing high-power light-emitting diodes, and associated control and image processing software. This paper reports the performance of the system and the results of a series of preliminary experiments performed on vessels isolated from rat mesenteries, demonstrating, for the first time, the successful application of the μ -PIV technique in these vessels.

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Label-Free Optical Imaging of Lymphatic Vessels Within Tissue Beds IN VIVO

Cited by 16 publications

References 77 publications

Accessing to arteriovenous blood flow dynamics response using combined laser speckle contrast imaging and skin optical clearing

Accessing to arteriovenous blood flow dynamics response using combined laser speckle contrast imaging and skin optical clearing

Assessment of microcirculation dynamics during cutaneous wound healing phasesin vivousing optical microangiography

Microparticle image velocimetry approach to flow measurements in isolated contracting lymphatic vessels

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