Label-free photoacoustic imaging of the cardio-cerebrovascular development in the embryonic zebrafish

Chen, Qian; Jin, Tian; Qi, Weizhi; Mo, Xianming; Xi, Lei

doi:10.1364/boe.8.002359

Cited by 32 publications

(28 citation statements)

References 30 publications

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“…Owing to their low cost, ease of care, and large colony size, zebrafish are rapidly gaining popularity as biological models for drug discovery 59 and the study of disease modeling 60 . Furthermore, early in their developmental stages wild type zebrafish larvae have optical properties which are conducive to photoacoustic imaging 61,62 . In wild type zebrafish larvae, the dominant PA chromophores in the visible regime are melanin and hemoglobin.…”

Section: Description Of the F-mode Techniquementioning

confidence: 99%

Photoacoustic F-Mode imaging for scale specific contrast in biological systems

et al. 2019

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In photoacoustic (PA) imaging, time domain reconstruction techniques are the current gold standard for image formation. While these techniques provide high-resolution spatial maps of optical absorption, they neglect the structural information encoded in the frequency domain of the broadband PA signals. In this work, we introduce a frequency domain technique for PA image formation, termed F-Mode. By leveraging information contained in the frequency content of PA signals, F-Mode can be used to generate images with scale-specific contrast. To demonstrate the robustness of our technique, we apply F-Mode to datasets acquired using both PA tomography and PA microscopy systems, utilizing linear array and singleelement transducers with central frequencies ranging from 40-400 MHz. Here we show that the technique can be used to: differentiate between vessels and microspheres of different size in phantoms, enhance visualization of organelles in cultured cells, and selectively display single blood vessels in vivo in zebrafish larvae.

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Section: Description Of the F-mode Techniquementioning

confidence: 99%

Photoacoustic F-Mode imaging for scale specific contrast in biological systems

et al. 2019

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“…A spherically focused high-frequency transducer (V3332, Olympus) with an active area of 3 mm, a focal length of 12.7 mm, a center frequency of 50 MHz, and a bandwidth of 70% was used to collect optoacoustic signals. In our system, the dual foci of optical excitation and ultrasonic detection were configured confocally to maximize the systemic sensitivity [27]. The optoacoustic signals were amplified by a homemade pre-amplifier at ~64 dB, and digitized by a high-speed data acquisition card (ATS-9325, Alazar Inc.) at a sampling rate of 250 MS/s.…”

Section: System Configurationmentioning

confidence: 99%

An opto-acousto-fluidic microscopic system with a high spatiotemporal resolution for microfluidic applications

et al. 2019

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In this work, we develop a new opto-acouto-fludic microsopic system, which employs a high-speed one-dimensional galvanometer scanner and an ultrafast pulse laser (600 kHz). The new system has achieved a high two-dimensional frame rate of up to 2500 Hz with a lateral resolution of 1.7 μm and an axial resolution of 36 μm at the imaging plane. To demonstrate the improved performance of the new system compared to our previous one, we carried out experiments to image the flowing droplets generated with T-junction and flow focusing configurations. We also successfully imaged dynamic migration of magneto particles subjected to non-uniform magnetic field in the microchannel. The results suggest that our new system has sufficient spatiotemporal resolutions to carry out studies for high throughput microfluidic applications.

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“…melanin and hemoglobin) zebrafish larvae are also excellent candidates for imaging with photoacoustic microscopy (PAM). Conventional transmission-mode optical-resolution photoacoustic microscopy (OR-PAM) approaches have been used to image the eye, vascular system, and heart [ 12 ] as well as cardio-cerebrovascular development [ 13 ] in vivo in pigment-supressed zebrafish larvae. Other techniques, such as spatial resolution-invariant PAM (SIRPAM) have been used to acquire whole-body images of pigmentation in 3 day-post-fertilization (dpf) larvae over a lateral resolution-invariant axial range of 1.8 mm [ 14 ].…”

Section: Introductionmentioning

confidence: 99%