Label-free photoacoustic ophthalmic angiography

Hu, Song; Rao, Bin; Maslov, Konstantin; Wang, Lihong V.

doi:10.1364/ol.35.000001

Cited by 135 publications

(128 citation statements)

References 6 publications

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“…Third, the combination of the high nonradiative quantum yield of hemoglobin, the perfect 100% sensitivity of PAM to optical absorption, and the enhanced tissue transparency enables imaging sensitivity down to the single RBC level with a low laser exposure (contrast-to-noise ratio is ϳ 100:1 with 570-nm laser excitation). 53 Fourth, the combination of the contrast-free preparation and low-level laser exposure enables noninvasive repetitive imaging of chronic processes including, but not limited to, temporal angiogenesis. With the present L-PAM, we were able to dissect neovascular elaboration in TetON-HIF-1 mice at the capillary level in the same animal for prolonged observation intervals (60 days, or more if necessary).…”

Section: Discussionmentioning

confidence: 99%

Conditional HIF-1 induction produces multistage neovascularization with stage-specific sensitivity to VEGFR inhibitors and myeloid cell independence

Oladipupo¹,

Hu²,

Santeford³

et al. 2011

Blood

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Section: Discussionmentioning

confidence: 99%

Conditional HIF-1 induction produces multistage neovascularization with stage-specific sensitivity to VEGFR inhibitors and myeloid cell independence

Oladipupo¹,

Hu²,

Santeford³

et al. 2011

Blood

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“…These functional capabilities make OR-PAM a powerful research tool for basic preclinical investigations of oxygen supply and delivery at capillary level. With the exception of opthalmic applications [119,121] clinical applications of OR-PAM are inevitably limited owing to the short penetration depth it provides.…”

Section: 'Optical Resolution' Photoacoustic Microscopymentioning

confidence: 99%

“…A dualmode OR-PAM-OCT instrument has been developed [120] and used to obtain in vivo images of the retinal vasculature, optic disc and underlying RPE ( figure 15(a-c)). As well as imaging retinal blood vessels in there is further scope to visualize the structure and sO 2 of the iris microvasculature and related abnormalities as shown in figure 15(d) [121]. Silverman et al [176] describe an OR-PAM scheme combined with a high-frequency pulse-echo ultrasound imaging capability.…”

Section: Skinmentioning

confidence: 99%

Biomedical photoacoustic imaging

2011

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Photoacoustic (PA) imaging, also called optoacoustic imaging, is a new biomedical imaging modality based on the use of laser-generated ultrasound that has emerged over the last decade. It is a hybrid modality, combining the high-contrast and spectroscopic-based specificity of optical imaging with the high spatial resolution of ultrasound imaging. In essence, a PA image can be regarded as an ultrasound image in which the contrast depends not on the mechanical and elastic properties of the tissue, but its optical properties, specifically optical absorption. As a consequence, it offers greater specificity than conventional ultrasound imaging with the ability to detect haemoglobin, lipids, water and other light-absorbing chomophores, but with greater penetration depth than purely optical imaging modalities that rely on ballistic photons. As well as visualizing anatomical structures such as the microvasculature, it can also provide functional information in the form of blood oxygenation, blood flow and temperature. All of this can be achieved over a wide range of length scales from micrometres to centimetres with scalable spatial resolution. These attributes lend PA imaging to a wide variety of applications in clinical medicine, preclinical research and basic biology for studying cancer, cardiovascular disease, abnormalities of the microcirculation and other conditions. With the emergence of a variety of truly compelling in vivo images obtained by a number of groups around the world in the last 2 -3 years, the technique has come of age and the promise of PA imaging is now beginning to be realized. Recent highlights include the demonstration of whole-body small-animal imaging, the first demonstrations of molecular imaging, the introduction of new microscopy modes and the first steps towards clinical breast imaging being taken as well as a myriad of in vivo preclinical imaging studies. In this article, the underlying physical principles of the technique, its practical implementation, and a range of clinical and preclinical applications are reviewed.

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“…The laser pulse energy was 40 nJ, which was reported to be eye-safe by different groups. 4,17 Because reflection-SLO and PAOM shared the same optical illumination, pulse energy was the same as that for PAOM only.…”

Section: Experimental Systemmentioning

confidence: 99%

Integrating photoacoustic ophthalmoscopy with scanning laser ophthalmoscopy, optical coherence tomography, and fluorescein angiography for a multimodal retinal imaging platform

Song¹,

Wei²,

Liu³

et al. 2012

J. Biomed. Opt.

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Abstract. Photoacoustic ophthalmoscopy (PAOM) is a newly developed retinal imaging technology that holds promise for both fundamental investigation and clinical diagnosis of several blinding diseases. Hence, integrating PAOM with other existing ophthalmic imaging modalities is important to identify and verify the strengths of PAOM compared with the established technologies and to provide the foundation for more comprehensive multimodal imaging. To this end, we developed a retinal imaging platform integrating PAOM with scanning laser ophthalmoscopy (SLO), spectral-domain optical coherence tomography (SD-OCT), and fluorescein angiography (FA). In the system, all the imaging modalities shared the same optical scanning and delivery mechanisms, which enabled registered retinal imaging from all the modalities. High-resolution PAOM, SD-OCT, SLO, and FA images were acquired in both albino and pigmented rat eyes. The reported in vivo results demonstrate the capability of the integrated system to provide comprehensive anatomic imaging based on multiple optical contrasts.

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Label-free photoacoustic ophthalmic angiography

Cited by 135 publications

References 6 publications

Conditional HIF-1 induction produces multistage neovascularization with stage-specific sensitivity to VEGFR inhibitors and myeloid cell independence

Conditional HIF-1 induction produces multistage neovascularization with stage-specific sensitivity to VEGFR inhibitors and myeloid cell independence

Biomedical photoacoustic imaging

Integrating photoacoustic ophthalmoscopy with scanning laser ophthalmoscopy, optical coherence tomography, and fluorescein angiography for a multimodal retinal imaging platform

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