Label-free piezoelectric biosensor for prognosis and diagnosis of Systemic Lupus Erythematosus

Nascimento, Noelle M. do; Juste-Dolz, Augusto; Grau-García, Elena; Román-Ivorra, Jose Andrés; Puchades, Rosa; Maquieira, Ángel; Morais, Sergi; Giménez-Romero, David

doi:10.1016/j.bios.2016.11.004

Cited by 38 publications

(17 citation statements)

References 24 publications

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“…The paper-based discs are disposable while the PCB substrate can be reused. In comparison to other rapid, sensitive and portable label-free biosensors for protein quantification that have recently been reported in the literature [16][17][18][19][20][21][22][23][24][25][26][27][28], the presented method has improved cost-effectiveness, simplification and ease of use. Thus, although an increased upper limit of detection for our presented methodology is suspected, further research should be carried out to determine whether it may be applicable for the measurement of protein biomarkers of a broadened range of concentrations.…”

Section: Analysis and Comparison With Elisa And Recently Developed Mementioning

confidence: 97%

Carbon Nanotube-Based Electrochemical Biosensor for Label-Free Protein Detection

et al. 2019

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There is a growing need for biosensors that are capable of efficiently and rapidly quantifying protein biomarkers, both in the biological research and clinical setting. While accurate methods for protein quantification exist, the current assays involve sophisticated techniques, take long to administer and often require highly trained personnel for execution and analysis. Herein, we explore the development of a label-free biosensor for the detection and quantification of a standard protein. The developed biosensors comprise carbon nanotubes (CNTs), a specific antibody and cellulose filtration paper. The change in electrical resistance of the CNT-based biosensor system was used to sense a standard protein, bovine serum albumin (BSA) as a proof-of-concept. The developed biosensors were found to have a limit of detection of 2.89 ng/mL, which is comparable to the performance of the typical ELISA method for BSA quantification. Additionally, the newly developed method takes no longer than 10 min to perform, greatly reducing the time of analysis compared to the traditional ELISA technique. Overall, we present a versatile, affordable, simplified and rapid biosensor device capable of providing great benefit to both biological research and clinical diagnostics.

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Section: Analysis and Comparison With Elisa And Recently Developed Mementioning

confidence: 97%

Carbon Nanotube-Based Electrochemical Biosensor for Label-Free Protein Detection

et al. 2019

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“…They should fundamentally comprise a transducer (electrochemical [78], piezoelectric [79], or optical [80]) and a recognition molecule, which interacts with an analyte. A number of optical sensing studies have been performed on sensors involving chemiluminescence [81], fluorescence [82], light absorption [83], and reflectance [84] that can be classified as label-based and label-free.…”

Section: Macromolecular Imprinted Sensorsmentioning

confidence: 99%

Molecular Imprinting of Macromolecules for Sensor Applications

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et al. 2017

Sensors

148

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Molecular recognition has an important role in numerous living systems. One of the most important molecular recognition methods is molecular imprinting, which allows host compounds to recognize and detect several molecules rapidly, sensitively and selectively. Compared to natural systems, molecular imprinting methods have some important features such as low cost, robustness, high recognition ability and long term durability which allows molecularly imprinted polymers to be used in various biotechnological applications, such as chromatography, drug delivery, nanotechnology, and sensor technology. Sensors are important tools because of their ability to figure out a potentially large number of analytical difficulties in various areas with different macromolecular targets. Proteins, enzymes, nucleic acids, antibodies, viruses and cells are defined as macromolecules that have wide range of functions are very important. Thus, macromolecules detection has gained great attention in concerning the improvement in most of the studies. The applications of macromolecule imprinted sensors will have a spacious exploration according to the low cost, high specificity and stability. In this review, macromolecules for molecularly imprinted sensor applications are structured according to the definition of molecular imprinting methods, developments in macromolecular imprinting methods, macromolecular imprinted sensors, and conclusions and future perspectives. This chapter follows the latter strategies and focuses on the applications of macromolecular imprinted sensors. This allows discussion on how sensor strategy is brought to solve the macromolecules imprinting.

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“…Sensitive and accurate biosensing systems for these biomarkers can not only significantly aid in the early diagnosis and clinical management of AIDs but also help to establish therapeutic strategies [ 4 ]. Biosensors for the detection of autoantibodies specific to AIDs, including antiphospholipid syndrome (APS) [ 91 , 92 , 93 , 94 ], rheumatoid arthritis (RA) [ 95 , 96 , 97 , 98 , 99 ], systemic lupus erythematosus (SLE) [ 100 , 101 , 102 , 103 , 104 , 105 , 106 , 107 ], multiple sclerosis (MS) [ 108 , 109 , 110 , 111 ], and celiac disease (CD) [ 112 , 113 , 114 , 115 , 116 , 117 , 118 , 119 , 120 , 121 , 122 , 123 , 124 ], are now constructed based on diverse techniques with many advantages, including high sensitivity and easy operation.…”

Section: Potential Applications Of Antibody Biosensing For Clinicamentioning

confidence: 99%

Construction and Potential Applications of Biosensors for Proteins in Clinical Laboratory Diagnosis

Jiang

2017

Sensors

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Biosensors for proteins have shown attractive advantages compared to traditional techniques in clinical laboratory diagnosis. In virtue of modern fabrication modes and detection techniques, various immunosensing platforms have been reported on basis of the specific recognition between antigen-antibody pairs. In addition to profit from the development of nanotechnology and molecular biology, diverse fabrication and signal amplification strategies have been designed for detection of protein antigens, which has led to great achievements in fast quantitative and simultaneous testing with extremely high sensitivity and specificity. Besides antigens, determination of antibodies also possesses great significance for clinical laboratory diagnosis. In this review, we will categorize recent immunosensors for proteins by different detection techniques. The basic conception of detection techniques, sensing mechanisms, and the relevant signal amplification strategies are introduced. Since antibodies and antigens have an equal position to each other in immunosensing, all biosensing strategies for antigens can be extended to antibodies under appropriate optimizations. Biosensors for antibodies are summarized, focusing on potential applications in clinical laboratory diagnosis, such as a series of biomarkers for infectious diseases and autoimmune diseases, and an evaluation of vaccine immunity. The excellent performances of these biosensors provide a prospective space for future antibody-detection-based disease serodiagnosis.

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Label-free piezoelectric biosensor for prognosis and diagnosis of Systemic Lupus Erythematosus

Cited by 38 publications

References 24 publications

Carbon Nanotube-Based Electrochemical Biosensor for Label-Free Protein Detection

Carbon Nanotube-Based Electrochemical Biosensor for Label-Free Protein Detection

Molecular Imprinting of Macromolecules for Sensor Applications

Construction and Potential Applications of Biosensors for Proteins in Clinical Laboratory Diagnosis

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