Label-free plasmonic biosensors for point-of-care diagnostics: a review

Soler, María; Huertas, César S.; Lechuga, Laura M.

doi:10.1080/14737159.2019.1554435

Cited by 181 publications

(134 citation statements)

References 67 publications

Supporting

Mentioning

134

Contrasting

Order By: Relevance

“…Such multi-analyte detection methods are interesting for a wide range of on-line applications, where the quantitative and rapid evidence of several analytes in parallel is necessary [1][2][3][4]. Biosensors with immobilized enzymes represent one class of such inexpensive and small analytical devices; they are by far the most commonly used signaling method for the detection of different analytes in medicine, food quality control, environmental monitoring, and research [5][6][7][8][9][10][11]. The immobilization of costly biomolecules (e.g., enzymes, DNA, and antibodies) onto the sensor surface often demands their reuse for at least several times.…”

Section: Introductionmentioning

confidence: 99%

Towards a Multi-Enzyme Capacitive Field-Effect Biosensor by Comparative Study of Drop-Coating and Nano-Spotting Technique

Molinnus

Beging

Lowis

et al. 2020

Sensors

View full text Add to dashboard Cite

Multi-enzyme immobilization onto a capacitive field-effect biosensor by nano-spotting technique is presented. The nano-spotting technique allows to immobilize different enzymes simultaneously on the sensor surface with high spatial resolution without additional photolithographical patterning. The amount of applied enzymatic cocktail on the sensor surface can be tailored. Capacitive electrolyte-insulator-semiconductor (EIS) field-effect sensors with Ta2O5 as pH-sensitive transducer layer have been chosen to immobilize the three different (pL droplets) enzymes penicillinase, urease, and glucose oxidase. Nano-spotting immobilization is compared to conventional drop-coating method by defining different geometrical layouts on the sensor surface (fully, half-, and quarter-spotted). The drop diameter is varying between 84 µm and 102 µm, depending on the number of applied drops (1 to 4) per spot. For multi-analyte detection, penicillinase and urease are simultaneously nano-spotted on the EIS sensor. Sensor characterization was performed by C/V (capacitance/voltage) and ConCap (constant capacitance) measurements. Average penicillin, glucose, and urea sensitivities for the spotted enzymes were 81.7 mV/dec, 40.5 mV/dec, and 68.9 mV/dec, respectively.

show abstract

Section: Introductionmentioning

confidence: 99%

Towards a Multi-Enzyme Capacitive Field-Effect Biosensor by Comparative Study of Drop-Coating and Nano-Spotting Technique

Molinnus

Beging

Lowis

et al. 2020

Sensors

View full text Add to dashboard Cite

show abstract

“…These SPR-based techniques can be used for label-free real-time detection of analyte without external labeling (e.g., fluorescent dye, enzymes, etc.) and thus hold extreme potential in POC biosensing [ 80 ]. Another approach of utilizing SPR is by exploiting the surface-enhanced Raman scattering (SERS) signal from the target analytes.…”

Section: Surface-plasmon-resonance-based Platformsmentioning

confidence: 99%

Detection of Bacterial and Viral Pathogens Using Photonic Point-of-Care Devices

et al. 2020

View full text Add to dashboard Cite

Infectious diseases caused by bacteria and viruses are highly contagious and can easily be transmitted via air, water, body fluids, etc. Throughout human civilization, there have been several pandemic outbreaks, such as the Plague, Spanish Flu, Swine-Flu, and, recently, COVID-19, amongst many others. Early diagnosis not only increases the chance of quick recovery but also helps prevent the spread of infections. Conventional diagnostic techniques can provide reliable results but have several drawbacks, including costly devices, lengthy wait time, and requirement of trained professionals to operate the devices, making them inaccessible in low-resource settings. Thus, a significant effort has been directed towards point-of-care (POC) devices that enable rapid diagnosis of bacterial and viral infections. A majority of the POC devices are based on plasmonics and/or microfluidics-based platforms integrated with mobile readers and imaging systems. These techniques have been shown to provide rapid, sensitive detection of pathogens. The advantages of POC devices include low-cost, rapid results, and portability, which enables on-site testing anywhere across the globe. Here we aim to review the recent advances in novel POC technologies in detecting bacteria and viruses that led to a breakthrough in the modern healthcare industry.

show abstract

“…Current challenges for biosensors are to improve their detection sensitivity and reduce size and operation cost. Handheld biosensors with cost-effective sensing substrates are highly desired for medical and environmental tests [13]. At the beginning of market development, biosensing products typically require time-consuming and costly labelling processes, where a receptor is used with a fluorescent molecule bound with the target analytes.…”

Section: Introductionmentioning

confidence: 99%

Plasmonic Metasensors Based on 2D Hybrid Atomically Thin Perovskite Nanomaterials

et al. 2020

View full text Add to dashboard Cite

In this work, we have designed highly sensitive plasmonic metasensors based on atomically thin perovskite nanomaterials with a detection limit up to 10−10 refractive index units (RIU) for the target sample solutions. More importantly, we have improved phase singularity detection with the Goos–Hänchen (GH) effect. The GH shift is known to be closely related to optical phase signal changes; it is much more sensitive and sharp than the phase signal in the plasmonic condition, while the experimental measurement setup is much more compact than that of the commonly used interferometer scheme to exact the phase signals. Here, we have demonstrated that plasmonic sensitivity can reach a record-high value of 1.2862 × 109 µm/RIU with the optimum configurations for the plasmonic metasensors. The phase singularity-induced GH shift is more than three orders of magnitude larger than those achievable in other metamaterial schemes, including Ag/TiO2 hyperbolic multilayer metamaterials (HMMs), metal–insulator–metal (MIM) multilayer waveguides with plasmon-induced transparency (PIT), and metasurface devices with a large phase gradient. GH sensitivity has been improved by more than 106 times with the atomically thin perovskite metasurfaces (1.2862 × 109 µm/RIU) than those without (918.9167 µm/RIU). The atomically thin perovskite nanomaterials with high absorption rates enable precise tuning of the depth of the plasmonic resonance dip. As such, one can optimize the structure to reach near zero-reflection at the resonance angle and the associated sharp phase singularity, which leads to a strongly enhanced GH lateral shift at the sensor interface. By integrating the 2D perovskite nanolayer into a metasurface structure, a strong localized electric field enhancement can be realized and GH sensitivity was further improved to 1.5458 × 109 µm/RIU. We believe that this enhanced electric field together with the significantly improved GH shift would enable single molecular or even submolecular detection for hard-to-identify chemical and biological markers, including single nucleotide mismatch in the DNA sequence, toxic heavy metal ions, and tumor necrosis factor-α (TNFα).

show abstract

Label-free plasmonic biosensors for point-of-care diagnostics: a review

Cited by 181 publications

References 67 publications

Towards a Multi-Enzyme Capacitive Field-Effect Biosensor by Comparative Study of Drop-Coating and Nano-Spotting Technique

Towards a Multi-Enzyme Capacitive Field-Effect Biosensor by Comparative Study of Drop-Coating and Nano-Spotting Technique

Detection of Bacterial and Viral Pathogens Using Photonic Point-of-Care Devices

Plasmonic Metasensors Based on 2D Hybrid Atomically Thin Perovskite Nanomaterials

Contact Info

Product

Resources

About