Label free polyaniline based impedimetric biosensor for detection of E. coli O157:H7 Bacteria

Chowdhury, Ankan Dutta; De, Anil K.; Chaudhuri, Chirosree Roy; Bandyopadhyay, Krishnan; Sen, Pintu

doi:10.1016/j.snb.2012.06.004

Cited by 120 publications

(66 citation statements)

References 35 publications

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“…Table 1 establishes the fact that the sensitivity of the electrode applying Ag@BSA nanoflower as a matrix of immunosensor is comparable to the other recently reported label-free bacterial sensors (references [18–24]). The broad linear range might be attributed to the good conductivity of the inner Ag nanomaterials and the outer BSA molecules hindering nonspecific adsorption.…”

Section: Resultssupporting

confidence: 74%

Label-Free 3D Ag Nanoflower-Based Electrochemical Immunosensor for the Detection of Escherichia coli O157:H7 Pathogens

et al. 2016

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It is highly desirable to develop a rapid and simple method to detect pathogens. Combining nanomaterials with electrochemical techniques is an efficient way for pathogen detection. Herein, a novel 3D Ag nanoflower was prepared via a biomineralization method by using bovine serum albumin (BSA) as a template. It was adopted as a sensing interface to construct an electrochemical bacteria immunosensor for the rapid detection of foodborne pathogens Escherichia coli (E. coli) O157:H7. Bacterial antibody was immobilized onto the surface of Ag nanoflowers through covalent conjugation. Electrochemical impedance spectroscopy (EIS) was used to detect and validate the resistance changes, where [Fe(CN)6]3−/4− acted as the redox probe. A linear relation between R et and E. coli concentration was obtained in the E. coli concentration range of 3.0 × 102–3.0 × 108 cfu mL−1. The as-prepared biosensor gave rise to an obvious response to E. coli but had no distinct response to Cronobacter sakazakii, methicillin-resistant Staphylococcus aureus (MRSA), Staphylococcus albus, Lactobacillus easei, and Shigella flexneri, revealing a high selectivity for the detection of the pathogens down to 100 cfu mL−1 in a short time. We believe that this BSA-conjugated 3D Ag nanoflowers could be used as a powerful interface material with good conductivity and biocompatibility for improving pathogen detection and treatment in the field of medicine, environment, and food safety.Electronic supplementary materialThe online version of this article (doi:10.1186/s11671-016-1711-3) contains supplementary material, which is available to authorized users.

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Section: Resultssupporting

confidence: 74%

Label-Free 3D Ag Nanoflower-Based Electrochemical Immunosensor for the Detection of Escherichia coli O157:H7 Pathogens

et al. 2016

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“…Detection of positional mismatch at three different position of the ssDNA by differential pulse voltammetric process In Fig. 2B, it is observed that the proposed sensor gave a sharp highest peak intensity of 0.5184 A at −0.165 V in its single stranded form (1). Fully complementary ds-DNA uptakes minimum MB and shows lowest peak current value of 0.184 A in Fig.…”

Section: Complementary Target Dna Detection By Differential Pulse Volmentioning

confidence: 93%

“…Highly selective electrochemical detection of DNA and discrimination between base pairs mismatch in double-stranded DNA sequences holds a great importance in a wide range of areas including clinical diagnosis, biological and genomic study, forensic and medical applications [1][2][3][4][5][6]. Many types of DNA detection or mutation biosensor methods have been employed currently.…”

Section: Introductionmentioning

confidence: 99%

Detection of positional mismatch in oligonucleotide by electrochemical method

Chowdhury

Sarkar

2014

Sensors and Actuators B: Chemical

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“…Additionally, a new type of electrochemical DNA biosensor based on magnetic beads that detect the uidA gene, which encodes the enzyme β-D-glucuronidase produced by E. coli, has been developed (Geng et al, 2011). Impedimetric sensing based on covalently-linked antibodies on a conducting polyaniline film surface is also possible using the antibody-antigen binding method and this approach is label-free, rapid, and inexpensive (Setterington and Alocilja, 2011;Chowdhury et al, 2012).…”

Section: Escherichia Colimentioning

confidence: 99%

Biosensors and their Applications in Food Safety: A Review

Yasmin¹,

Ahmed²,

Cho³

2016

Journal of Biosystems Engineering

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Background: Foodborne pathogens are a growing concern with respect to human illnesses and death. There is an increasing demand for improvements in global food safety. However, it is a challenge to detect and identify these harmful organisms in a rapid, responsive, suitable, and effective way. Results: Rapid developments in biosensor designs have contributed to the detection of foodborne pathogens and other microorganisms. Biosensors can automate this process and have the potential to enable fast analyses that are cost and time-effective. Various biosensor techniques are available that can identify foodborne pathogens and other health hazards. Conclusions: In this review, biosensor technology is briefly discussed, followed by a summary of foodborne pathogen detection using various transduction systems that exhibit specificity for particular foodborne pathogens. In addition, the recent application of biosensor technology to detect pesticides and heavy metals is briefly addressed.

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Label free polyaniline based impedimetric biosensor for detection of E. coli O157:H7 Bacteria

Cited by 120 publications

References 35 publications

Label-Free 3D Ag Nanoflower-Based Electrochemical Immunosensor for the Detection of Escherichia coli O157:H7 Pathogens

Label-Free 3D Ag Nanoflower-Based Electrochemical Immunosensor for the Detection of Escherichia coli O157:H7 Pathogens

Detection of positional mismatch in oligonucleotide by electrochemical method

Biosensors and their Applications in Food Safety: A Review

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